Restriction map for
pFN21AB9724
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwNI
AocI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI Eam1105I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MaeI MamI MfeI MluI MroI
MseI MunI NcoI NheI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI StyI SwaI TfiI
Tru9I TspRI Tth111I Van91I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 1237 AAATTC [GA]^AATT[CT]
Alw44I 267 GTGCAC G^TGCAC
AlwI 888 GATCC GGATC(4/5)
ApaLI 267 GTGCAC G^TGCAC
ApoI 1237 AAATTC [GA]^AATT[CT]
BalI 875 TGGCCA TGG^CCA
BpmI 78 CTCCAG CTGGAG(16/14)
BsaAI 240 CACGTA [CT]AC^GT[GA]
BsmBI 154 GAGACG CGTCTC(1/5)
BsmFI 204 GTCCC GGGAC(10/14)
BsrI 1170 ACTGG ACTGG(1/-1)
BstAPI 917 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 887 AGATCC [GA]^GATC[CT]
EcoT22I 37 ATGCAT ATGCA^T
HphI 564 TCACC GGTGA(8/7)
KspI 602 CCGCGG CCGC^GG
MaeIII 847 GTGAC ^GT[ACGT]AC
MflI 887 AGATCC [GA]^GATC[CT]
MluNI 875 TGGCCA TGG^CCA
MscI 875 TGGCCA TGG^CCA
NdeI 571 CATATG CA^TATG
NotI 610 GCGGCCGC GC^GGCCGC
NsiI 37 ATGCAT ATGCA^T
NspI 623 ACATGC [GA]CATG^[CT]
SacII 602 CCGCGG CCGC^GG
SapI 1041 GCTCTTC GCTCTTC(1/4)
SbfI 981 CCTGCAGG CCTGCA^GG
Sse8387I 981 CCTGCAGG CCTGCA^GG
StuI 1109 AGGCCT AGG^CCT
Tsp45I 847 GTGAC ^GT[GC]AC
Tsp509I 1238 AATT ^AATT
XcmI 281 CCACGGTGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 887 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 63 GTAC GT^AC
243 GTAC
Aor51HI 815 AGCGCT AGC^GCT
1097 AGCGCT
BbeI 421 GGCGCC GGCGC^C
1009 GGCGCC
BcgI 131 GCAACCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
718 GCAGCGGCATCG
BseRI 357 CTCCTC GAGGAG(10/8)
997 GAGGAG
BsgI 73 CTGCAC GTGCAG(16/14)
1123 GTGCAG
BsmAI 154 GAGAC GTCTC(1/5)
1215 GAGAC
BspMI 711 GCAGGT ACCTGC(4/8)
926 GCAGGT
BsrFI 382 GCCGGC [GA]^CCGG[CT]
409 GCCGGC
Cfr10I 382 GCCGGC [GA]^CCGG[CT]
409 GCCGGC
EarI 136 CTCTTC CTCTTC(1/4)
1042 CTCTTC
Eco47III 815 AGCGCT AGC^GCT
1097 AGCGCT
HinfI 775 GACTC G^A[ACGT]TC
934 GAGTC
KasI 421 GGCGCC G^GCGCC
1009 GGCGCC
Ksp632I 136 CTCTTC CTCTTC(1/4)
1042 CTCTTC
MslI 195 CATCGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
618 CATGAACATG
NaeI 382 GCCGGC GCC^GGC
409 GCCGGC
NarI 421 GGCGCC GG^CGCC
1009 GGCGCC
NgoMIV 382 GCCGGC G^CCGGC
409 GCCGGC
PleI 775 GACTC GAGTC(4/5)
934 GAGTC
RsaI 63 GTAC GT^AC
243 GTAC
SacI 796 GAGCTC GAGCT^C
1039 GAGCTC
SfoI 421 GGCGCC GGC^GCC
1009 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BglI BmyI BsaHI BsaJI
BsiEI BsiHKAI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EagI EclXI Eco52I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PaeR7I PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
TaqI TseI TthHB8I XhoI XmaCI
XmaI