Restriction map for
pFN21AE5961
##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AvrII
BalI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI HaeII HgaI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 829 GTCTAC GT^[AC][GT]AC
AfaI 177 GTAC GT^AC
AflIII 718 ACGTGT A^C[GA][CT]GT
AlwNI 814 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 875 TGCGCA TGC^GCA
BbrPI 717 CACGTG CAC^GTG
BbvCI 686 CCTCAGC CCTCAGC(-5/-2)
BcnI 158 CCCGG CC^[GC]GG
BglII 299 AGATCT A^GATCT
BsaAI 717 CACGTG [CT]AC^GT[GA]
BseRI 903 GAGGAG GAGGAG(10/8)
BsmAI 290 GTCTC GTCTC(1/5)
BspMI 125 ACCTGC ACCTGC(4/8)
BstAPI 424 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 876 GCGC GCG^C
DraII 815 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 416 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 356 CCTACGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 815 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 318 CCTTGG C^C[AT][AT]GG
FspI 875 TGCGCA TGC^GCA
HhaI 876 GCGC GCG^C
HinP1I 876 GCGC G^CGC
HinfI 154 GACTC G^A[ACGT]TC
MaeIII 536 GTGAC ^GT[ACGT]AC
NciI 158 CCCGG CC^[GC]GG
PleI 154 GACTC GAGTC(4/5)
PmaCI 717 CACGTG CAC^GTG
PmlI 717 CACGTG CAC^GTG
PstI 295 CTGCAG CTGCA^G
RsaI 177 GTAC GT^AC
SapI 789 GAAGAGC GCTCTTC(1/4)
StyI 318 CCTTGG C^C[AT][AT]GG
Tsp45I 536 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwI 618 GATCC GGATC(4/5)
639 GATCC
AspHI 673 GTGCTC G[AT]GC[AT]^C
838 GTGCTC
BfaI 589 CTAG C^TAG
622 CTAG
BmyI 673 GTGCTC G[AGT]GC[ACT]^C
838 GTGCTC
BpmI 58 CTCCAG CTGGAG(16/14)
15 CTGGAG
BsiHKAI 673 GTGCTC G[AT]GC[AT]^C
838 GTGCTC
BsmI 191 GCATTC GAATGC(1/-1)
518 GCATTC
Bsp1286I 673 GTGCTC G[AGT]GC[ACT]^C
838 GTGCTC
BspHI 107 TCATGA T^CATGA
404 TCATGA
BsrFI 380 GCCGGC [GA]^CCGG[CT]
694 GCCGGC
BstF5I 231 CATCC GGATG(2/0)
762 CATCC
BstYI 299 AGATCT [GA]^GATC[CT]
617 AGATCC
Cfr10I 380 GCCGGC [GA]^CCGG[CT]
694 GCCGGC
EarI 79 CTCTTC CTCTTC(1/4)
789 GAAGAG
EcoRI 31 GAATTC G^AATTC
680 GAATTC
FokI 231 CATCC GGATG(9/13)
762 CATCC
Ksp632I 79 CTCTTC CTCTTC(1/4)
789 GAAGAG
MaeI 589 CTAG C^TAG
622 CTAG
MflI 299 AGATCT [GA]^GATC[CT]
617 AGATCC
MwoI 424 GCAGAGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
509 GCCCCAATCGC
NaeI 380 GCCGGC GCC^GGC
694 GCCGGC
NgoMIV 380 GCCGGC G^CCGGC
694 GCCGGC
PflMI 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
813 CCAGGCCCTGG
RcaI 107 TCATGA T^CATGA
404 TCATGA
SfaNI 240 GATGC GCATC(5/9)
761 GCATC
SfcI 295 CTGCAG C^T[GA][CT]AG
867 CTACAG
Van91I 499 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
813 CCAGGCCCTGG
XhoII 299 AGATCT [GA]^GATC[CT]
617 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsaJI
BsgI BsiYI BslI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII HapII HpaII
ItaI MaeII MboI MboII MnlI
MslI MspA1I MspI MvaI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI TseI Tsp509I TspRI