Restriction map for
pFN21AE6013
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbvCI BcgI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I FauI FseI FspI HaeII
HapII HindIII HpaI HpaII KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmeI PpuMI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
StyI SwaI XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 71 enzymes) #
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AccI 618 GTCGAC GT^[AC][GT]AC
AciI 116 CCGC CCGC(-3/-1)
AcsI 434 AAATTT [GA]^AATT[CT]
AcyI 800 GACGCC G[GA]^CG[CT]C
AflIII 77 ACATGT A^C[GA][CT]GT
Alw44I 231 GTGCAC G^TGCAC
AocI 241 CCTCAGG CC^T[ACGT]AGG
ApaLI 231 GTGCAC G^TGCAC
ApoI 434 AAATTT [GA]^AATT[CT]
AspHI 231 GTGCAC G[AT]GC[AT]^C
AspI 214 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 69 GGACC G^G[AT]CC
BbrPI 228 CACGTG CAC^GTG
BbvI 164 GCAGC GCAGC(8/12)
BciVI 818 GTATCC GTATCC(6/5)
BclI 425 TGATCA T^GATCA
BmyI 231 GTGCAC G[AGT]GC[ACT]^C
BsaAI 228 CACGTG [CT]AC^GT[GA]
BsaBI 84 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 800 GACGCC G[GA]^CG[CT]C
BsiHKAI 231 GTGCAC G[AT]GC[AT]^C
BsmAI 448 GTCTC GTCTC(1/5)
BsmFI 275 GGGAC GGGAC(10/14)
BsmI 845 GCATTC GAATGC(1/-1)
Bsp1286I 231 GTGCAC G[AGT]GC[ACT]^C
BspLU11I 77 ACATGT A^CATGT
BstBI 581 TTCGAA TT^CGAA
BstXI 789 CCAGAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 344 GGATCT [GA]^GATC[CT]
Bsu36I 241 CCTCAGG CC^T[ACGT]AGG
CfoI 162 GCGC GCG^C
Cfr13I 69 GGACC G^G[ACGT]CC
DraI 492 TTTAAA TTT^AAA
Eco81I 241 CCTCAGG CC^T[ACGT]AGG
EcoT22I 566 ATGCAT ATGCA^T
FbaI 425 TGATCA T^GATCA
Fnu4HI 164 GCAGC GC^[ACGT]GC
HgaI 800 GACGC GACGC(5/10)
HhaI 162 GCGC GCG^C
Hin1I 800 GACGCC G[GA]^CG[CT]C
HinP1I 162 GCGC G^CGC
HincII 618 GTCGAC GT[CT]^[GA]AC
HindII 618 GTCGAC GT[CT]^[GA]AC
ItaI 164 GCAGC GC^[ACGT]GC
MaeII 229 ACGT A^CGT
MamI 84 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 344 GGATCT [GA]^GATC[CT]
MseI 493 TTAA T^TAA
MslI 609 CATTCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 381 CATATG CA^TATG
NsiI 566 ATGCAT ATGCA^T
NspV 581 TTCGAA TT^CGAA
PflFI 214 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 42 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 337 GAGTC GAGTC(4/5)
PmaCI 228 CACGTG CAC^GTG
PmlI 228 CACGTG CAC^GTG
PshAI 621 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 618 GTCGAC G^TCGAC
SapI 306 GCTCTTC GCTCTTC(1/4)
Sau96I 69 GGACC G^G[ACGT]CC
SfcI 64 CTACAG C^T[GA][CT]AG
SfuI 581 TTCGAA TT^CGAA
SphI 197 GCATGC GCATG^C
SspI 520 AATATT AAT^ATT
StuI 245 AGGCCT AGG^CCT
Tru9I 493 TTAA T^TAA
TseI 164 GCAGC G^C[AT]GC
Tth111I 214 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 42 CCACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 344 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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BbsI 37 GTCTTC GAAGAC(2/6)
679 GTCTTC
BfaI 26 CTAG C^TAG
556 CTAG
BpmI 695 CTCCAG CTGGAG(16/14)
206 CTGGAG
BpuAI 37 GTCTTC GAAGAC(2/6)
679 GTCTTC
BsaJI 57 CCCTGG C^C[ACGT][ACGT]GG
284 CCCTGG
EarI 187 CTCTTC CTCTTC(1/4)
307 CTCTTC
HphI 354 GGTGA GGTGA(8/7)
113 TCACC
Ksp632I 187 CTCTTC CTCTTC(1/4)
307 CTCTTC
MaeI 26 CTAG C^TAG
556 CTAG
MaeIII 112 GTCAC ^GT[ACGT]AC
442 GTGAC
MwoI 53 GCATCCCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
559 GCCAAATATGC
TaqI 582 TCGA T^CGA
619 TCGA
TfiI 666 GATTC G^A[AT]TC
867 GAATC
Tsp45I 112 GTCAC ^GT[GC]AC
442 GTGAC
TspRI 18 CAGTG CA[GC]TG[ACGT][ACGT]^
392 CACTG
TthHB8I 582 TCGA T^CGA
619 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI AlwI BsiYI BslI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII FokI HaeIII HinfI
MboI MboII MnlI MvaI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
ScrFI SfaNI Tsp509I