Restriction map for
pFN21AE6024
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbsI
BcgI BciVI BcnI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MamI MluI MroI MspI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 31 GAATTT [GA]^AATT[CT]
AfaI 180 GTAC GT^AC
AlwNI 605 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 31 GAATTT [GA]^AATT[CT]
BamHI 649 GGATCC G^GATCC
BanII 930 GGGCTC G[GA]GC[CT]^C
BbvCI 694 GCTGAGG CCTCAGC(-5/-2)
BclI 257 TGATCA T^GATCA
BsmAI 745 GTCTC GTCTC(1/5)
BsmFI 250 GGGAC GGGAC(10/14)
BsmI 712 GCATTC GAATGC(1/-1)
BsrDI 125 GCAATG GCAATG(2/0)
BssSI 40 CTCGTG CACGAG(-5/-1)
BstYI 649 GGATCC [GA]^GATC[CT]
Cac8I 432 GCTGGC GC[ACGT]^[ACGT]GC
DsaI 353 CCATGG C^C[GA][CT]GG
FbaI 257 TGATCA T^GATCA
MfeI 20 CAATTG C^AATTG
MflI 649 GGATCC [GA]^GATC[CT]
MseI 118 TTAA T^TAA
MunI 20 CAATTG C^AATTG
NcoI 353 CCATGG C^CATGG
NdeI 359 CATATG CA^TATG
PflMI 732 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 18 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 180 GTAC GT^AC
SmlI 876 CTCAAG C^T[CT][GA]AG
Tru9I 118 TTAA T^TAA
Van91I 732 CCACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 649 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
525 GAACCACTTC
AvaII 251 GGACC G^G[AT]CC
923 GGACC
BglI 349 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
427 GCCCAGCTGGC
BpmI 208 CTGGAG CTGGAG(16/14)
592 CTGGAG
BsaAI 370 TACGTG [CT]AC^GT[GA]
774 CACGTA
BsgI 161 CTGCAC GTGCAG(16/14)
899 CTGCAC
BstXI 101 CCATTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
239 CCAAGATGCTGG
DraII 250 GGGACCC [GA]G^G[ACGT]CC[CT]
922 AGGACCC
EcoO109I 250 GGGACCC [GA]G^G[ACGT]CC[CT]
922 AGGACCC
EcoT14I 285 CCTTGG C^C[AT][AT]GG
353 CCATGG
HinfI 157 GACTC G^A[ACGT]TC
846 GACTC
HphI 522 GGTGA GGTGA(8/7)
843 GGTGA
MaeII 371 ACGT A^CGT
775 ACGT
PleI 157 GACTC GAGTC(4/5)
846 GACTC
PpuMI 250 GGGACCC [GA]G^G[AT]CC[CT]
922 AGGACCC
StyI 285 CCTTGG C^C[AT][AT]GG
353 CCATGG
Tsp509I 21 AATT ^AATT
32 AATT
XmnI 31 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
525 GAACCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI BalI BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EaeI EarI EcoRII
Fnu4HI FokI HaeIII ItaI Ksp632I
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI