Restriction map for
pFN21AE1776
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaI BsaWI BseAI BsgI
BsiWI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MfeI MflI
MluI MroI MseI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshBI
Psp1406I RcaI RsrII SacI SacII
SalI SapI ScaI SexAI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tru9I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 393 GTATAC GT^[AC][GT]AC
AflIII 287 ACATGT A^C[GA][CT]GT
AlwNI 398 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 846 GGACC G^G[AT]CC
BalI 448 TGGCCA TGG^CCA
BbeI 70 GGCGCC GGCGC^C
BbrPI 559 CACGTG CAC^GTG
BglI 110 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 195 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 781 GAGGAG GAGGAG(10/8)
BsiYI 688 CCCATCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 688 CCCATCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 44 GTCTC GTCTC(1/5)
BsmBI 43 CGTCTC CGTCTC(1/5)
BspLU11I 287 ACATGT A^CATGT
BsrFI 106 GCCGGC [GA]^CCGG[CT]
BssHII 102 GCGCGC G^CGCGC
Bst1107I 393 GTATAC GTA^TAC
BstEII 597 GGTGACC G^GT[ACGT]ACC
BstPI 597 GGTGACC G^GT[ACGT]ACC
BstZ17I 393 GTATAC GTA^TAC
Cfr10I 106 GCCGGC [GA]^CCGG[CT]
DpnII 736 GATC ^GATC
DsaI 812 CCACGG C^C[GA][CT]GG
EagI 178 CGGCCG C^GGCCG
EarI 801 GAAGAG CTCTTC(1/4)
EclXI 178 CGGCCG C^GGCCG
Eco52I 178 CGGCCG C^GGCCG
EcoO65I 597 GGTGACC G^GT[ACGT]ACC
EcoT14I 359 CCTTGG C^C[AT][AT]GG
KasI 70 GGCGCC G^GCGCC
Ksp632I 801 GAAGAG CTCTTC(1/4)
MamI 195 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 736 GATC ^GATC
MluNI 448 TGGCCA TGG^CCA
MscI 448 TGGCCA TGG^CCA
NaeI 106 GCCGGC GCC^GGC
NarI 70 GGCGCC GG^CGCC
NdeII 736 GATC ^GATC
NgoMIV 106 GCCGGC G^CCGGC
NspI 287 ACATGT [GA]CATG^[CT]
PleI 270 GAGTC GAGTC(4/5)
PmaCI 559 CACGTG CAC^GTG
PmlI 559 CACGTG CAC^GTG
PshAI 330 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 112 CTGCAG CTGCA^G
PvuI 735 CGATCG CGAT^CG
PvuII 539 CAGCTG CAG^CTG
Sau3AI 736 GATC ^GATC
SbfI 111 CCTGCAGG CCTGCA^GG
SfcI 112 CTGCAG C^T[GA][CT]AG
SfiI 109 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 70 GGCGCC GGC^GCC
Sse8387I 111 CCTGCAGG CCTGCA^GG
StyI 359 CCTTGG C^C[AT][AT]GG
TaqI 738 TCGA T^CGA
TthHB8I 738 TCGA T^CGA
XcmI 56 CCACCTTCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 70 GGCGCC G[GA]^CG[CT]C
697 GGCGTC
AspHI 79 GTGCTC G[AT]GC[AT]^C
291 GTGCTC
BanI 70 GGCGCC G^G[CT][GA]CC
807 GGTGCC
BbsI 261 GTCTTC GAAGAC(2/6)
502 GTCTTC
BpuAI 261 GTCTTC GAAGAC(2/6)
502 GTCTTC
BsaAI 139 TACGTG [CT]AC^GT[GA]
559 CACGTG
BsaHI 70 GGCGCC G[GA]^CG[CT]C
697 GGCGTC
BsiEI 178 CGGCCG CG[GA][CT]^CG
735 CGATCG
BsiHKAI 79 GTGCTC G[AT]GC[AT]^C
291 GTGCTC
BstF5I 201 CATCC GGATG(2/0)
628 CATCC
FauI 4 GCGGG CCCGC(4/6)
18 GCGGG
FokI 201 CATCC GGATG(9/13)
628 CATCC
HaeII 70 GGCGCC [GA]GCGC^[CT]
132 GGCGCT
HgaI 42 GCGTC GACGC(5/10)
698 GCGTC
Hin1I 70 GGCGCC G[GA]^CG[CT]C
697 GGCGTC
MspA1I 91 CCGCTG C[AC]G^C[GT]G
539 CAGCTG
NlaIII 288 CATG CATG^
531 CATG
SfaNI 675 GATGC GCATC(5/9)
627 GCATC
Tsp509I 550 AATT ^AATT
765 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AfaI AluI BanII
BbvI BcnI BmyI BpmI BsaJI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI EaeI
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboII MnlI MspI
MvaI MvnI MwoI NciI NlaIV
RsaI Sau96I ScrFI TfiI TseI
Tsp45I TspRI