Restriction map for
pFN21AE6056
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MfeI
MluI MroI MspA1I MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI TfiI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AciI 795 CCGC CCGC(-3/-1)
AgeI 362 ACCGGT A^CCGGT
Asp700I 31 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 118 CTCGGG C^[CT]CG[GA]G
AvrII 765 CCTAGG C^CTAGG
BamHI 638 GGATCC G^GATCC
BanII 896 GGGCTC G[GA]GC[CT]^C
BlnI 765 CCTAGG C^CTAGG
BsaBI 246 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 765 CCTAGG C^C[ACGT][ACGT]GG
BsaWI 362 ACCGGT [AT]^CCGG[AT]
BsmAI 742 GTCTC GTCTC(1/5)
BsmFI 626 GTCCC GGGAC(10/14)
BsoBI 118 CTCGGG C^[CT]CG[GA]G
Bsp1407I 830 TGTACA T^GTACA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrFI 362 ACCGGT [GA]^CCGG[CT]
BsrGI 830 TGTACA T^GTACA
BsrI 634 ACTGG ACTGG(1/-1)
BstEII 840 GGTCACC G^GT[ACGT]ACC
BstPI 840 GGTCACC G^GT[ACGT]ACC
BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 638 GGATCC [GA]^GATC[CT]
Cac8I 286 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 610 GCGC GCG^C
Cfr10I 362 ACCGGT [GA]^CCGG[CT]
DpnII 639 GATC ^GATC
DraII 624 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 586 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 624 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 840 GGTCACC G^GT[ACGT]ACC
EcoT14I 765 CCTAGG C^C[AT][AT]GG
FauI 794 CCCGC CCCGC(4/6)
HapII 363 CCGG C^CGG
HhaI 610 GCGC GCG^C
HinP1I 610 GCGC G^CGC
HinfI 154 GACTC G^A[ACGT]TC
HpaII 363 CCGG C^CGG
MamI 246 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 639 GATC ^GATC
MflI 638 GGATCC [GA]^GATC[CT]
MspI 363 CCGG C^CGG
NdeII 639 GATC ^GATC
NspI 675 ACATGC [GA]CATG^[CT]
PinAI 362 ACCGGT A^CCGGT
PleI 154 GACTC GAGTC(4/5)
PpuMI 624 AGGTCCC [GA]G^G[AT]CC[CT]
Sau3AI 639 GATC ^GATC
SexAI 104 ACCTGGT A^CC[AT]GGT
SfcI 867 CTATAG C^T[GA][CT]AG
SmlI 807 CTCAAG C^T[CT][GA]AG
SspBI 830 TGTACA T^GTACA
StyI 765 CCTAGG C^C[AT][AT]GG
XhoII 638 GGATCC [GA]^GATC[CT]
XmnI 31 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 23 AAATTT [GA]^AATT[CT]
31 GAATTT
AluI 574 AGCT AG^CT
690 AGCT
AlwI 639 GATCC GGATC(4/5)
638 GGATC
ApoI 23 AAATTT [GA]^AATT[CT]
31 GAATTT
AspHI 115 GTGCTC G[AT]GC[AT]^C
455 GTGCTC
AvaII 446 GGACC G^G[AT]CC
625 GGTCC
BalI 140 TGGCCA TGG^CCA
371 TGGCCA
BbvI 612 GCTGC GCAGC(8/12)
787 GCTGC
BfaI 436 CTAG C^TAG
766 CTAG
BsgI 388 CTGCAC GTGCAG(16/14)
504 CTGCAC
BsiHKAI 115 GTGCTC G[AT]GC[AT]^C
455 GTGCTC
Cfr13I 446 GGACC G^G[ACGT]CC
625 GGTCC
EaeI 140 TGGCCA [CT]^GGCC[GA]
371 TGGCCA
Fnu4HI 612 GCTGC GC^[ACGT]GC
787 GCTGC
ItaI 612 GCTGC GC^[ACGT]GC
787 GCTGC
MaeI 436 CTAG C^TAG
766 CTAG
MluNI 140 TGGCCA TGG^CCA
371 TGGCCA
MscI 140 TGGCCA TGG^CCA
371 TGGCCA
Sau96I 446 GGACC G^G[ACGT]CC
625 GGTCC
SfaNI 439 GCATC GCATC(5/9)
724 GCATC
TseI 612 GCTGC G^C[AT]GC
787 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AfaI BmyI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI DdeI EcoRII
FokI HaeIII HphI MaeIII MboII
MnlI MseI MslI MvaI NlaIII
NlaIV RsaI ScrFI Tru9I Tsp45I
Tsp509I TspRI