Restriction map for
pFN21AE6026
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 205 GTAGAC GT^[AC][GT]AC
AcsI 31 GAATTC [GA]^AATT[CT]
AfaI 774 GTAC GT^AC
AluI 625 AGCT AG^CT
AlwNI 475 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 31 GAATTC [GA]^AATT[CT]
Asp700I 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 149 GGACC G^G[AT]CC
BamHI 680 GGATCC G^GATCC
BsaJI 186 CCTTGG C^C[ACGT][ACGT]GG
BsgI 244 CTGCAC GTGCAG(16/14)
BsiYI 78 CCTGTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 78 CCTGTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 6 GTCCC GGGAC(10/14)
BspLU11I 446 ACATGT A^CATGT
BsrDI 592 GCAATG GCAATG(2/0)
BstAPI 122 GCAACCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 190 GGTCACC G^GT[ACGT]ACC
BstPI 190 GGTCACC G^GT[ACGT]ACC
CfoI 536 GCGC GCG^C
Cfr13I 149 GGACC G^G[ACGT]CC
DraIII 294 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 571 GACACCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 190 GGTCACC G^GT[ACGT]ACC
EcoRI 31 GAATTC G^AATTC
EcoT14I 186 CCTTGG C^C[AT][AT]GG
HhaI 536 GCGC GCG^C
HinP1I 536 GCGC G^CGC
HinfI 808 GAGTC G^A[ACGT]TC
HphI 192 TCACC GGTGA(8/7)
MseI 461 TTAA T^TAA
MslI 18 CACCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 808 GAGTC GAGTC(4/5)
PshAI 735 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 774 GTAC GT^AC
Sau96I 149 GGACC G^G[ACGT]CC
SmlI 621 CTCAAG C^T[CT][GA]AG
SnaBI 775 TACGTA TAC^GTA
StuI 781 AGGCCT AGG^CCT
StyI 186 CCTTGG C^C[AT][AT]GG
Tru9I 461 TTAA T^TAA
XmnI 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AflIII 446 ACATGT A^C[GA][CT]GT
718 ACGTGT
BanII 466 GGGCTC G[GA]GC[CT]^C
843 GAGCCC
BbsI 267 GAAGAC GAAGAC(2/6)
302 GTCTTC
BclI 143 TGATCA T^GATCA
323 TGATCA
BfaI 67 CTAG C^TAG
319 CTAG
BmyI 466 GGGCTC G[AGT]GC[ACT]^C
843 GAGCCC
BpuAI 267 GAAGAC GAAGAC(2/6)
302 GTCTTC
BsaAI 717 TACGTG [CT]AC^GT[GA]
775 TACGTA
BseRI 545 CTCCTC GAGGAG(10/8)
805 GAGGAG
Bsp1286I 466 GGGCTC G[AGT]GC[ACT]^C
843 GAGCCC
BsrI 114 ACTGG ACTGG(1/-1)
61 CCAGT
BssKI 93 CCTGG ^CC[ACGT]GG
401 CCAGG
BstF5I 286 GGATG GGATG(2/0)
404 GGATG
BstNI 93 CCTGG CC^[AT]GG
401 CCAGG
BstYI 74 AGATCC [GA]^GATC[CT]
680 GGATCC
EcoRII 93 CCTGG ^CC[AT]GG
401 CCAGG
FbaI 143 TGATCA T^GATCA
323 TGATCA
FokI 286 GGATG GGATG(9/13)
404 GGATG
HaeIII 561 GGCC GG^CC
782 GGCC
MaeI 67 CTAG C^TAG
319 CTAG
MaeII 718 ACGT A^CGT
776 ACGT
MflI 74 AGATCC [GA]^GATC[CT]
680 GGATCC
MvaI 93 CCTGG CC^[AT]GG
401 CCAGG
MwoI 122 GCAACCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
346 GCTTCAATGGC
NspI 446 ACATGT [GA]CATG^[CT]
770 GCATGT
ScrFI 93 CCTGG CC^[ACGT]GG
401 CCAGG
SfaNI 287 GATGC GCATC(5/9)
422 GCATC
SfcI 800 CTGTAG C^T[GA][CT]AG
867 CTATAG
XhoII 74 AGATCC [GA]^GATC[CT]
680 GGATCC
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AlwI BbvI Cac8I DdeI DpnII
EarI Fnu4HI ItaI Ksp632I MaeIII
MboI MboII MnlI NdeII NlaIII
NlaIV Sau3AI TseI Tsp45I Tsp509I
TspRI