##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflIII      AgeI        AhdI        
AlwNI       Aor51HI     ApaI        ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BanI        BbeI        BbrPI       BcgI        BclI        
BcnI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeII       MaeIII      
MamI        MfeI        MluI        MroI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Tsp45I      Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          225  GTCTAC               GT^[AC][GT]AC
AciI          369  CCGC                 CCGC(-3/-1)
AflII         495  CTTAAG               C^TTAAG
Alw44I         73  GTGCAC               G^TGCAC
AocI          453  CCTGAGG              CC^T[ACGT]AGG
ApaLI          73  GTGCAC               G^TGCAC
BamHI         662  GGATCC               G^GATCC
BanII         175  GAGCCC               G[GA]GC[CT]^C
BbsI          676  GTCTTC               GAAGAC(2/6)
BbvCI         912  GCTGAGG              CCTCAGC(-5/-2)
BbvI          133  GCTGC                GCAGC(8/12)
BciVI          53  GTATCC               GTATCC(6/5)
BfaI           64  CTAG                 C^TAG
BfrI          495  CTTAAG               C^TTAAG
BglI          559  GCCCGTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         676  GTCTTC               GAAGAC(2/6)
Bsp1407I      464  TGTACA               T^GTACA
BsrGI         464  TGTACA               T^GTACA
BstAPI        439  GCAGGCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         242  CCAAAATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         662  GGATCC               [GA]^GATC[CT]
Bsu36I        453  CCTGAGG              CC^T[ACGT]AGG
DsaI          356  CCATGG               C^C[GA][CT]GG
Eco81I        453  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       356  CCATGG               C^C[AT][AT]GG
Fnu4HI        133  GCTGC                GC^[ACGT]GC
HincII        586  GTCAAC               GT[CT]^[GA]AC
HindII        586  GTCAAC               GT[CT]^[GA]AC
HphI          413  TCACC                GGTGA(8/7)
ItaI          133  GCTGC                GC^[ACGT]GC
MaeI           64  CTAG                 C^TAG
MflI          662  GGATCC               [GA]^GATC[CT]
MseI          496  TTAA                 T^TAA
NcoI          356  CCATGG               C^CATGG
NlaIV         662  GGATCC               GG[ACGT]^[ACGT]CC
SfcI            5  CTATAG               C^T[GA][CT]AG
SmlI          495  CTTAAG               C^T[CT][GA]AG
SspBI         464  TGTACA               T^GTACA
StyI          356  CCATGG               C^C[AT][AT]GG
TaqI          702  TCGA                 T^CGA
Tru9I         496  TTAA                 T^TAA
TseI          133  GCTGC                G^C[AT]GC
TthHB8I       702  TCGA                 T^CGA
XhoII         662  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BseRI         490  CTCCTC               GAGGAG(10/8)
              456  GAGGAG              
BsmAI         904  GAGAC                GTCTC(1/5)
              167  GTCTC               
BsrI          480  ACTGG                ACTGG(1/-1)
               21  CCAGT               
MslI          327  CACTGTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              733  CACATTGGTG          
MwoI          439  GCAGGCATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              559  GCCCGTCTGGC         
PleI          704  GACTC                GAGTC(4/5)
              165  GAGTC               
TfiI           89  GATTC                G^A[AT]TC
              205  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AfaI        AluI        AlwI        AspHI       BalI        
BmyI        BsaJI       BsiHKAI     BsiYI       BslI        
Bsp1286I    BsrDI       BssKI       BstF5I      BstNI       
Cac8I       DdeI        DpnII       EaeI        EcoRII      
FokI        HaeIII      HinfI       MboI        MboII       
MluNI       MnlI        MscI        MvaI        NdeII       
NlaIII      RsaI        Sau3AI      ScrFI       SfaNI       
TspRI