Restriction map for
pFN21AE3488
##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BbeI
BlnI BsgI BsiEI BsiWI BspDI
BspHI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FseI
HaeII HpaI KasI KpnI KspI
MfeI MluI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SpeI Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 319 GTAGAC GT^[AC][GT]AC
AccIII 2582 TCCGGA T^CCGGA
AcyI 4184 GACGCC G[GA]^CG[CT]C
Alw44I 1934 GTGCAC G^TGCAC
ApaLI 1934 GTGCAC G^TGCAC
Asp700I 742 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 3410 CACGTG CAC^GTG
BbvCI 24 GCTGAGG CCTCAGC(-5/-2)
BclI 2345 TGATCA T^GATCA
BglI 1352 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 4184 GACGCC G[GA]^CG[CT]C
BsaI 4708 GAGACC GGTCTC(1/5)
BsaWI 2582 TCCGGA [AT]^CCGG[AT]
BseAI 2582 TCCGGA T^CCGGA
BsmBI 5030 CGTCTC CGTCTC(1/5)
BspEI 2582 TCCGGA T^CCGGA
BspMI 2133 GCAGGT ACCTGC(4/8)
BstAPI 3853 GCATGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3924 TTCGAA TT^CGAA
EcoNI 5277 CCTGTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1673 GAATTC G^AATTC
EcoRV 1315 GATATC GAT^ATC
EcoT22I 2458 ATGCAT ATGCA^T
FbaI 2345 TGATCA T^GATCA
Hin1I 4184 GACGCC G[GA]^CG[CT]C
HincII 3153 GTCAAC GT[CT]^[GA]AC
HindII 3153 GTCAAC GT[CT]^[GA]AC
HindIII 3781 AAGCTT A^AGCTT
MroI 2582 TCCGGA T^CCGGA
NcoI 1747 CCATGG C^CATGG
NdeI 3871 CATATG CA^TATG
NsiI 2458 ATGCAT ATGCA^T
NspV 3924 TTCGAA TT^CGAA
PaeR7I 5033 CTCGAG C^TCGAG
PmaCI 3410 CACGTG CAC^GTG
PmlI 3410 CACGTG CAC^GTG
PshAI 3037 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2275 CTGCAG CTGCA^G
ScaI 1922 AGTACT AGT^ACT
SfuI 3924 TTCGAA TT^CGAA
SmaI 483 CCCGGG CCC^GGG
SphI 5442 GCATGC GCATG^C
SspI 1816 AATATT AAT^ATT
XcmI 1058 CCAAACCAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 5033 CTCGAG C^TCGAG
XmaCI 483 CCCGGG C^CCGGG
XmaI 483 CCCGGG C^CCGGG
XmnI 742 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1721 ACATGT A^C[GA][CT]GT
3374 ACATGT
AspI 3542 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4206 GACGCTGTC
BciVI 2528 GGATAC GTATCC(6/5)
116 GTATCC
BlpI 3223 GCTGAGC GC^T[ACGT]AGC
3467 GCTCAGC
Bpu1102I 3223 GCTGAGC GC^T[ACGT]AGC
3467 GCTCAGC
BsaBI 2080 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5221 GATGGTGATC
Bsp1407I 4912 TGTACA T^GTACA
5253 TGTACA
BspLU11I 1721 ACATGT A^CATGT
3374 ACATGT
BsrBI 140 GAGCGG CCGCTC(-3/-3)
3430 GAGCGG
BsrGI 4912 TGTACA T^GTACA
5253 TGTACA
CelII 3223 GCTGAGC GC^T[ACGT]AGC
3467 GCTCAGC
DraIII 2925 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4343 CACTCCGTG
DrdI 4591 GACAACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4808 GACCTGAGAGTC
MamI 2080 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5221 GATGGTGATC
PflFI 3542 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4206 GACGCTGTC
PflMI 688 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
882 CCAGGAGGTGG
SapI 871 GAAGAGC GCTCTTC(1/4)
3946 GAAGAGC
SnaBI 3077 TACGTA TAC^GTA
4715 TACGTA
SspBI 4912 TGTACA T^GTACA
5253 TGTACA
Tth111I 3542 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4206 GACGCTGTC
Van91I 688 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
882 CCAGGAGGTGG
XbaI 1192 TCTAGA T^CTAGA
4761 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII AviII BalI
BanI BanII BbsI BbvI BcgI
BcnI BfaI BfrI BglII BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII