Restriction map for
pFN21AE6038
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BsrBI
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MaeI MamI MluI
MroI MseI MvnI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 826 AAATTT [GA]^AATT[CT]
AfaI 189 GTAC GT^AC
ApoI 826 AAATTT [GA]^AATT[CT]
BanII 908 GGGCTC G[GA]GC[CT]^C
BcgI 473 GCAACTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 563 TGATCA T^GATCA
BglI 358 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 936 CTGGAG CTGGAG(16/14)
BsaAI 379 TACGTG [CT]AC^GT[GA]
BseRI 915 GAGGAG GAGGAG(10/8)
BsiEI 557 CGGCCG CG[GA][CT]^CG
BsmBI 753 CGTCTC CGTCTC(1/5)
Bsp1407I 188 TGTACA T^GTACA
BspLU11I 782 ACATGT A^CATGT
BsrFI 555 GCCGGC [GA]^CCGG[CT]
BsrGI 188 TGTACA T^GTACA
BssSI 442 CTCGTG CACGAG(-5/-1)
BstAPI 338 GCACGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 555 GCCGGC [GA]^CCGG[CT]
DraIII 339 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 557 CGGCCG C^GGCCG
EclXI 557 CGGCCG C^GGCCG
Eco52I 557 CGGCCG C^GGCCG
FauI 518 GCGGG CCCGC(4/6)
FbaI 563 TGATCA T^GATCA
HincII 259 GTCAAC GT[CT]^[GA]AC
HindII 259 GTCAAC GT[CT]^[GA]AC
HinfI 31 GACTC G^A[ACGT]TC
MfeI 505 CAATTG C^AATTG
MslI 585 CACCGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 387 CAGCTG C[AC]G^C[GT]G
MunI 505 CAATTG C^AATTG
NaeI 555 GCCGGC GCC^GGC
NcoI 362 CCATGG C^CATGG
NgoMIV 555 GCCGGC G^CCGGC
NspI 782 ACATGT [GA]CATG^[CT]
PleI 31 GACTC GAGTC(4/5)
PstI 790 CTGCAG CTGCA^G
PvuII 387 CAGCTG CAG^CTG
RsaI 189 GTAC GT^AC
SfcI 790 CTGCAG C^T[GA][CT]AG
SfiI 357 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 188 TGTACA T^GTACA
StuI 719 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 730 ACGTGT A^C[GA][CT]GT
782 ACATGT
AspHI 425 GTGCTC G[AT]GC[AT]^C
685 GTGCTC
AvaI 158 CCCGGG C^[CT]CG[GA]G
677 CCCGGG
BamHI 165 GGATCC G^GATCC
692 GGATCC
BanI 552 GGTGCC G^G[CT][GA]CC
636 GGTGCC
BbsI 279 GAAGAC GAAGAC(2/6)
319 GTCTTC
BpuAI 279 GAAGAC GAAGAC(2/6)
319 GTCTTC
BsiHKAI 425 GTGCTC G[AT]GC[AT]^C
685 GTGCTC
BsmAI 918 GAGAC GTCTC(1/5)
754 GTCTC
BsoBI 158 CCCGGG C^[CT]CG[GA]G
677 CCCGGG
BstXI 143 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
362 CCATGGCCCTGG
DsaI 73 CCGTGG C^C[GA][CT]GG
362 CCATGG
EarI 760 CTCTTC CTCTTC(1/4)
838 CTCTTC
EcoT14I 362 CCATGG C^C[AT][AT]GG
704 CCAAGG
Ksp632I 760 CTCTTC CTCTTC(1/4)
838 CTCTTC
MaeIII 8 GTGAC ^GT[ACGT]AC
203 GTCAC
PflMI 324 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
929 CCAGGATCTGG
SmaI 158 CCCGGG CCC^GGG
677 CCCGGG
StyI 362 CCATGG C^C[AT][AT]GG
704 CCAAGG
Tsp45I 8 GTGAC ^GT[GC]AC
203 GTCAC
Tsp509I 506 AATT ^AATT
827 AATT
Van91I 324 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
929 CCAGGATCTGG
XmaCI 158 CCCGGG C^CCGGG
677 CCCGGG
XmaI 158 CCCGGG C^CCGGG
677 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BalI BbvI BcnI BmyI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI MaeII
MboI MboII MflI MluNI MnlI
MscI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI TspRI XhoII