Restriction map for
pFN21AE9443
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII Acc65I AccII AclI AcyI
AgeI AhdI AscI Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BglI BlnI BsaHI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoO65I EcoT22I FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MluI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI XbaI XcmI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 4149 TCCGGA T^CCGGA
AflII 1816 CTTAAG C^TTAAG
Alw44I 3802 GTGCAC G^TGCAC
Aor51HI 181 AGCGCT AGC^GCT
ApaI 1542 GGGCCC GGGCC^C
ApaLI 3802 GTGCAC G^TGCAC
AseI 3172 ATTAAT AT^TAAT
AsnI 3172 ATTAAT AT^TAAT
Asp700I 2530 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3752 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 4245 TGGCCA TGG^CCA
BanI 3972 GGCACC G^G[CT][GA]CC
BbvCI 3985 CCTCAGC CCTCAGC(-5/-2)
BcgI 918 CGACAAACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1816 CTTAAG C^TTAAG
BglII 141 AGATCT A^GATCT
BlpI 1231 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1231 GCTGAGC GC^T[ACGT]AGC
BsaAI 1132 TACGTG [CT]AC^GT[GA]
BsaBI 1105 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4149 TCCGGA T^CCGGA
BspEI 4149 TCCGGA T^CCGGA
BspMI 3083 GCAGGT ACCTGC(4/8)
CelII 1231 GCTGAGC GC^T[ACGT]AGC
DraI 2050 TTTAAA TTT^AAA
DrdI 4045 GACAGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 4245 TGGCCA [CT]^GGCC[GA]
Eco47III 181 AGCGCT AGC^GCT
EcoNI 4187 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1884 GAATTC G^AATTC
EcoRV 787 GATATC GAT^ATC
FauI 466 GCGGG CCCGC(4/6)
MamI 1105 GATTACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4245 TGGCCA TGG^CCA
MroI 4149 TCCGGA T^CCGGA
MscI 4245 TGGCCA TGG^CCA
NcoI 3659 CCATGG C^CATGG
NdeI 1879 CATATG CA^TATG
PflFI 3752 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3172 ATTAAT AT^TAAT
SapI 3124 GCTCTTC GCTCTTC(1/4)
SexAI 3148 ACCTGGT A^CC[AT]GGT
SmaI 153 CCCGGG CCC^GGG
StuI 3318 AGGCCT AGG^CCT
Tth111I 3752 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 153 CCCGGG C^CCGGG
XmaI 153 CCCGGG C^CCGGG
XmnI 2530 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 1133 ACGTGT A^C[GA][CT]GT
2452 ACGTGT
AocI 1477 CCTAAGG CC^T[ACGT]AGG
1537 CCTAAGG
BciVI 3648 GGATAC GTATCC(6/5)
3663 GGATAC
BclI 3029 TGATCA T^GATCA
3785 TGATCA
BcnI 153 CCCGG CC^[GC]GG
154 CCGGG
BsaI 1851 GAGACC GGTCTC(1/5)
1980 GGTCTC
BsaWI 2159 ACCGGA [AT]^CCGG[AT]
4149 TCCGGA
BsgI 3706 CTGCAC GTGCAG(16/14)
2797 GTGCAG
BsmI 20 GAATGC GAATGC(1/-1)
3054 GCATTC
BspHI 170 TCATGA T^CATGA
2526 TCATGA
Bst1107I 913 GTATAC GTA^TAC
2293 GTATAC
BstYI 141 AGATCT [GA]^GATC[CT]
2039 AGATCC
BstZ17I 913 GTATAC GTA^TAC
2293 GTATAC
Bsu36I 1477 CCTAAGG CC^T[ACGT]AGG
1537 CCTAAGG
CfoI 182 GCGC GCG^C
3116 GCGC
DraIII 1570 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3033 CACATTGTG
Eco81I 1477 CCTAAGG CC^T[ACGT]AGG
1537 CCTAAGG
EcoT14I 1421 CCAAGG C^C[AT][AT]GG
3659 CCATGG
FbaI 3029 TGATCA T^GATCA
3785 TGATCA
HaeII 181 AGCGCT [GA]GCGC^[CT]
3115 GGCGCT
HhaI 182 GCGC GCG^C
3116 GCGC
HinP1I 182 GCGC G^CGC
3116 GCGC
HincII 1828 GTTGAC GT[CT]^[GA]AC
2428 GTTGAC
HindII 1828 GTTGAC GT[CT]^[GA]AC
2428 GTTGAC
HindIII 1859 AAGCTT A^AGCTT
2939 AAGCTT
MflI 141 AGATCT [GA]^GATC[CT]
2039 AGATCC
NciI 153 CCCGG CC^[GC]GG
154 CCGGG
PaeR7I 3841 CTCGAG C^TCGAG
3967 CTCGAG
PflMI 343 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4231 CCAAATCTTGG
PpuMI 412 GGGACCT [GA]G^G[AT]CC[CT]
482 AGGACCC
RcaI 170 TCATGA T^CATGA
2526 TCATGA
SacI 3844 GAGCTC GAGCT^C
3949 GAGCTC
StyI 1421 CCAAGG C^C[AT][AT]GG
3659 CCATGG
Van91I 343 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4231 CCAAATCTTGG
XhoI 3841 CTCGAG C^TCGAG
3967 CTCGAG
XhoII 141 AGATCT [GA]^GATC[CT]
2039 AGATCC
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MnlI MseI
MslI MspA1I MspI MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I