Restriction map for
pFN21AE6332
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI AhdI AocI ApoI AscI
Asp700I AspEI AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BsaBI BsaI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI Bst1107I BstEII BstPI BstZ17I
Bsu36I Cfr10I DraI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KspI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI SacI
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SwaI Tsp45I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 954 GGTACC G^GTACC
AccI 241 GTCTAC GT^[AC][GT]AC
AccIII 100 TCCGGA T^CCGGA
AcyI 211 GGCGTC G[GA]^CG[CT]C
Alw44I 873 GTGCAC G^TGCAC
ApaI 351 GGGCCC GGGCC^C
ApaLI 873 GTGCAC G^TGCAC
AseI 589 ATTAAT AT^TAAT
AsnI 589 ATTAAT AT^TAAT
Asp718I 954 GGTACC G^GTACC
AspHI 873 GTGCAC G[AT]GC[AT]^C
AspI 169 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 258 GAAGAC GAAGAC(2/6)
BbvCI 633 GCTGAGG CCTCAGC(-5/-2)
BglI 568 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 423 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 423 GCTGAGC GC^T[ACGT]AGC
BpuAI 258 GAAGAC GAAGAC(2/6)
BsaHI 211 GGCGTC G[GA]^CG[CT]C
BseAI 100 TCCGGA T^CCGGA
BsiHKAI 873 GTGCAC G[AT]GC[AT]^C
BsmAI 613 GTCTC GTCTC(1/5)
BsmFI 153 GTCCC GGGAC(10/14)
BspDI 676 ATCGAT AT^CGAT
BspEI 100 TCCGGA T^CCGGA
BssHII 447 GCGCGC G^CGCGC
BstBI 778 TTCGAA TT^CGAA
BstXI 404 CCACGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1181 AGATCC [GA]^GATC[CT]
CelII 423 GCTGAGC GC^T[ACGT]AGC
ClaI 676 ATCGAT AT^CGAT
CpoI 160 CGGTCCG CG^G[AT]CCG
DraIII 466 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Hin1I 211 GGCGTC G[GA]^CG[CT]C
HincII 913 GTCAAC GT[CT]^[GA]AC
HindII 913 GTCAAC GT[CT]^[GA]AC
HphI 672 GGTGA GGTGA(8/7)
KpnI 954 GGTACC GGTAC^C
MaeIII 485 GTTAC ^GT[ACGT]AC
MflI 1181 AGATCC [GA]^GATC[CT]
MroI 100 TCCGGA T^CCGGA
MseI 590 TTAA T^TAA
NcoI 266 CCATGG C^CATGG
NspV 778 TTCGAA TT^CGAA
PflFI 169 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 589 ATTAAT AT^TAAT
PstI 73 CTGCAG CTGCA^G
RsrII 160 CGGTCCG CG^G[AT]CCG
SapI 788 GAAGAGC GCTCTTC(1/4)
SfaNI 69 GATGC GCATC(5/9)
SfuI 778 TTCGAA TT^CGAA
SphI 800 GCATGC GCATG^C
SspI 131 AATATT AAT^ATT
Tru9I 590 TTAA T^TAA
Tsp509I 592 AATT ^AATT
Tth111I 169 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 659 CCAACTATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1181 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AlwNI 37 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
429 CAGCCACTG
Aor51HI 341 AGCGCT AGC^GCT
1019 AGCGCT
AvaI 509 CCCGGG C^[CT]CG[GA]G
583 CCCGGG
BalI 290 TGGCCA TGG^CCA
401 TGGCCA
BanII 351 GGGCCC G[GA]GC[CT]^C
791 GAGCCC
BfaI 224 CTAG C^TAG
565 CTAG
BpmI 206 CTGGAG CTGGAG(16/14)
337 CTGGAG
BsaAI 949 TACGTG [CT]AC^GT[GA]
985 TACGTG
BsaWI 20 TCCGGT [AT]^CCGG[AT]
100 TCCGGA
BseRI 118 GAGGAG GAGGAG(10/8)
388 GAGGAG
BsoBI 509 CCCGGG C^[CT]CG[GA]G
583 CCCGGG
BsrI 744 CCAGT ACTGG(1/-1)
1194 CCAGT
BssSI 469 CTCGTG CACGAG(-5/-1)
647 CTCGTG
BstAPI 428 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
875 GCACCCCCTGC
BstF5I 18 CATCC GGATG(2/0)
1147 CATCC
DdeI 424 CTGAG C^T[ACGT]AG
634 CTGAG
EarI 788 GAAGAG CTCTTC(1/4)
925 GAAGAG
Eco47III 341 AGCGCT AGC^GCT
1019 AGCGCT
FokI 18 CATCC GGATG(9/13)
1147 CATCC
HaeII 341 AGCGCT [GA]GCGC^[CT]
1019 AGCGCT
HinfI 639 GAATC G^A[ACGT]TC
1046 GAATC
Ksp632I 788 GAAGAG CTCTTC(1/4)
925 GAAGAG
MaeI 224 CTAG C^TAG
565 CTAG
MaeII 950 ACGT A^CGT
986 ACGT
MluNI 290 TGGCCA TGG^CCA
401 TGGCCA
MscI 290 TGGCCA TGG^CCA
401 TGGCCA
MslI 57 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
801 CATGCCCATG
NheI 223 GCTAGC G^CTAGC
564 GCTAGC
PpuMI 965 AGGACCC [GA]G^G[AT]CC[CT]
1091 AGGTCCT
SexAI 170 ACCAGGT A^CC[AT]GGT
836 ACCAGGT
SfcI 73 CTGCAG C^T[GA][CT]AG
243 CTACAG
SmaI 509 CCCGGG CCC^GGG
583 CCCGGG
StuI 714 AGGCCT AGG^CCT
1055 AGGCCT
TfiI 639 GAATC G^A[AT]TC
1046 GAATC
TspRI 433 CACTG CA[GC]TG[ACGT][ACGT]^
745 CAGTG
XmaCI 509 CCCGGG C^CCGGG
583 CCCGGG
XmaI 509 CCCGGG C^CCGGG
583 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanI BbvI BcnI BmyI
BsaJI BsgI BsiYI BslI Bsp1286I
BspMI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PvuII RsaI Sau3AI Sau96I
ScrFI StyI TaqI TseI TthHB8I