Restriction map for
pFN21AE5979
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspI AvaI AviII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BbvI BcgI BcnI BfrI
BglI BglII BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TseI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 644 ACGTGT A^C[GA][CT]GT
AlwI 288 GATCC GGATC(4/5)
AlwNI 408 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 511 ATTAAT AT^TAAT
AsnI 511 ATTAAT AT^TAAT
AvaII 638 GGTCC G^G[AT]CC
AvrII 249 CCTAGG C^CTAGG
BanII 615 GAGCTC G[GA]GC[CT]^C
BciVI 671 GTATCC GTATCC(6/5)
BclI 147 TGATCA T^GATCA
BfaI 250 CTAG C^TAG
BlnI 249 CCTAGG C^CTAGG
BsaAI 643 TACGTG [CT]AC^GT[GA]
BsaI 771 GAGACC GGTCTC(1/5)
BsgI 382 CTGCAC GTGCAG(16/14)
BsmFI 316 GGGAC GGGAC(10/14)
BsmI 625 GCATTC GAATGC(1/-1)
Bsp1407I 647 TGTACA T^GTACA
BsrGI 647 TGTACA T^GTACA
BstAPI 407 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 62 TTCGAA TT^CGAA
BstEII 831 GGTGACC G^GT[ACGT]ACC
BstPI 831 GGTGACC G^GT[ACGT]ACC
DsaI 744 CCACGG C^C[GA][CT]GG
EaeI 809 TGGCCG [CT]^GGCC[GA]
EcoO65I 831 GGTGACC G^GT[ACGT]ACC
EcoRV 202 GATATC GAT^ATC
EcoT14I 249 CCTAGG C^C[AT][AT]GG
FbaI 147 TGATCA T^GATCA
Fnu4HI 811 GCCGC GC^[ACGT]GC
HindIII 698 AAGCTT A^AGCTT
ItaI 811 GCCGC GC^[ACGT]GC
MaeI 250 CTAG C^TAG
MaeII 644 ACGT A^CGT
MspA1I 812 CCGCTG C[AC]G^C[GT]G
NspV 62 TTCGAA TT^CGAA
PpuMI 637 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 511 ATTAAT AT^TAAT
SacI 615 GAGCTC GAGCT^C
SfaNI 434 GATGC GCATC(5/9)
SfuI 62 TTCGAA TT^CGAA
SmlI 864 CTTGAG C^T[CT][GA]AG
SspBI 647 TGTACA T^GTACA
StyI 249 CCTAGG C^C[AT][AT]GG
TaqI 63 TCGA T^CGA
TthHB8I 63 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 12 GAGCAC G[AT]GC[AT]^C
615 GAGCTC
BmyI 12 GAGCAC G[AGT]GC[ACT]^C
615 GAGCTC
BpmI 9 CTGGAG CTGGAG(16/14)
55 CTGGAG
BseRI 592 CTCCTC GAGGAG(10/8)
786 CTCCTC
BsiHKAI 12 GAGCAC G[AT]GC[AT]^C
615 GAGCTC
Bsp1286I 12 GAGCAC G[AGT]GC[ACT]^C
615 GAGCTC
BspHI 398 TCATGA T^CATGA
914 TCATGA
BsrDI 531 CATTGC GCAATG(2/0)
738 CATTGC
DdeI 181 CTCAG C^T[ACGT]AG
613 CTGAG
DpnII 148 GATC ^GATC
288 GATC
DraII 409 AGGGCCT [GA]G^G[ACGT]CC[CT]
637 GGGTCCT
EcoO109I 409 AGGGCCT [GA]G^G[ACGT]CC[CT]
637 GGGTCCT
HinfI 60 GATTC G^A[ACGT]TC
911 GAATC
MboI 148 GATC ^GATC
288 GATC
MseI 103 TTAA T^TAA
512 TTAA
MwoI 40 GCCTTCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
407 GCAGGGCCTGC
NdeII 148 GATC ^GATC
288 GATC
NlaIV 445 GGAGCC GG[ACGT]^[ACGT]CC
637 GGGTCC
RcaI 398 TCATGA T^CATGA
914 TCATGA
Sau3AI 148 GATC ^GATC
288 GATC
SfcI 498 CTGTAG C^T[GA][CT]AG
858 CTACAG
TfiI 60 GATTC G^A[AT]TC
911 GAATC
Tru9I 103 TTAA T^TAA
512 TTAA
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AfaI AluI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I EarI EcoRII
FokI HaeIII HphI Ksp632I MaeIII
MboII MnlI MslI MvaI NlaIII
RsaI Sau96I ScrFI Tsp45I Tsp509I
TspRI