Restriction map for
pFN21AE6696
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BbrPI BcgI
BciVI BclI BfrI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI Eam1105I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 844 GACGTC GACGT^C
AcsI 949 GAATTT [GA]^AATT[CT]
AfaI 879 GTAC GT^AC
Aor51HI 1142 AGCGCT AGC^GCT
ApaI 70 GGGCCC GGGCC^C
ApoI 949 GAATTT [GA]^AATT[CT]
AvrII 267 CCTAGG C^CTAGG
BbeI 791 GGCGCC GGCGC^C
BfaI 268 CTAG C^TAG
BglII 92 AGATCT A^GATCT
BlnI 267 CCTAGG C^CTAGG
BsmAI 773 GTCTC GTCTC(1/5)
BsrBI 141 GAGCGG CCGCTC(-3/-3)
BstXI 998 CCAAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 514 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1178 CGGCCG C^GGCCG
EarI 666 CTCTTC CTCTTC(1/4)
EclXI 1178 CGGCCG C^GGCCG
Eco47III 1142 AGCGCT AGC^GCT
Eco52I 1178 CGGCCG C^GGCCG
HincII 847 GTCAAC GT[CT]^[GA]AC
HindII 847 GTCAAC GT[CT]^[GA]AC
HphI 1013 TCACC GGTGA(8/7)
KasI 791 GGCGCC G^GCGCC
Ksp632I 666 CTCTTC CTCTTC(1/4)
MaeI 268 CTAG C^TAG
NarI 791 GGCGCC GG^CGCC
PaeR7I 637 CTCGAG C^TCGAG
PvuI 18 CGATCG CGAT^CG
RsaI 879 GTAC GT^AC
SacI 979 GAGCTC GAGCT^C
SfiI 71 GGCCCATCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 791 GGCGCC GGC^GCC
TaqI 638 TCGA T^CGA
TfiI 456 GAATC G^A[AT]TC
Tsp509I 950 AATT ^AATT
TthHB8I 638 TCGA T^CGA
XcmI 995 CCACCAAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 637 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 583 CGCG CG^CG
788 CGCG
AflIII 59 ACGTGT A^C[GA][CT]GT
919 ACGTGT
AlwNI 119 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
736 CAGGGGCTG
BalI 50 TGGCCA TGG^CCA
992 TGGCCA
BanI 791 GGCGCC G^G[CT][GA]CC
1078 GGTGCC
BbsI 435 GAAGAC GAAGAC(2/6)
933 GAAGAC
BbvCI 1193 CCTCAGC CCTCAGC(-5/-2)
684 GCTGAGG
BpuAI 435 GAAGAC GAAGAC(2/6)
933 GAAGAC
BseRI 748 GAGGAG GAGGAG(10/8)
958 GAGGAG
BsrI 1103 ACTGG ACTGG(1/-1)
1160 CCAGT
BstUI 583 CGCG CG^CG
788 CGCG
BstYI 92 AGATCT [GA]^GATC[CT]
578 AGATCC
DrdI 376 GACCCCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
862 GACCCCTACGTC
DsaI 353 CCATGG C^C[GA][CT]GG
839 CCATGG
HgaI 1129 GACGC GACGC(5/10)
658 GCGTC
MflI 92 AGATCT [GA]^GATC[CT]
578 AGATCC
MluNI 50 TGGCCA TGG^CCA
992 TGGCCA
MscI 50 TGGCCA TGG^CCA
992 TGGCCA
MseI 626 TTAA T^TAA
953 TTAA
MslI 54 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1102 CACTGGAGTG
MvnI 583 CGCG CG^CG
788 CGCG
NcoI 353 CCATGG C^CATGG
839 CCATGG
PpuMI 120 AGGACCT [GA]G^G[AT]CC[CT]
473 AGGACCT
PstI 191 CTGCAG CTGCA^G
1117 CTGCAG
SmaI 67 CCCGGG CCC^GGG
110 CCCGGG
Tru9I 626 TTAA T^TAA
953 TTAA
XhoII 92 AGATCT [GA]^GATC[CT]
578 AGATCC
XmaCI 67 CCCGGG C^CCGGG
110 CCCGGG
XmaI 67 CCCGGG C^CCGGG
110 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcyI AluI AlwI AocI
AspHI AvaI AvaII BanII BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsgI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BssKI
BstAPI BstF5I BstNI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII ItaI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TseI Tsp45I
TspRI