Restriction map for
pFN21AE6690
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvrII BalI
BanI BbeI BbrPI BcgI BciVI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FokI FseI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflII 390 CTTAAG C^TTAAG
AflIII 213 ACATGT A^C[GA][CT]GT
Asp700I 4 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 600 GGACC G^G[AT]CC
AviII 296 TGCGCA TGC^GCA
BamHI 42 GGATCC G^GATCC
BanII 531 GGGCTC G[GA]GC[CT]^C
BbsI 424 GAAGAC GAAGAC(2/6)
BbvCI 763 CCTCAGC CCTCAGC(-5/-2)
BclI 372 TGATCA T^GATCA
BfaI 793 CTAG C^TAG
BfrI 390 CTTAAG C^TTAAG
BmyI 531 GGGCTC G[AGT]GC[ACT]^C
BpmI 536 CTCCAG CTGGAG(16/14)
BpuAI 424 GAAGAC GAAGAC(2/6)
BsaBI 373 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 244 GTCTC GTCTC(1/5)
Bsp1286I 531 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 411 TGTACA T^GTACA
BspLU11I 213 ACATGT A^CATGT
BspMI 183 ACCTGC ACCTGC(4/8)
BsrGI 411 TGTACA T^GTACA
Cac8I 565 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 297 GCGC GCG^C
Cfr13I 600 GGACC G^G[ACGT]CC
EcoT14I 480 CCAAGG C^C[AT][AT]GG
FbaI 372 TGATCA T^GATCA
FspI 296 TGCGCA TGC^GCA
HaeIII 252 GGCC GG^CC
HhaI 297 GCGC GCG^C
HinP1I 297 GCGC G^CGC
MaeI 793 CTAG C^TAG
MaeII 678 ACGT A^CGT
MamI 373 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 65 CAGCTG C[AC]G^C[GT]G
NlaIV 42 GGATCC GG[ACGT]^[ACGT]CC
NspI 213 ACATGT [GA]CATG^[CT]
PshAI 572 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 65 CAGCTG CAG^CTG
SapI 187 GCTCTTC GCTCTTC(1/4)
Sau96I 600 GGACC G^G[ACGT]CC
SexAI 684 ACCTGGT A^CC[AT]GGT
SfaNI 732 GATGC GCATC(5/9)
SspBI 411 TGTACA T^GTACA
StuI 251 AGGCCT AGG^CCT
StyI 480 CCAAGG C^C[AT][AT]GG
Tsp45I 842 GTGAC ^GT[GC]AC
XmnI 4 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 457 AAATTT [GA]^AATT[CT]
485 GAATTT
AlwNI 242 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
353 CAGAATCTG
ApoI 457 AAATTT [GA]^AATT[CT]
485 GAATTT
BsgI 818 CTGCAC GTGCAG(16/14)
92 GTGCAG
BsiYI 591 CCCAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
612 CCGAAACCGGG
BslI 591 CCCAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
612 CCGAAACCGGG
BstYI 42 GGATCC [GA]^GATC[CT]
378 GGATCT
DdeI 248 CTGAG C^T[ACGT]AG
764 CTCAG
EarI 188 CTCTTC CTCTTC(1/4)
282 GAAGAG
HindIII 158 AAGCTT A^AGCTT
871 AAGCTT
HphI 103 GGTGA GGTGA(8/7)
688 GGTGA
Ksp632I 188 CTCTTC CTCTTC(1/4)
282 GAAGAG
MaeIII 325 GTAAC ^GT[ACGT]AC
842 GTGAC
MflI 42 GGATCC [GA]^GATC[CT]
378 GGATCT
MslI 85 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
769 CATATATATG
NlaIII 214 CATG CATG^
472 CATG
SmlI 390 CTTAAG C^T[CT][GA]AG
491 CTCAAG
TaqI 337 TCGA T^CGA
741 TCGA
TspRI 275 CAGTG CA[GC]TG[ACGT][ACGT]^
345 CAGTG
TthHB8I 337 TCGA T^CGA
741 TCGA
XhoII 42 GGATCC [GA]^GATC[CT]
378 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI AlwI BbvI BcnI
BsaJI BsmFI BsrI BssKI BstNI
DpnII EcoRII Fnu4HI HapII HinfI
HpaII ItaI MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII PleI RsaI Sau3AI ScrFI
SfcI TfiI Tru9I TseI Tsp509I