Restriction map for
pFN21AE5953
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AgeI AhdI AlwI
AlwNI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BglI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspEI BsrBI BsrDI BsrGI
BsrI BssHII Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I CelII CpoI
DraI DraIII EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI Tsp45I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 274 CGCG CG^CG
AflII 924 CTTAAG C^TTAAG
AflIII 723 ACATGT A^C[GA][CT]GT
Alw44I 503 GTGCAC G^TGCAC
AocI 417 CCTGAGG CC^T[ACGT]AGG
ApaLI 503 GTGCAC G^TGCAC
AspHI 503 GTGCAC G[AT]GC[AT]^C
AspI 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 36 CCTAGG C^CTAGG
BbsI 574 GTCTTC GAAGAC(2/6)
BcnI 144 CCGGG CC^[GC]GG
BfaI 37 CTAG C^TAG
BfrI 924 CTTAAG C^TTAAG
BglII 293 AGATCT A^GATCT
BlnI 36 CCTAGG C^CTAGG
BpuAI 574 GTCTTC GAAGAC(2/6)
BsaWI 253 ACCGGA [AT]^CCGG[AT]
BsiHKAI 503 GTGCAC G[AT]GC[AT]^C
BspDI 514 ATCGAT AT^CGAT
BspLU11I 723 ACATGT A^CATGT
BspMI 712 ACCTGC ACCTGC(4/8)
BsrFI 152 ACCGGC [GA]^CCGG[CT]
BssSI 508 CACGAG CACGAG(-5/-1)
BstBI 577 TTCGAA TT^CGAA
BstUI 274 CGCG CG^CG
BstYI 293 AGATCT [GA]^GATC[CT]
Bsu36I 417 CCTGAGG CC^T[ACGT]AGG
Cfr10I 152 ACCGGC [GA]^CCGG[CT]
ClaI 514 ATCGAT AT^CGAT
DraII 533 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 565 GACACTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 344 CCATGG C^C[GA][CT]GG
Eco81I 417 CCTGAGG CC^T[ACGT]AGG
EcoO109I 533 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 386 GATATC GAT^ATC
MaeI 37 CTAG C^TAG
MaeII 203 ACGT A^CGT
MflI 293 AGATCT [GA]^GATC[CT]
MvnI 274 CGCG CG^CG
NciI 144 CCGGG CC^[GC]GG
NcoI 344 CCATGG C^CATGG
NspV 577 TTCGAA TT^CGAA
PflFI 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 466 CTGCAG CTGCA^G
SapI 716 GCTCTTC GCTCTTC(1/4)
SbfI 465 CCTGCAGG CCTGCA^GG
SfaNI 207 GATGC GCATC(5/9)
SfuI 577 TTCGAA TT^CGAA
SmlI 924 CTTAAG C^T[CT][GA]AG
Sse8387I 465 CCTGCAGG CCTGCA^GG
StuI 701 AGGCCT AGG^CCT
Tsp509I 118 AATT ^AATT
Tth111I 426 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 293 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 171 GTAC GT^AC
591 GTAC
BsaI 774 GAGACC GGTCTC(1/5)
649 GGTCTC
BsmAI 774 GAGAC GTCTC(1/5)
650 GTCTC
CfoI 275 GCGC GCG^C
678 GCGC
EarI 73 CTCTTC CTCTTC(1/4)
717 CTCTTC
EcoT14I 36 CCTAGG C^C[AT][AT]GG
344 CCATGG
FauI 272 CCCGC CCCGC(4/6)
692 CCCGC
HhaI 275 GCGC GCG^C
678 GCGC
HinP1I 275 GCGC G^CGC
678 GCGC
Ksp632I 73 CTCTTC CTCTTC(1/4)
717 CTCTTC
MspA1I 409 CAGCTG C[AC]G^C[GT]G
452 CAGCTG
MwoI 156 GCTCCACACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
340 GCAGCCATGGC
NspI 674 ACATGC [GA]CATG^[CT]
723 ACATGT
PleI 41 GACTC GAGTC(4/5)
740 GACTC
PvuII 409 CAGCTG CAG^CTG
452 CAGCTG
RsaI 171 GTAC GT^AC
591 GTAC
StyI 36 CCTAGG C^C[AT][AT]GG
344 CCATGG
TaqI 515 TCGA T^CGA
578 TCGA
TfiI 129 GATTC G^A[AT]TC
138 GATTC
TthHB8I 515 TCGA T^CGA
578 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BsaJI BsgI BsiYI BslI
BsmFI Bsp1286I BspHI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MboI
MboII MnlI MseI MslI MspI
MvaI NdeII NlaIII NlaIV RcaI
Sau3AI Sau96I ScrFI SfcI Tru9I
TseI TspRI