Restriction map for
pFN21AE6792
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccIII AciI
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AviII BalI BbeI BbrPI
BbsI BbvCI BbvI BcgI BpuAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BspDI
BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraII DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I FauI Fnu4HI
FseI FspI HaeII Hin1I HindIII
ItaI KasI KpnI KspI MamI
MluNI MroI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SgrAI SnaBI SphI
Sse8387I SspI SwaI TseI Tth111I
Van91I XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AseI 2094 ATTAAT AT^TAAT
AsnI 2094 ATTAAT AT^TAAT
AvaI 1919 CCCGGG C^[CT]CG[GA]G
AvaII 1534 GGACC G^G[AT]CC
AvrII 1806 CCTAGG C^CTAGG
BamHI 589 GGATCC G^GATCC
BglI 1543 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 14 AGATCT A^GATCT
BlnI 1806 CCTAGG C^CTAGG
BlpI 144 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 144 GCTGAGC GC^T[ACGT]AGC
BsaAI 1363 TACGTG [CT]AC^GT[GA]
BsaI 394 GGTCTC GGTCTC(1/5)
BseRI 1255 GAGGAG GAGGAG(10/8)
BsoBI 1919 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2217 TGTACA T^GTACA
BsrGI 2217 TGTACA T^GTACA
BstBI 1622 TTCGAA TT^CGAA
CelII 144 GCTGAGC GC^T[ACGT]AGC
CfoI 264 GCGC GCG^C
DrdI 1325 GACTTTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1146 CTCTTC CTCTTC(1/4)
EcoNI 1801 CCTGACCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 794 GATATC GAT^ATC
EcoT22I 384 ATGCAT ATGCA^T
HhaI 264 GCGC GCG^C
HinP1I 264 GCGC G^CGC
HincII 1576 GTTAAC GT[CT]^[GA]AC
HindII 1576 GTTAAC GT[CT]^[GA]AC
HpaI 1576 GTTAAC GTT^AAC
Ksp632I 1146 CTCTTC CTCTTC(1/4)
MfeI 1113 CAATTG C^AATTG
MluI 786 ACGCGT A^CGCGT
MunI 1113 CAATTG C^AATTG
NsiI 384 ATGCAT ATGCA^T
NspV 1622 TTCGAA TT^CGAA
PshBI 2094 ATTAAT AT^TAAT
SfuI 1622 TTCGAA TT^CGAA
SmaI 1919 CCCGGG CCC^GGG
SpeI 48 ACTAGT A^CTAGT
SspBI 2217 TGTACA T^GTACA
StuI 449 AGGCCT AGG^CCT
XbaI 942 TCTAGA T^CTAGA
XmaCI 1919 CCCGGG C^CCGGG
XmaI 1919 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 265 CGCG CG^CG
787 CGCG
BciVI 740 GGATAC GTATCC(6/5)
1360 GGATAC
BclI 633 TGATCA T^GATCA
1590 TGATCA
BpmI 2021 CTCCAG CTGGAG(16/14)
298 CTGGAG
BsmFI 578 GGGAC GGGAC(10/14)
179 GTCCC
BspHI 1011 TCATGA T^CATGA
1335 TCATGA
BspLU11I 468 ACATGT A^CATGT
1159 ACATGT
BstUI 265 CGCG CG^CG
787 CGCG
Cac8I 856 GCTCGC GC[ACGT]^[ACGT]GC
1664 GCAGGC
DsaI 1544 CCATGG C^C[GA][CT]GG
1790 CCATGG
EcoRI 1416 GAATTC G^AATTC
1619 GAATTC
FbaI 633 TGATCA T^GATCA
1590 TGATCA
HgaI 1097 GACGC GACGC(5/10)
1868 GCGTC
MslI 381 CACATGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2070 CATCATAATG
MvnI 265 CGCG CG^CG
787 CGCG
NcoI 1544 CCATGG C^CATGG
1790 CCATGG
PstI 196 CTGCAG CTGCA^G
1885 CTGCAG
RcaI 1011 TCATGA T^CATGA
1335 TCATGA
SexAI 1000 ACCTGGT A^CC[AT]GGT
1302 ACCAGGT
TaqI 1623 TCGA T^CGA
1654 TCGA
TthHB8I 1623 TCGA T^CGA
1654 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AcsI AfaI AflII AflIII AluI
AlwI ApoI BanI BanII BcnI
BfaI BfrI BmyI BsaJI BsgI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DraI EcoRII EcoT14I
FokI HaeIII HapII HinfI HpaII
HphI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I Tsp45I
Tsp509I TspRI XcmI XhoII