Restriction map for
pFN21AE5909
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# NO CUT ENZYMES ( 154 enzymes) #
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AccI AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaI
AvaII AviII AvrII BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HapII HgaI
HhaI HinP1I HindIII HpaI HpaII
KasI KspI MaeI MamI MfeI
MluI MroI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 208 GACGTC GACGT^C
Acc65I 754 GGTACC G^GTACC
AcsI 905 AAATTC [GA]^AATT[CT]
AcyI 208 GACGTC G[GA]^CG[CT]C
AocI 870 CCTGAGG CC^T[ACGT]AGG
ApoI 905 AAATTC [GA]^AATT[CT]
Asp718I 754 GGTACC G^GTACC
BalI 668 TGGCCA TGG^CCA
BamHI 680 GGATCC G^GATCC
BanI 754 GGTACC G^G[CT][GA]CC
BbsI 795 GTCTTC GAAGAC(2/6)
BglI 346 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 68 GGGCAC G[AGT]GC[ACT]^C
BpmI 256 CTGGAG CTGGAG(16/14)
BpuAI 795 GTCTTC GAAGAC(2/6)
BsaHI 208 GACGTC G[GA]^CG[CT]C
BsaI 454 GGTCTC GGTCTC(1/5)
BseRI 545 CTCCTC GAGGAG(10/8)
BsgI 717 CTGCAC GTGCAG(16/14)
BsmFI 232 GTCCC GGGAC(10/14)
BsmI 46 GCATTC GAATGC(1/-1)
Bsp1286I 68 GGGCAC G[AGT]GC[ACT]^C
BspMI 715 ACCTGC ACCTGC(4/8)
BsrI 512 CCAGT ACTGG(1/-1)
BssKI 696 CCAGG ^CC[ACGT]GG
BstEII 24 GGTGACC G^GT[ACGT]ACC
BstNI 696 CCAGG CC^[AT]GG
BstPI 24 GGTGACC G^GT[ACGT]ACC
BstYI 680 GGATCC [GA]^GATC[CT]
Bsu36I 870 CCTGAGG CC^T[ACGT]AGG
DraII 893 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 284 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 629 CCATGG C^C[GA][CT]GG
EaeI 668 TGGCCA [CT]^GGCC[GA]
Eco81I 870 CCTGAGG CC^T[ACGT]AGG
EcoO109I 893 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 24 GGTGACC G^GT[ACGT]ACC
EcoRII 696 CCAGG ^CC[AT]GG
FauI 419 CCCGC CCCGC(4/6)
Hin1I 208 GACGTC G[GA]^CG[CT]C
HincII 558 GTTGAC GT[CT]^[GA]AC
HindII 558 GTTGAC GT[CT]^[GA]AC
KpnI 754 GGTACC GGTAC^C
MflI 680 GGATCC [GA]^GATC[CT]
MluNI 668 TGGCCA TGG^CCA
MscI 668 TGGCCA TGG^CCA
MseI 553 TTAA T^TAA
MvaI 696 CCAGG CC^[AT]GG
NcoI 629 CCATGG C^CATGG
PflMI 237 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScrFI 696 CCAGG CC^[ACGT]GG
SfaNI 240 GATGC GCATC(5/9)
SfcI 864 CTACAG C^T[GA][CT]AG
SfiI 345 GGCCCTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 329 TCGA T^CGA
Tru9I 553 TTAA T^TAA
Tsp509I 906 AATT ^AATT
TthHB8I 329 TCGA T^CGA
Van91I 237 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 680 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 9 enzymes) #
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AfaI 755 GTAC GT^AC
771 GTAC
AlwNI 238 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
382 CAGCCCCTG
BsmAI 455 GTCTC GTCTC(1/5)
481 GTCTC
EarI 79 CTCTTC CTCTTC(1/4)
745 CTCTTC
Ksp632I 79 CTCTTC CTCTTC(1/4)
745 CTCTTC
MaeII 209 ACGT A^CGT
446 ACGT
PleI 189 GAGTC GAGTC(4/5)
426 GAGTC
RsaI 755 GTAC GT^AC
771 GTAC
TfiI 807 GAATC G^A[AT]TC
852 GAATC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BstF5I Cac8I Cfr13I
DdeI DpnII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MnlI MslI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
SmlI StyI TseI Tsp45I TspRI