Restriction map for
pFN21AE5940
##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MaeII
MfeI MflI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 255 GGATC GGATC(4/5)
BalI 182 TGGCCA TGG^CCA
BanI 121 GGCACC G^G[CT][GA]CC
BanII 163 GAGCCC G[GA]GC[CT]^C
BciVI 454 GTATCC GTATCC(6/5)
BglI 184 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 163 GAGCCC G[AGT]GC[ACT]^C
BpmI 681 CTCCAG CTGGAG(16/14)
Bsp1286I 163 GAGCCC G[AGT]GC[ACT]^C
BspHI 159 TCATGA T^CATGA
BspMI 792 GCAGGT ACCTGC(4/8)
EaeI 182 TGGCCA [CT]^GGCC[GA]
EcoNI 531 CCTTCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 521 ATGCAT ATGCA^T
MluNI 182 TGGCCA TGG^CCA
MscI 182 TGGCCA TGG^CCA
NcoI 344 CCATGG C^CATGG
NsiI 521 ATGCAT ATGCA^T
NspI 519 ACATGC [GA]CATG^[CT]
PvuII 590 CAGCTG CAG^CTG
RcaI 159 TCATGA T^CATGA
StuI 293 AGGCCT AGG^CCT
Tsp509I 927 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AciI 11 GCGG CCGC(-3/-1)
277 GCGG
AvaI 322 CTCGAG C^[CT]CG[GA]G
410 CTCGAG
BbvI 14 GCAGC GCAGC(8/12)
193 GCTGC
BsaBI 148 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
449 GATTTGTATC
BseRI 478 CTCCTC GAGGAG(10/8)
688 GAGGAG
BsmAI 718 GTCTC GTCTC(1/5)
768 GTCTC
BsoBI 322 CTCGAG C^[CT]CG[GA]G
410 CTCGAG
BsrDI 675 CATTGC GCAATG(2/0)
116 GCAATG
BsrI 37 ACTGG ACTGG(1/-1)
68 ACTGG
Cfr13I 213 GGCCC G^G[ACGT]CC
695 GGGCC
DdeI 382 CTTAG C^T[ACGT]AG
670 CTGAG
DpnII 148 GATC ^GATC
256 GATC
DsaI 230 CCACGG C^C[GA][CT]GG
344 CCATGG
EcoT14I 344 CCATGG C^C[AT][AT]GG
698 CCAAGG
MaeIII 500 GTCAC ^GT[ACGT]AC
751 GTCAC
MamI 148 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
449 GATTTGTATC
MboI 148 GATC ^GATC
256 GATC
MseI 140 TTAA T^TAA
548 TTAA
MslI 502 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
535 CACCAGGATG
MspA1I 9 CAGCGG C[AC]G^C[GT]G
590 CAGCTG
MwoI 184 GCCATGCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
228 GCCCACGGTGC
NdeII 148 GATC ^GATC
256 GATC
PaeR7I 322 CTCGAG C^TCGAG
410 CTCGAG
PleI 242 GAGTC GAGTC(4/5)
325 GAGTC
Sau3AI 148 GATC ^GATC
256 GATC
Sau96I 213 GGCCC G^G[ACGT]CC
695 GGGCC
StyI 344 CCATGG C^C[AT][AT]GG
698 CCAAGG
TaqI 323 TCGA T^CGA
411 TCGA
Tru9I 140 TTAA T^TAA
548 TTAA
TseI 14 GCAGC G^C[AT]GC
193 GCTGC
Tsp45I 500 GTCAC ^GT[GC]AC
751 GTCAC
TspRI 67 CACTG CA[GC]TG[ACGT][ACGT]^
755 CAGTG
TthHB8I 323 TCGA T^CGA
411 TCGA
XhoI 322 CTCGAG C^TCGAG
410 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
BsmI BssKI BstF5I BstNI BstXI
Cac8I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MboII MnlI
MvaI NlaIII NlaIV RsaI ScrFI
SmlI TfiI