Restriction map for
pFN21AE6076
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcsI 192 GAATTT [GA]^AATT[CT]
AfaI 177 GTAC GT^AC
ApaI 680 GGGCCC GGGCC^C
ApoI 192 GAATTT [GA]^AATT[CT]
Asp700I 855 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 139 GTGCTC G[AT]GC[AT]^C
AvaII 465 GGTCC G^G[AT]CC
BpmI 205 CTGGAG CTGGAG(16/14)
BsaI 397 GAGACC GGTCTC(1/5)
BsiHKAI 139 GTGCTC G[AT]GC[AT]^C
BsmFI 569 GGGAC GGGAC(10/14)
BsmI 659 GCATTC GAATGC(1/-1)
BspMI 578 ACCTGC ACCTGC(4/8)
BsrDI 878 GCAATG GCAATG(2/0)
BstEII 96 GGTGACC G^GT[ACGT]ACC
BstPI 96 GGTGACC G^GT[ACGT]ACC
CfoI 722 GCGC GCG^C
EcoNI 548 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 96 GGTGACC G^GT[ACGT]ACC
EcoT14I 911 CCTTGG C^C[AT][AT]GG
HhaI 722 GCGC GCG^C
HinP1I 722 GCGC G^CGC
HindIII 30 AAGCTT A^AGCTT
HphI 96 GGTGA GGTGA(8/7)
MaeII 610 ACGT A^CGT
MseI 91 TTAA T^TAA
MwoI 682 GCCCCTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 447 GCTAGC G^CTAGC
PleI 18 GACTC GAGTC(4/5)
PpuMI 464 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 177 GTAC GT^AC
SfcI 686 CTACAG C^T[GA][CT]AG
SpeI 429 ACTAGT A^CTAGT
StyI 911 CCTTGG C^C[AT][AT]GG
TfiI 455 GATTC G^A[AT]TC
Tru9I 91 TTAA T^TAA
XcmI 476 CCACTGTCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 855 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 264 GGATC GGATC(4/5)
360 GGATC
AlwNI 70 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
689 CAGCTGCTG
BalI 411 TGGCCA TGG^CCA
488 TGGCCA
BanI 322 GGCACC G^G[CT][GA]CC
817 GGTGCC
BanII 624 GGGCTC G[GA]GC[CT]^C
680 GGGCCC
BlpI 141 GCTCAGC GC^T[ACGT]AGC
694 GCTGAGC
Bpu1102I 141 GCTCAGC GC^T[ACGT]AGC
694 GCTGAGC
BsgI 163 CTGCAC GTGCAG(16/14)
889 GTGCAG
BsmAI 397 GAGAC GTCTC(1/5)
354 GTCTC
BstF5I 252 CATCC GGATG(2/0)
294 CATCC
CelII 141 GCTCAGC GC^T[ACGT]AGC
694 GCTGAGC
DpnII 265 GATC ^GATC
361 GATC
FokI 252 CATCC GGATG(9/13)
294 CATCC
HinfI 18 GACTC G^A[ACGT]TC
455 GATTC
MboI 265 GATC ^GATC
361 GATC
MluNI 411 TGGCCA TGG^CCA
488 TGGCCA
MscI 411 TGGCCA TGG^CCA
488 TGGCCA
NdeII 265 GATC ^GATC
361 GATC
Sau3AI 265 GATC ^GATC
361 GATC
Tsp509I 132 AATT ^AATT
193 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI BbvI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DraII EaeI EcoO109I EcoRII Fnu4HI
HaeIII ItaI MaeI MaeIII MboII
MnlI MslI MspA1I MvaI NlaIII
NlaIV PvuII Sau96I ScrFI SfaNI
TseI Tsp45I TspRI