Restriction map for
pFN21AE5992
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cac8I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 211 GTAGAC GT^[AC][GT]AC
AcsI 4 GAATTT [GA]^AATT[CT]
AflIII 23 ACACGT A^C[GA][CT]GT
ApoI 4 GAATTT [GA]^AATT[CT]
BbsI 711 GAAGAC GAAGAC(2/6)
BclI 149 TGATCA T^GATCA
BfaI 416 CTAG C^TAG
BglI 352 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 296 GTGCCC G[AGT]GC[ACT]^C
BpuAI 711 GAAGAC GAAGAC(2/6)
BsgI 570 CTGCAC GTGCAG(16/14)
BsiYI 284 CCTATTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 284 CCTATTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 763 GGGAC GGGAC(10/14)
Bsp1286I 296 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 398 TGTACA T^GTACA
BsrBI 690 CCGCTC CCGCTC(-3/-3)
BsrGI 398 TGTACA T^GTACA
BsrI 853 CCAGT ACTGG(1/-1)
BstXI 242 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 686 AGATCC [GA]^GATC[CT]
CfoI 728 GCGC GCG^C
Cfr13I 788 GGCCC G^G[ACGT]CC
DsaI 356 CCATGG C^C[GA][CT]GG
EaeI 350 TGGCCG [CT]^GGCC[GA]
FbaI 149 TGATCA T^GATCA
HhaI 728 GCGC GCG^C
HinP1I 728 GCGC G^CGC
MaeI 416 CTAG C^TAG
MaeII 25 ACGT A^CGT
MflI 686 AGATCC [GA]^GATC[CT]
MspA1I 905 CAGCTG C[AC]G^C[GT]G
MwoI 352 GCCGCCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 356 CCATGG C^CATGG
NlaIV 596 GGCTCC GG[ACGT]^[ACGT]CC
NspI 724 ACATGC [GA]CATG^[CT]
PleI 765 GACTC GAGTC(4/5)
PvuII 905 CAGCTG CAG^CTG
Sau96I 788 GGCCC G^G[ACGT]CC
SfiI 351 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 398 TGTACA T^GTACA
SspI 506 AATATT AAT^ATT
XhoII 686 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 320 GTAC GT^AC
399 GTAC
AlwI 160 GATCC GGATC(4/5)
687 GATCC
AseI 526 ATTAAT AT^TAAT
586 ATTAAT
AsnI 526 ATTAAT AT^TAAT
586 ATTAAT
BbvI 614 GCAGC GCAGC(8/12)
904 GCAGC
BseRI 598 CTCCTC GAGGAG(10/8)
769 CTCCTC
BssKI 348 CCTGG ^CC[ACGT]GG
474 CCTGG
BstNI 348 CCTGG CC^[AT]GG
474 CCTGG
EcoRII 348 CCTGG ^CC[AT]GG
474 CCTGG
HphI 165 TCACC GGTGA(8/7)
735 TCACC
MvaI 348 CCTGG CC^[AT]GG
474 CCTGG
PshBI 526 ATTAAT AT^TAAT
586 ATTAAT
PstI 73 CTGCAG CTGCA^G
301 CTGCAG
RsaI 320 GTAC GT^AC
399 GTAC
ScrFI 348 CCTGG CC^[ACGT]GG
474 CCTGG
SfcI 73 CTGCAG C^T[GA][CT]AG
301 CTGCAG
TseI 614 GCAGC G^C[AT]GC
904 GCAGC
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI BsaJI BstF5I DdeI
DpnII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MboI MboII
MnlI MseI MslI NdeII NlaIII
Sau3AI SfaNI StyI TfiI Tru9I
Tsp45I Tsp509I TspRI