Restriction map for
pFN21AE9284
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglI
BlnI BpuAI BsaBI BsaHI BsaI
BseAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI Bsu36I Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SwaI
TfiI Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AfaI 720 GTAC GT^AC
Asp700I 918 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 805 GTGCTC G[AT]GC[AT]^C
BalI 569 TGGCCA TGG^CCA
BamHI 164 GGATCC G^GATCC
BanI 877 GGCACC G^G[CT][GA]CC
BclI 840 TGATCA T^GATCA
BcnI 801 CCCGG CC^[GC]GG
BglII 483 AGATCT A^GATCT
BpmI 355 CTGGAG CTGGAG(16/14)
BsaAI 604 CACGTA [CT]AC^GT[GA]
BsaWI 40 ACCGGA [AT]^CCGG[AT]
BseRI 325 GAGGAG GAGGAG(10/8)
BsiEI 939 CGACCG CG[GA][CT]^CG
BsiHKAI 805 GTGCTC G[AT]GC[AT]^C
BsmAI 797 GTCTC GTCTC(1/5)
BsmI 962 GAATGC GAATGC(1/-1)
BspHI 759 TCATGA T^CATGA
Bst1107I 372 GTATAC GTA^TAC
BstNI 180 CCAGG CC^[AT]GG
BstXI 83 CCAGCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 372 GTATAC GTA^TAC
DraII 746 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 274 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 403 GACATCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 278 CCGTGG C^C[GA][CT]GG
EaeI 569 TGGCCA [CT]^GGCC[GA]
EarI 283 GAAGAG CTCTTC(1/4)
EcoNI 768 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 746 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 180 CCAGG ^CC[AT]GG
EcoT14I 320 CCAAGG C^C[AT][AT]GG
FbaI 840 TGATCA T^GATCA
HaeII 676 AGCGCC [GA]GCGC^[CT]
HgaI 701 GCGTC GACGC(5/10)
HinfI 505 GAGTC G^A[ACGT]TC
Ksp632I 283 GAAGAG CTCTTC(1/4)
MluNI 569 TGGCCA TGG^CCA
MscI 569 TGGCCA TGG^CCA
MseI 755 TTAA T^TAA
MslI 681 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 51 CAGCGG C[AC]G^C[GT]G
MvaI 180 CCAGG CC^[AT]GG
NciI 801 CCCGG CC^[GC]GG
NspI 170 GCATGC [GA]CATG^[CT]
PflMI 273 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 505 GAGTC GAGTC(4/5)
PpuMI 746 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 23 CTGCAG CTGCA^G
RcaI 759 TCATGA T^CATGA
RsaI 720 GTAC GT^AC
SfcI 23 CTGCAG C^T[GA][CT]AG
SphI 170 GCATGC GCATG^C
SspI 308 AATATT AAT^ATT
StyI 320 CCAAGG C^C[AT][AT]GG
Tru9I 755 TTAA T^TAA
Van91I 273 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 918 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 372 GTATAC GT^[AC][GT]AC
598 GTAGAC
AccII 700 CGCG CG^CG
967 CGCG
AvaII 38 GGACC G^G[AT]CC
747 GGTCC
BlpI 550 GCTGAGC GC^T[ACGT]AGC
807 GCTCAGC
Bpu1102I 550 GCTGAGC GC^T[ACGT]AGC
807 GCTCAGC
BsgI 691 CTGCAC GTGCAG(16/14)
196 GTGCAG
BspMI 198 GCAGGT ACCTGC(4/8)
420 GCAGGT
BssKI 180 CCAGG ^CC[ACGT]GG
801 CCCGG
BstUI 700 CGCG CG^CG
967 CGCG
CelII 550 GCTGAGC GC^T[ACGT]AGC
807 GCTCAGC
HapII 41 CCGG C^CGG
802 CCGG
HpaII 41 CCGG C^CGG
802 CCGG
MaeIII 103 GTGAC ^GT[ACGT]AC
791 GTCAC
MspI 41 CCGG C^CGG
802 CCGG
MvnI 700 CGCG CG^CG
967 CGCG
ScrFI 180 CCAGG CC^[ACGT]GG
801 CCCGG
StuI 728 AGGCCT AGG^CCT
776 AGGCCT
TaqI 242 TCGA T^CGA
437 TCGA
Tsp45I 103 GTGAC ^GT[GC]AC
791 GTCAC
TthHB8I 242 TCGA T^CGA
437 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BstF5I BstYI Cac8I
CfoI Cfr13I DdeI DpnII FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HphI ItaI MaeII MboI MboII
MflI MnlI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I SfaNI TseI
TspRI XhoII