Restriction map for
pFN21AE2134
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AviII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BssHII BstAPI BstEII BstPI BstUI
CfoI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KspI MaeII MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SwaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1338 GGTACC G^GTACC
AccI 240 GTATAC GT^[AC][GT]AC
AciI 2217 CCGC CCGC(-3/-1)
Asp718I 1338 GGTACC G^GTACC
AspI 2197 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 24 CCTAGG C^CTAGG
BalI 854 TGGCCA TGG^CCA
BbsI 898 GTCTTC GAAGAC(2/6)
BlnI 24 CCTAGG C^CTAGG
BlpI 156 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 156 GCTGAGC GC^T[ACGT]AGC
BpuAI 898 GTCTTC GAAGAC(2/6)
BseRI 1380 CTCCTC GAGGAG(10/8)
BsgI 2119 GTGCAG GTGCAG(16/14)
BsmI 2124 GAATGC GAATGC(1/-1)
BspHI 1961 TCATGA T^CATGA
BsrFI 43 GCCGGT [GA]^CCGG[CT]
BssSI 914 CACGAG CACGAG(-5/-1)
Bst1107I 240 GTATAC GTA^TAC
BstBI 1059 TTCGAA TT^CGAA
BstXI 2466 CCAGGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 240 GTATAC GTA^TAC
Cac8I 847 GCTGGC GC[ACGT]^[ACGT]GC
CelII 156 GCTGAGC GC^T[ACGT]AGC
Cfr10I 43 GCCGGT [GA]^CCGG[CT]
DraIII 1709 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1874 CCACGG C^C[GA][CT]GG
EcoNI 111 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1219 GATATC GAT^ATC
HphI 113 TCACC GGTGA(8/7)
KpnI 1338 GGTACC GGTAC^C
MluNI 854 TGGCCA TGG^CCA
MscI 854 TGGCCA TGG^CCA
NspV 1059 TTCGAA TT^CGAA
PaeR7I 568 CTCGAG C^TCGAG
PflFI 2197 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1961 TCATGA T^CATGA
SfuI 1059 TTCGAA TT^CGAA
SpeI 1242 ACTAGT A^CTAGT
SspI 543 AATATT AAT^ATT
Tth111I 2197 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1311 TCTAGA T^CTAGA
XhoI 568 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 1078 CCTAAGG CC^T[ACGT]AGG
2455 CCTGAGG
Asp700I 59 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
660 GAACCCATTC
AvaI 229 CTCGGG C^[CT]CG[GA]G
568 CTCGAG
BcnI 800 CCGGG CC^[GC]GG
1989 CCGGG
BsaI 5 GAGACC GGTCTC(1/5)
388 GGTCTC
BsoBI 229 CTCGGG C^[CT]CG[GA]G
568 CTCGAG
Bsp1407I 2069 TGTACA T^GTACA
2427 TGTACA
BsrGI 2069 TGTACA T^GTACA
2427 TGTACA
Bsu36I 1078 CCTAAGG CC^T[ACGT]AGG
2455 CCTGAGG
DraI 429 TTTAAA TTT^AAA
1362 TTTAAA
EarI 1861 CTCTTC CTCTTC(1/4)
1891 GAAGAG
Eco81I 1078 CCTAAGG CC^T[ACGT]AGG
2455 CCTGAGG
EcoRI 59 GAATTC G^AATTC
507 GAATTC
Ksp632I 1861 CTCTTC CTCTTC(1/4)
1891 GAAGAG
MspA1I 211 CAGCTG C[AC]G^C[GT]G
217 CAGCTG
NciI 800 CCGGG CC^[GC]GG
1989 CCGGG
PflMI 786 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2022 CCAGAGACTGG
PstI 214 CTGCAG CTGCA^G
1814 CTGCAG
PvuII 211 CAGCTG CAG^CTG
217 CAGCTG
SspBI 2069 TGTACA T^GTACA
2427 TGTACA
StuI 266 AGGCCT AGG^CCT
761 AGGCCT
TaqI 569 TCGA T^CGA
1060 TCGA
TthHB8I 569 TCGA T^CGA
1060 TCGA
Van91I 786 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2022 CCAGAGACTGG
XmnI 59 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
660 GAACCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AspHI AvaII BanI BanII
BbvI BfaI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PpuMI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII