Restriction map for
pFN21AE6025
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 205 GTAGAC GT^[AC][GT]AC
AcsI 31 GAATTC [GA]^AATT[CT]
AfaI 774 GTAC GT^AC
AluI 625 AGCT AG^CT
AlwNI 475 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 31 GAATTC [GA]^AATT[CT]
Asp700I 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 149 GGACC G^G[AT]CC
BalI 189 TGGCCA TGG^CCA
BamHI 680 GGATCC G^GATCC
BfaI 67 CTAG C^TAG
BsgI 244 CTGCAC GTGCAG(16/14)
BsiYI 78 CCTGTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 78 CCTGTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 45 GGGAC GGGAC(10/14)
BspLU11I 446 ACATGT A^CATGT
BspMI 161 ACCTGC ACCTGC(4/8)
BsrDI 592 GCAATG GCAATG(2/0)
BstAPI 122 GCAACCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 536 GCGC GCG^C
Cfr13I 149 GGACC G^G[ACGT]CC
DraIII 294 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 571 GACACCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 189 TGGCCA [CT]^GGCC[GA]
EcoRI 31 GAATTC G^AATTC
EcoT14I 186 CCTTGG C^C[AT][AT]GG
HhaI 536 GCGC GCG^C
HinP1I 536 GCGC G^CGC
HinfI 808 GAGTC G^A[ACGT]TC
HphI 321 GGTGA GGTGA(8/7)
MaeI 67 CTAG C^TAG
MluNI 189 TGGCCA TGG^CCA
MscI 189 TGGCCA TGG^CCA
MseI 614 TTAA T^TAA
MslI 18 CACCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 808 GAGTC GAGTC(4/5)
PshAI 735 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 821 CTGCAG CTGCA^G
RsaI 774 GTAC GT^AC
Sau96I 149 GGACC G^G[ACGT]CC
SfaNI 287 GATGC GCATC(5/9)
SmlI 621 CTCAAG C^T[CT][GA]AG
SnaBI 775 TACGTA TAC^GTA
StuI 781 AGGCCT AGG^CCT
StyI 186 CCTTGG C^C[AT][AT]GG
Tru9I 614 TTAA T^TAA
XmnI 31 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 446 ACATGT A^C[GA][CT]GT
718 ACGTGT
BbsI 267 GAAGAC GAAGAC(2/6)
302 GTCTTC
BclI 143 TGATCA T^GATCA
323 TGATCA
BpuAI 267 GAAGAC GAAGAC(2/6)
302 GTCTTC
BsaAI 717 TACGTG [CT]AC^GT[GA]
775 TACGTA
BsaJI 186 CCTTGG C^C[ACGT][ACGT]GG
317 CCCTGG
BseRI 545 CTCCTC GAGGAG(10/8)
805 GAGGAG
BsrI 114 ACTGG ACTGG(1/-1)
61 CCAGT
BssKI 318 CCTGG ^CC[ACGT]GG
401 CCAGG
BstNI 318 CCTGG CC^[AT]GG
401 CCAGG
BstYI 74 AGATCC [GA]^GATC[CT]
680 GGATCC
EcoRII 318 CCTGG ^CC[AT]GG
401 CCAGG
FbaI 143 TGATCA T^GATCA
323 TGATCA
MaeII 718 ACGT A^CGT
776 ACGT
MflI 74 AGATCC [GA]^GATC[CT]
680 GGATCC
MvaI 318 CCTGG CC^[AT]GG
401 CCAGG
MwoI 122 GCAACCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
346 GCTTCAATGGC
NspI 446 ACATGT [GA]CATG^[CT]
770 GCATGT
ScrFI 318 CCTGG CC^[ACGT]GG
401 CCAGG
XhoII 74 AGATCC [GA]^GATC[CT]
680 GGATCC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AlwI BbvI BstF5I Cac8I DdeI
DpnII EarI Fnu4HI FokI HaeIII
ItaI Ksp632I MaeIII MboI MboII
MnlI NdeII NlaIII NlaIV Sau3AI
SfcI TseI Tsp45I Tsp509I TspRI