Restriction map for
pFN21AE5384
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
Acc65I AccII AccIII AciI AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BcgI BciVI BfaI
BfrI BglI BglII BlnI BmyI
BpmI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRII
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MluI MluNI MroI MscI
MvaI MvnI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 776 GACGTC GACGT^C
AccI 94 GTCTAC GT^[AC][GT]AC
AcsI 326 AAATTT [GA]^AATT[CT]
AcyI 776 GACGTC G[GA]^CG[CT]C
AflIII 712 ACATGT A^C[GA][CT]GT
ApoI 326 AAATTT [GA]^AATT[CT]
BbvCI 151 CCTCAGC CCTCAGC(-5/-2)
BcnI 563 CCGGG CC^[GC]GG
BlpI 680 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 680 GCTCAGC GC^T[ACGT]AGC
BsaHI 776 GACGTC G[GA]^CG[CT]C
BsaJI 107 CCATGG C^C[ACGT][ACGT]GG
BseRI 787 GAGGAG GAGGAG(10/8)
BsgI 530 GTGCAG GTGCAG(16/14)
BsmI 674 GAATGC GAATGC(1/-1)
BspLU11I 712 ACATGT A^CATGT
BsrFI 601 GCCGGC [GA]^CCGG[CT]
BssKI 563 CCGGG ^CC[ACGT]GG
BstYI 534 AGATCC [GA]^GATC[CT]
Cac8I 601 GCCGGC GC[ACGT]^[ACGT]GC
CelII 680 GCTCAGC GC^T[ACGT]AGC
CfoI 678 GCGC GCG^C
Cfr10I 601 GCCGGC [GA]^CCGG[CT]
DsaI 107 CCATGG C^C[GA][CT]GG
EarI 70 CTCTTC CTCTTC(1/4)
EcoT14I 107 CCATGG C^C[AT][AT]GG
HhaI 678 GCGC GCG^C
Hin1I 776 GACGTC G[GA]^CG[CT]C
HinP1I 678 GCGC G^CGC
Ksp632I 70 CTCTTC CTCTTC(1/4)
MaeII 777 ACGT A^CGT
MfeI 368 CAATTG C^AATTG
MflI 534 AGATCC [GA]^GATC[CT]
MspA1I 731 CAGCTG C[AC]G^C[GT]G
MunI 368 CAATTG C^AATTG
NaeI 601 GCCGGC GCC^GGC
NciI 563 CCGGG CC^[GC]GG
NcoI 107 CCATGG C^CATGG
NgoMIV 601 GCCGGC G^CCGGC
NspI 712 ACATGT [GA]CATG^[CT]
PshAI 729 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 731 CAGCTG CAG^CTG
ScrFI 563 CCGGG CC^[ACGT]GG
SfcI 861 CTACAG C^T[GA][CT]AG
StuI 701 AGGCCT AGG^CCT
StyI 107 CCATGG C^C[AT][AT]GG
XhoII 534 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AluI 155 AGCT AG^CT
732 AGCT
AlwI 535 GATCC GGATC(4/5)
837 GATCC
AvaII 566 GGACC G^G[AT]CC
719 GGTCC
BbvI 466 GCAGC GCAGC(8/12)
340 GCTGC
BclI 590 TGATCA T^GATCA
905 TGATCA
BsiYI 557 CCTGTGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
710 CCACATGTGGG
BslI 557 CCTGTGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
710 CCACATGTGGG
BsrDI 306 CATTGC GCAATG(2/0)
660 CATTGC
BstF5I 654 CATCC GGATG(2/0)
879 GGATG
Cfr13I 566 GGACC G^G[ACGT]CC
719 GGTCC
DraII 565 GGGACCT [GA]G^G[ACGT]CC[CT]
718 GGGTCCC
EcoO109I 565 GGGACCT [GA]G^G[ACGT]CC[CT]
718 GGGTCCC
FbaI 590 TGATCA T^GATCA
905 TGATCA
Fnu4HI 340 GCTGC GC^[ACGT]GC
466 GCAGC
FokI 654 CATCC GGATG(9/13)
879 GGATG
HapII 563 CCGG C^CGG
602 CCGG
HpaII 563 CCGG C^CGG
602 CCGG
ItaI 340 GCTGC GC^[ACGT]GC
466 GCAGC
MspI 563 CCGG C^CGG
602 CCGG
MwoI 116 GCAATATCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
340 GCTGCGATGGC
PleI 459 GAGTC GAGTC(4/5)
790 GAGTC
PpuMI 565 GGGACCT [GA]G^G[AT]CC[CT]
718 GGGTCCC
Sau96I 566 GGACC G^G[ACGT]CC
719 GGTCC
TaqI 54 TCGA T^CGA
420 TCGA
TfiI 375 GAATC G^A[AT]TC
849 GAATC
TseI 340 GCTGC G^C[AT]GC
466 GCAGC
TspRI 292 CAGTG CA[GC]TG[ACGT][ACGT]^
833 CAGTG
TthHB8I 54 TCGA T^CGA
420 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AfaI BsmFI DdeI DpnII HaeIII
HinfI HphI MaeIII MboI MboII
MnlI MseI MslI NdeII NlaIII
NlaIV RsaI Sau3AI Tru9I Tsp45I
Tsp509I