Restriction map for
pFN21AE4173
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssSI Bst1107I
BstBI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MunI NarI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfoI SfuI SmaI
SmlI SnaBI SpeI SspBI SspI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 1396 GACGTC GACGT^C
AccI 1685 GTCTAC GT^[AC][GT]AC
AcsI 1033 GAATTC [GA]^AATT[CT]
AcyI 1396 GACGTC G[GA]^CG[CT]C
AflIII 350 ACACGT A^C[GA][CT]GT
AgeI 1208 ACCGGT A^CCGGT
Alw44I 465 GTGCAC G^TGCAC
AocI 987 CCTAAGG CC^T[ACGT]AGG
Aor51HI 774 AGCGCT AGC^GCT
ApaLI 465 GTGCAC G^TGCAC
ApoI 1033 GAATTC [GA]^AATT[CT]
BsaHI 1396 GACGTC G[GA]^CG[CT]C
BsaWI 1208 ACCGGT [AT]^CCGG[AT]
BsmI 1569 GAATGC GAATGC(1/-1)
BsrDI 1620 CATTGC GCAATG(2/0)
BstEII 1181 GGTCACC G^GT[ACGT]ACC
BstPI 1181 GGTCACC G^GT[ACGT]ACC
BstYI 1849 AGATCC [GA]^GATC[CT]
Bsu36I 987 CCTAAGG CC^T[ACGT]AGG
DraIII 565 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 774 AGCGCT AGC^GCT
Eco81I 987 CCTAAGG CC^T[ACGT]AGG
EcoNI 1724 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1181 GGTCACC G^GT[ACGT]ACC
EcoRI 1033 GAATTC G^AATTC
Hin1I 1396 GACGTC G[GA]^CG[CT]C
MflI 1849 AGATCC [GA]^GATC[CT]
MslI 1221 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 136 GCCGGC GCC^GGC
NcoI 680 CCATGG C^CATGG
NgoMIV 136 GCCGGC G^CCGGC
NotI 975 GCGGCCGC GC^GGCCGC
PflMI 60 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1208 ACCGGT A^CCGGT
PpuMI 210 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 36 GAGCTC GAGCT^C
SapI 1816 GAAGAGC GCTCTTC(1/4)
SfiI 627 GGCCGTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 227 CGCCGGTG C[GA]^CCGG[CT]G
SphI 746 GCATGC GCATG^C
TfiI 580 GAATC G^A[AT]TC
Tsp509I 1034 AATT ^AATT
Van91I 60 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1849 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 1850 GATCC GGATC(4/5)
1839 GGATC
AvaI 33 CCCGAG C^[CT]CG[GA]G
51 CCCGAG
BalI 848 TGGCCA TGG^CCA
908 TGGCCA
BanI 1295 GGCACC G^G[CT][GA]CC
1659 GGCACC
BbrPI 85 CACGTG CAC^GTG
351 CACGTG
BcgI 126 CGACTTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1460 GCATGAGGGTCG
BcnI 558 CCGGG CC^[GC]GG
1752 CCCGG
BsaI 331 GAGACC GGTCTC(1/5)
1349 GAGACC
BsmBI 388 GAGACG CGTCTC(1/5)
1394 GAGACG
BsoBI 33 CCCGAG C^[CT]CG[GA]G
51 CCCGAG
DsaI 680 CCATGG C^C[GA][CT]GG
1664 CCGTGG
EagI 165 CGGCCG C^GGCCG
976 CGGCCG
EclXI 165 CGGCCG C^GGCCG
976 CGGCCG
Eco52I 165 CGGCCG C^GGCCG
976 CGGCCG
HgaI 390 GACGC GACGC(5/10)
721 GACGC
MluNI 848 TGGCCA TGG^CCA
908 TGGCCA
MscI 848 TGGCCA TGG^CCA
908 TGGCCA
NciI 558 CCGGG CC^[GC]GG
1752 CCCGG
PleI 1285 GACTC GAGTC(4/5)
1683 GAGTC
PmaCI 85 CACGTG CAC^GTG
351 CACGTG
PmlI 85 CACGTG CAC^GTG
351 CACGTG
SbfI 867 CCTGCAGG CCTGCA^GG
1299 CCTGCAGG
SexAI 878 ACCTGGT A^CC[AT]GGT
1328 ACCAGGT
Sse8387I 867 CCTGCAGG CCTGCA^GG
1299 CCTGCAGG
StuI 821 AGGCCT AGG^CCT
991 AGGCCT
XcmI 296 CCAACTGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1223 CCAAGGTGCTGCTGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaII BanII BbsI
BbvCI BbvI BglI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI