Restriction map for
pFN21AE5937
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AlwNI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BanII BbeI
BbrPI BcgI BciVI BcnI BfrI
BglI BglII BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BspDI BspEI BspHI BspMI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HapII HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MroI MseI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 203 ACATGT A^C[GA][CT]GT
Aor51HI 57 AGCGCT AGC^GCT
AspHI 100 GTGCTC G[AT]GC[AT]^C
AvaI 618 CTCGGG C^[CT]CG[GA]G
AvrII 315 CCTAGG C^CTAGG
BbsI 82 GTCTTC GAAGAC(2/6)
BbvCI 572 CCTCAGC CCTCAGC(-5/-2)
BclI 137 TGATCA T^GATCA
BfaI 316 CTAG C^TAG
BlnI 315 CCTAGG C^CTAGG
BlpI 292 GCTCAGC GC^T[ACGT]AGC
BpmI 786 CTCCAG CTGGAG(16/14)
Bpu1102I 292 GCTCAGC GC^T[ACGT]AGC
BpuAI 82 GTCTTC GAAGAC(2/6)
BsaAI 97 TACGTG [CT]AC^GT[GA]
BseRI 121 CTCCTC GAGGAG(10/8)
BsiHKAI 100 GTGCTC G[AT]GC[AT]^C
BsmAI 583 GAGAC GTCTC(1/5)
BsoBI 618 CTCGGG C^[CT]CG[GA]G
Bsp1407I 857 TGTACA T^GTACA
BspLU11I 203 ACATGT A^CATGT
BsrBI 382 CCGCTC CCGCTC(-3/-3)
BsrGI 857 TGTACA T^GTACA
BssSI 414 CTCGTG CACGAG(-5/-1)
Cac8I 10 GCCAGC GC[ACGT]^[ACGT]GC
CelII 292 GCTCAGC GC^T[ACGT]AGC
DraIII 324 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 422 CTCTTC CTCTTC(1/4)
Eco47III 57 AGCGCT AGC^GCT
EcoT14I 315 CCTAGG C^C[AT][AT]GG
FauI 381 CCCGC CCCGC(4/6)
FbaI 137 TGATCA T^GATCA
Ksp632I 422 CTCTTC CTCTTC(1/4)
MaeI 316 CTAG C^TAG
MaeII 98 ACGT A^CGT
MspA1I 282 CAGCTG C[AC]G^C[GT]G
NspI 203 ACATGT [GA]CATG^[CT]
PflMI 434 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 80 GAGTC GAGTC(4/5)
PvuII 282 CAGCTG CAG^CTG
SfaNI 535 GATGC GCATC(5/9)
SfcI 339 CTACAG C^T[GA][CT]AG
SspBI 857 TGTACA T^GTACA
StyI 315 CCTAGG C^C[AT][AT]GG
TfiI 148 GATTC G^A[AT]TC
Tsp509I 233 AATT ^AATT
Van91I 434 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcyI 64 GACGCC G[GA]^CG[CT]C
834 GACGCC
AocI 774 CCTGAGG CC^T[ACGT]AGG
864 CCTGAGG
AvaII 505 GGACC G^G[AT]CC
738 GGTCC
BalI 368 TGGCCA TGG^CCA
428 TGGCCA
BamHI 515 GGATCC G^GATCC
677 GGATCC
BanI 225 GGTGCC G^G[CT][GA]CC
322 GGCACC
BbvI 284 GCTGC GCAGC(8/12)
623 GCTGC
BsaHI 64 GACGCC G[GA]^CG[CT]C
834 GACGCC
BsgI 457 CTGCAC GTGCAG(16/14)
563 GTGCAG
BstYI 515 GGATCC [GA]^GATC[CT]
677 GGATCC
Bsu36I 774 CCTGAGG CC^T[ACGT]AGG
864 CCTGAGG
CfoI 58 GCGC GCG^C
411 GCGC
Eco81I 774 CCTGAGG CC^T[ACGT]AGG
864 CCTGAGG
Fnu4HI 284 GCTGC GC^[ACGT]GC
623 GCTGC
HaeII 57 AGCGCT [GA]GCGC^[CT]
410 GGCGCT
HgaI 64 GACGC GACGC(5/10)
834 GACGC
HhaI 58 GCGC GCG^C
411 GCGC
Hin1I 64 GACGCC G[GA]^CG[CT]C
834 GACGCC
HinP1I 58 GCGC G^CGC
411 GCGC
HinfI 80 GAGTC G^A[ACGT]TC
148 GATTC
ItaI 284 GCTGC GC^[ACGT]GC
623 GCTGC
MflI 515 GGATCC [GA]^GATC[CT]
677 GGATCC
MluNI 368 TGGCCA TGG^CCA
428 TGGCCA
MscI 368 TGGCCA TGG^CCA
428 TGGCCA
TseI 284 GCTGC G^C[AT]GC
623 GCTGC
Tsp45I 220 GTCAC ^GT[GC]AC
598 GTGAC
XcmI 545 CCATCCTGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
788 CCAGGAAAGCTGTGG
XhoII 515 GGATCC [GA]^GATC[CT]
677 GGATCC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AfaI AluI AlwI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EaeI EcoRII FokI HaeIII
HphI MaeIII MboI MboII MnlI
MslI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TspRI