Restriction map for
pFN21AE3544
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI MaeI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SfiI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 1150 GAATTC [GA]^AATT[CT]
Alw44I 346 GTGCAC G^TGCAC
AlwNI 998 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 346 GTGCAC G^TGCAC
ApoI 1150 GAATTC [GA]^AATT[CT]
AviII 470 TGCGCA TGC^GCA
BamHI 59 GGATCC G^GATCC
BbsI 28 GTCTTC GAAGAC(2/6)
BglI 1006 GCCGGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 333 CTCCAG CTGGAG(16/14)
BpuAI 28 GTCTTC GAAGAC(2/6)
BsaI 892 GAGACC GGTCTC(1/5)
BsgI 328 CTGCAC GTGCAG(16/14)
BsiEI 35 CGGCCG CG[GA][CT]^CG
BsmBI 463 GAGACG CGTCTC(1/5)
Bsp1407I 701 TGTACA T^GTACA
BsrBI 22 GAGCGG CCGCTC(-3/-3)
BsrDI 159 CATTGC GCAATG(2/0)
BsrGI 701 TGTACA T^GTACA
BstBI 169 TTCGAA TT^CGAA
EagI 35 CGGCCG C^GGCCG
EarI 210 GAAGAG CTCTTC(1/4)
EclXI 35 CGGCCG C^GGCCG
Eco52I 35 CGGCCG C^GGCCG
EcoNI 237 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1150 GAATTC G^AATTC
FspI 470 TGCGCA TGC^GCA
Ksp632I 210 GAAGAG CTCTTC(1/4)
KspI 496 CCGCGG CCGC^GG
NaeI 1006 GCCGGC GCC^GGC
NgoMIV 1006 GCCGGC G^CCGGC
NlaIII 678 CATG CATG^
NspV 169 TTCGAA TT^CGAA
PpuMI 216 GGGACCC [GA]G^G[AT]CC[CT]
PstI 11 CTGCAG CTGCA^G
SacII 496 CCGCGG CCGC^GG
SexAI 83 ACCTGGT A^CC[AT]GGT
SfcI 11 CTGCAG C^T[GA][CT]AG
SfuI 169 TTCGAA TT^CGAA
SgrAI 1038 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 762 CCCGGG CCC^GGG
SmlI 355 CTCAAG C^T[CT][GA]AG
SspBI 701 TGTACA T^GTACA
StuI 752 AGGCCT AGG^CCT
TfiI 40 GAATC G^A[AT]TC
Tsp509I 1151 AATT ^AATT
XmaCI 762 CCCGGG C^CCGGG
XmaI 762 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AocI 988 CCTGAGG CC^T[ACGT]AGG
1162 CCTGAGG
AspHI 346 GTGCAC G[AT]GC[AT]^C
934 GTGCTC
AvaII 217 GGACC G^G[AT]CC
411 GGACC
BbvCI 755 CCTCAGC CCTCAGC(-5/-2)
748 GCTGAGG
BsiHKAI 346 GTGCAC G[AT]GC[AT]^C
934 GTGCTC
BsmAI 463 GAGAC GTCTC(1/5)
892 GAGAC
BsrI 1070 ACTGG ACTGG(1/-1)
308 CCAGT
BstYI 59 GGATCC [GA]^GATC[CT]
668 AGATCC
Bsu36I 988 CCTGAGG CC^T[ACGT]AGG
1162 CCTGAGG
DsaI 496 CCGCGG C^C[GA][CT]GG
1022 CCACGG
EaeI 35 CGGCCG [CT]^GGCC[GA]
782 TGGCCG
Eco81I 988 CCTGAGG CC^T[ACGT]AGG
1162 CCTGAGG
MboII 210 GAAGA GAAGA(8/7)
29 TCTTC
MflI 59 GGATCC [GA]^GATC[CT]
668 AGATCC
PleI 148 GACTC GAGTC(4/5)
958 GAGTC
PvuII 915 CAGCTG CAG^CTG
998 CAGCTG
XhoII 59 GGATCC [GA]^GATC[CT]
668 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI BanI BanII
BbeI BbvI BcnI BmyI BsaHI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeIII MboI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI TaqI
TseI Tsp45I TspRI TthHB8I