Restriction map for
pFN21AE6058
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BsrBI BssHII BssSI Bst1107I BstBI
BstUI BstYI BstZ17I Bsu36I CfoI
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MroI MseI
MspA1I MunI MvnI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI Tru9I Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 395 GGCGTC G[GA]^CG[CT]C
AflIII 733 ACATGT A^C[GA][CT]GT
AvaI 704 CCCGGG C^[CT]CG[GA]G
AvaII 309 GGTCC G^G[AT]CC
BfaI 73 CTAG C^TAG
BglI 205 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 230 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 230 GCTTAGC GC^T[ACGT]AGC
BsaHI 395 GGCGTC G[GA]^CG[CT]C
BsmFI 820 GTCCC GGGAC(10/14)
BsoBI 704 CCCGGG C^[CT]CG[GA]G
Bsp1407I 324 TGTACA T^GTACA
BspLU11I 733 ACATGT A^CATGT
BspMI 870 ACCTGC ACCTGC(4/8)
BsrFI 150 GCCGGC [GA]^CCGG[CT]
BsrGI 324 TGTACA T^GTACA
BstAPI 331 GCACTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 420 GGTCACC G^GT[ACGT]ACC
BstPI 420 GGTCACC G^GT[ACGT]ACC
BstXI 218 CCACAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 230 GCTTAGC GC^T[ACGT]AGC
Cfr10I 150 GCCGGC [GA]^CCGG[CT]
EarI 781 CTCTTC CTCTTC(1/4)
EcoO65I 420 GGTCACC G^GT[ACGT]ACC
FauI 899 CCCGC CCCGC(4/6)
HgaI 396 GCGTC GACGC(5/10)
Hin1I 395 GGCGTC G[GA]^CG[CT]C
HphI 422 TCACC GGTGA(8/7)
Ksp632I 781 CTCTTC CTCTTC(1/4)
MaeI 73 CTAG C^TAG
NaeI 150 GCCGGC GCC^GGC
NdeI 293 CATATG CA^TATG
NgoMIV 150 GCCGGC G^CCGGC
PshAI 96 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 691 CTGCAG CTGCA^G
SexAI 774 ACCTGGT A^CC[AT]GGT
SmaI 704 CCCGGG CCC^GGG
SspBI 324 TGTACA T^GTACA
TfiI 346 GAATC G^A[AT]TC
Tsp45I 421 GTCAC ^GT[GC]AC
Tsp509I 22 AATT ^AATT
XmaCI 704 CCCGGG C^CCGGG
XmaI 704 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AluI 468 AGCT AG^CT
695 AGCT
Alw44I 551 GTGCAC G^TGCAC
832 GTGCAC
ApaLI 551 GTGCAC G^TGCAC
832 GTGCAC
BcnI 704 CCCGG CC^[GC]GG
705 CCGGG
BsaI 917 GAGACC GGTCTC(1/5)
778 GGTCTC
BsgI 178 CTGCAC GTGCAG(16/14)
403 CTGCAC
BsmAI 917 GAGAC GTCTC(1/5)
779 GTCTC
BsrDI 675 CATTGC GCAATG(2/0)
137 GCAATG
BstF5I 207 CATCC GGATG(2/0)
837 CATCC
DdeI 231 CTTAG C^T[ACGT]AG
633 CTCAG
DraII 277 GGGCCCC [GA]G^G[ACGT]CC[CT]
895 GGGGCCC
DsaI 341 CCATGG C^C[GA][CT]GG
365 CCATGG
EcoO109I 277 GGGCCCC [GA]G^G[ACGT]CC[CT]
895 GGGGCCC
FokI 207 CATCC GGATG(9/13)
837 CATCC
HapII 151 CCGG C^CGG
705 CCGG
HpaII 151 CCGG C^CGG
705 CCGG
MaeIII 421 GTCAC ^GT[ACGT]AC
584 GTAAC
MboII 47 TCTTC GAAGA(8/7)
782 TCTTC
MslI 558 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
745 CACATCTGTG
MspI 151 CCGG C^CGG
705 CCGG
NciI 704 CCCGG CC^[GC]GG
705 CCGGG
NcoI 341 CCATGG C^CATGG
365 CCATGG
NspI 327 ACATGC [GA]CATG^[CT]
733 ACATGT
SfcI 242 CTATAG C^T[GA][CT]AG
691 CTGCAG
StuI 722 AGGCCT AGG^CCT
761 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI ApaI AspHI BalI
BanII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DpnII EaeI
EcoRII EcoT14I Fnu4HI HaeIII HinfI
ItaI MboI MluNI MnlI MscI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
StyI TseI TspRI XcmI