##################################
# NO CUT ENZYMES (  226 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MfeI        MluI        MluNI       
MnlI        MroI        MscI        MseI        MslI        
MspA1I      MspI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AcsI           88  GAATTC               [GA]^AATT[CT]
AluI           31  AGCT                 AG^CT
ApoI           88  GAATTC               [GA]^AATT[CT]
BbvI          119  GCAGC                GCAGC(8/12)
BglII          50  AGATCT               A^GATCT
BpmI           12  CTGGAG               CTGGAG(16/14)
BsmAI          15  GAGAC                GTCTC(1/5)
BsrI           11  ACTGG                ACTGG(1/-1)
BstF5I         36  CATCC                GGATG(2/0)
BstYI          50  AGATCT               [GA]^GATC[CT]
DpnII          51  GATC                 ^GATC
EcoRI          88  GAATTC               G^AATTC
Fnu4HI        119  GCAGC                GC^[ACGT]GC
FokI           36  CATCC                GGATG(9/13)
HaeIII          6  GGCC                 GG^CC
HinfI          26  GACTC                G^A[ACGT]TC
ItaI          119  GCAGC                GC^[ACGT]GC
MaeII         125  ACGT                 A^CGT
MboI           51  GATC                 ^GATC
MflI           50  AGATCT               [GA]^GATC[CT]
NdeII          51  GATC                 ^GATC
PleI           26  GACTC                GAGTC(4/5)
Sau3AI         51  GATC                 ^GATC
SfaNI          73  GATGC                GCATC(5/9)
TseI          119  GCAGC                G^C[AT]GC
XhoII          50  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

BbsI           81  GAAGAC               GAAGAC(2/6)
              108  GAAGAC              
BpuAI          81  GAAGAC               GAAGAC(2/6)
              108  GAAGAC              
DdeI           28  CTCAG                C^T[ACGT]AG
              104  CTGAG               
MboII          81  GAAGA                GAAGA(8/7)
              108  GAAGA               
Tsp509I        89  AATT                 ^AATT
              132  AATT                
TspRI          65  CAGTG                CA[GC]TG[ACGT][ACGT]^
               77  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################