Restriction map for
pFN21AE6063
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AlwI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbsI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
Cfr10I CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MamI MflI MluI MroI
MseI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tth111I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Alw44I 160 GTGCAC G^TGCAC
AlwNI 241 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 876 CCTGAGG CC^T[ACGT]AGG
ApaLI 160 GTGCAC G^TGCAC
AvaI 668 CCCGGG C^[CT]CG[GA]G
BbrPI 733 CACGTG CAC^GTG
BbvI 373 GCTGC GCAGC(8/12)
BciVI 573 GGATAC GTATCC(6/5)
BglI 630 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 733 CACGTG [CT]AC^GT[GA]
BsmI 712 GCATTC GAATGC(1/-1)
BsoBI 668 CCCGGG C^[CT]CG[GA]G
BspDI 114 ATCGAT AT^CGAT
BssSI 225 CACGAG CACGAG(-5/-1)
Bsu36I 876 CCTGAGG CC^T[ACGT]AGG
ClaI 114 ATCGAT AT^CGAT
DdeI 877 CTGAG C^T[ACGT]AG
Eco81I 876 CCTGAGG CC^T[ACGT]AGG
EcoT14I 426 CCTTGG C^C[AT][AT]GG
EcoT22I 409 ATGCAT ATGCA^T
Fnu4HI 373 GCTGC GC^[ACGT]GC
HaeII 898 AGCGCC [GA]GCGC^[CT]
ItaI 373 GCTGC GC^[ACGT]GC
MaeII 734 ACGT A^CGT
MaeIII 844 GTCAC ^GT[ACGT]AC
MfeI 516 CAATTG C^AATTG
MspA1I 502 CCGCTG C[AC]G^C[GT]G
MunI 516 CAATTG C^AATTG
NheI 432 GCTAGC G^CTAGC
NsiI 409 ATGCAT ATGCA^T
PflMI 315 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 733 CACGTG CAC^GTG
PmlI 733 CACGTG CAC^GTG
SapI 894 GAAGAGC GCTCTTC(1/4)
SmaI 668 CCCGGG CCC^GGG
SmlI 96 CTTGAG C^T[CT][GA]AG
StyI 426 CCTTGG C^C[AT][AT]GG
TseI 373 GCTGC G^C[AT]GC
Tsp45I 844 GTCAC ^GT[GC]AC
TspRI 399 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 315 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 464 CCACCAGCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 668 CCCGGG C^CCGGG
XmaI 668 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AfaI 180 GTAC GT^AC
600 GTAC
AspHI 160 GTGCAC G[AT]GC[AT]^C
795 GAGCAC
AvaII 262 GGACC G^G[AT]CC
297 GGTCC
BalI 347 TGGCCA TGG^CCA
605 TGGCCA
BanII 477 GGGCTC G[GA]GC[CT]^C
637 GGGCTC
BcnI 668 CCCGG CC^[GC]GG
669 CCGGG
BfaI 70 CTAG C^TAG
433 CTAG
BpmI 26 CTCCAG CTGGAG(16/14)
781 CTCCAG
BsaI 144 GGTCTC GGTCTC(1/5)
648 GGTCTC
BsiHKAI 160 GTGCAC G[AT]GC[AT]^C
795 GAGCAC
BsmFI 261 GGGAC GGGAC(10/14)
235 GTCCC
BsrDI 663 CATTGC GCAATG(2/0)
125 GCAATG
BsrI 561 ACTGG ACTGG(1/-1)
300 CCAGT
BstF5I 412 CATCC GGATG(2/0)
642 CATCC
BstXI 464 CCACCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
467 CCAGCATGGTGG
DpnII 117 GATC ^GATC
684 GATC
EarI 82 CTCTTC CTCTTC(1/4)
894 GAAGAG
FokI 412 CATCC GGATG(9/13)
642 CATCC
HapII 669 CCGG C^CGG
906 CCGG
HpaII 669 CCGG C^CGG
906 CCGG
Ksp632I 82 CTCTTC CTCTTC(1/4)
894 GAAGAG
MaeI 70 CTAG C^TAG
433 CTAG
MboI 117 GATC ^GATC
684 GATC
MluNI 347 TGGCCA TGG^CCA
605 TGGCCA
MscI 347 TGGCCA TGG^CCA
605 TGGCCA
MspI 669 CCGG C^CGG
906 CCGG
NciI 668 CCCGG CC^[GC]GG
669 CCGGG
NdeII 117 GATC ^GATC
684 GATC
NlaIV 261 GGGACC GG[ACGT]^[ACGT]CC
478 GGCTCC
PstI 381 CTGCAG CTGCA^G
938 CTGCAG
RsaI 180 GTAC GT^AC
600 GTAC
Sau3AI 117 GATC ^GATC
684 GATC
SfcI 381 CTGCAG C^T[GA][CT]AG
938 CTGCAG
TaqI 115 TCGA T^CGA
524 TCGA
Tsp509I 517 AATT ^AATT
957 AATT
TthHB8I 115 TCGA T^CGA
524 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BssKI BstNI
Cac8I CfoI Cfr13I EaeI EcoRII
HaeIII HhaI HinP1I HphI MboII
MnlI MslI MvaI MwoI NlaIII
Sau96I ScrFI SfaNI