Restriction map for
pFN21AE6692
##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AhdI AocI Aor51HI ApoI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbsI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MfeI
MflI MluI MroI MseI MslI
MunI NcoI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 757 GACGTC GACGT^C
AgeI 797 ACCGGT A^CCGGT
Alw44I 673 GTGCAC G^TGCAC
AlwI 225 GATCC GGATC(4/5)
ApaI 254 GGGCCC GGGCC^C
ApaLI 673 GTGCAC G^TGCAC
AscI 317 GGCGCGCC GG^CGCGCC
BbvCI 687 CCTCAGC CCTCAGC(-5/-2)
BfaI 40 CTAG C^TAG
BsaWI 797 ACCGGT [AT]^CCGG[AT]
BseRI 310 GAGGAG GAGGAG(10/8)
BsgI 910 GTGCAG GTGCAG(16/14)
BsmAI 729 GTCTC GTCTC(1/5)
BstAPI 675 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 981 CATCC GGATG(2/0)
BstXI 401 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 688 CTCAG C^T[ACGT]AG
DpnII 225 GATC ^GATC
EcoT14I 401 CCAAGG C^C[AT][AT]GG
FokI 981 CATCC GGATG(9/13)
FseI 294 GGCCGGCC GGCCGG^CC
HinfI 472 GACTC G^A[ACGT]TC
HphI 828 GGTGA GGTGA(8/7)
MaeI 40 CTAG C^TAG
MaeIII 829 GTGAC ^GT[ACGT]AC
MboI 225 GATC ^GATC
MboII 554 TCTTC GAAGA(8/7)
NaeI 295 GCCGGC GCC^GGC
NdeII 225 GATC ^GATC
NgoMIV 295 GCCGGC G^CCGGC
NotI 566 GCGGCCGC GC^GGCCGC
NspI 854 ACATGC [GA]CATG^[CT]
PflMI 819 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 797 ACCGGT A^CCGGT
PleI 472 GACTC GAGTC(4/5)
PstI 810 CTGCAG CTGCA^G
PvuII 913 CAGCTG CAG^CTG
Sau3AI 225 GATC ^GATC
SfaNI 980 GCATC GCATC(5/9)
SfcI 810 CTGCAG C^T[GA][CT]AG
SfiI 411 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 749 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 457 CTCAAG C^T[CT][GA]AG
StuI 42 AGGCCT AGG^CCT
StyI 401 CCAAGG C^C[AT][AT]GG
Tsp45I 829 GTGAC ^GT[GC]AC
Van91I 819 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 822 GTAC GT^AC
933 GTAC
AlwNI 820 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
841 CAGAGCCTG
BalI 188 TGGCCA TGG^CCA
410 TGGCCA
BbrPI 234 CACGTG CAC^GTG
436 CACGTG
BsrBI 313 GAGCGG CCGCTC(-3/-3)
328 GAGCGG
KspI 564 CCGCGG CCGC^GG
804 CCGCGG
MluNI 188 TGGCCA TGG^CCA
410 TGGCCA
MscI 188 TGGCCA TGG^CCA
410 TGGCCA
NlaIII 414 CATG CATG^
855 CATG
PmaCI 234 CACGTG CAC^GTG
436 CACGTG
PmlI 234 CACGTG CAC^GTG
436 CACGTG
PpuMI 110 GGGACCC [GA]G^G[AT]CC[CT]
350 GGGACCT
RsaI 822 GTAC GT^AC
933 GTAC
SacII 564 CCGCGG CCGC^GG
804 CCGCGG
SexAI 668 ACCTGGT A^CC[AT]GGT
824 ACCTGGT
SmaI 106 CCCGGG CCC^GGG
258 CCCGGG
TaqI 446 TCGA T^CGA
785 TCGA
TspRI 179 CAGTG CA[GC]TG[ACGT][ACGT]^
958 CAGTG
TthHB8I 446 TCGA T^CGA
785 TCGA
XmaCI 106 CCCGGG C^CCGGG
258 CCCGGG
XmaI 106 CCCGGG C^CCGGG
258 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BpmI BsaAI BsaHI BsaJI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI KasI MaeII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIV Sau96I ScrFI SfoI
TseI