Restriction map for
pFN21AE6031
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AgeI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DrdI DsaI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NarI NciI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 908 GTCGAC GT^[AC][GT]AC
AhdI 911 GACATGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 911 GACATGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 51 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 160 GGGCTC G[GA]GC[CT]^C
BbsI 598 GTCTTC GAAGAC(2/6)
BfaI 413 CTAG C^TAG
BpuAI 598 GTCTTC GAAGAC(2/6)
BseRI 323 GAGGAG GAGGAG(10/8)
BsgI 388 CTGCAC GTGCAG(16/14)
BsmAI 57 GTCTC GTCTC(1/5)
BspHI 404 TCATGA T^CATGA
BspMI 756 ACCTGC ACCTGC(4/8)
BsrDI 427 GCAATG GCAATG(2/0)
BsrFI 380 GCCGGC [GA]^CCGG[CT]
BssSI 725 CTCGTG CACGAG(-5/-1)
BstXI 236 CCAGGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 380 GCCGGC [GA]^CCGG[CT]
DraII 787 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 823 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 911 GACATGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 894 GAAGAG CTCTTC(1/4)
EcoO109I 787 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 209 ATGCAT ATGCA^T
HapII 381 CCGG C^CGG
HincII 908 GTCGAC GT[CT]^[GA]AC
HindII 908 GTCGAC GT[CT]^[GA]AC
HpaII 381 CCGG C^CGG
HphI 140 TCACC GGTGA(8/7)
Ksp632I 894 GAAGAG CTCTTC(1/4)
MaeI 413 CTAG C^TAG
MaeIII 25 GTGAC ^GT[ACGT]AC
MseI 94 TTAA T^TAA
MspI 381 CCGG C^CGG
NaeI 380 GCCGGC GCC^GGC
NgoMIV 380 GCCGGC G^CCGGC
NlaIV 231 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 209 ATGCAT ATGCA^T
PflFI 51 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 404 TCATGA T^CATGA
SalI 908 GTCGAC G^TCGAC
SfcI 279 CTACAG C^T[GA][CT]AG
SmlI 59 CTCAAG C^T[CT][GA]AG
TaqI 909 TCGA T^CGA
TfiI 846 GAATC G^A[AT]TC
Tru9I 94 TTAA T^TAA
Tsp45I 25 GTGAC ^GT[GC]AC
Tth111I 51 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 909 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
492 CCGC
AcsI 31 GAATTT [GA]^AATT[CT]
123 AAATTT
ApoI 31 GAATTT [GA]^AATT[CT]
123 AAATTT
AvaII 480 GGTCC G^G[AT]CC
754 GGACC
BbvI 682 GCAGC GCAGC(8/12)
287 GCTGC
BmyI 160 GGGCTC G[AGT]GC[ACT]^C
777 GTGCCC
Bsp1286I 160 GGGCTC G[AGT]GC[ACT]^C
777 GTGCCC
DpnII 90 GATC ^GATC
831 GATC
EcoT14I 42 CCTTGG C^C[AT][AT]GG
318 CCTTGG
Fnu4HI 287 GCTGC GC^[ACGT]GC
682 GCAGC
ItaI 287 GCTGC GC^[ACGT]GC
682 GCAGC
MboI 90 GATC ^GATC
831 GATC
MspA1I 517 CAGCTG C[AC]G^C[GT]G
797 CAGCTG
NdeII 90 GATC ^GATC
831 GATC
PleI 51 GACTC GAGTC(4/5)
906 GAGTC
PvuII 517 CAGCTG CAG^CTG
797 CAGCTG
Sau3AI 90 GATC ^GATC
831 GATC
StyI 42 CCTTGG C^C[AT][AT]GG
318 CCTTGG
TseI 287 GCTGC G^C[AT]GC
682 GCAGC
XcmI 236 CCAGGATGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
467 CCATTATCCAGGTGG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AflIII AluI AlwNI BsaJI BsiYI
BslI BspLU11I BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII FokI
HaeIII HinfI MboII MnlI MslI
MvaI MwoI NlaIII NspI Sau96I
ScrFI SfaNI Tsp509I TspRI