Restriction map for
pFN21AE5830
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII Acc65I AccI AclI AcsI
AgeI AhdI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BciVI BclI BglII
BlnI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EcoNI EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmeI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccIII 1094 TCCGGA T^CCGGA
AflII 414 CTTAAG C^TTAAG
BbsI 745 GTCTTC GAAGAC(2/6)
BfaI 2344 CTAG C^TAG
BfrI 414 CTTAAG C^TTAAG
BlpI 2814 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2814 GCTGAGC GC^T[ACGT]AGC
BpuAI 745 GTCTTC GAAGAC(2/6)
BsaI 862 GAGACC GGTCTC(1/5)
BseAI 1094 TCCGGA T^CCGGA
BspEI 1094 TCCGGA T^CCGGA
BstAPI 2799 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 323 AGATCC [GA]^GATC[CT]
CelII 2814 GCTGAGC GC^T[ACGT]AGC
DraIII 1647 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 546 GAAGAG CTCTTC(1/4)
FseI 2610 GGCCGGCC GGCCGG^CC
Ksp632I 546 GAAGAG CTCTTC(1/4)
MaeI 2344 CTAG C^TAG
MflI 323 AGATCC [GA]^GATC[CT]
MroI 1094 TCCGGA T^CCGGA
MseI 415 TTAA T^TAA
NlaIII 33 CATG CATG^
PaeR7I 2068 CTCGAG C^TCGAG
SacI 841 GAGCTC GAGCT^C
SapI 546 GAAGAGC GCTCTTC(1/4)
SgrAI 510 CACCGGCG C[GA]^CCGG[CT]G
TfiI 908 GATTC G^A[AT]TC
Tru9I 415 TTAA T^TAA
Tsp509I 2626 AATT ^AATT
XhoI 2068 CTCGAG C^TCGAG
XhoII 323 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
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AflIII 1646 ACACGT A^C[GA][CT]GT
3170 ACACGT
AlwI 324 GATCC GGATC(4/5)
2169 GATCC
AocI 1920 CCTGAGG CC^T[ACGT]AGG
3112 CCTCAGG
Asp700I 546 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2863 GAAACACTTC
BbvCI 1261 GCTGAGG CCTCAGC(-5/-2)
2689 GCTGAGG
BcgI 740 GCAAGGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1715 GCAAGAGCTTCG
BsaWI 134 TCCGGT [AT]^CCGG[AT]
1094 TCCGGA
BsmAI 862 GAGAC GTCTC(1/5)
2773 GAGAC
BstXI 1196 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2660 CCAACCTGCTGG
Bsu36I 1920 CCTGAGG CC^T[ACGT]AGG
3112 CCTCAGG
DpnII 324 GATC ^GATC
2169 GATC
EagI 1238 CGGCCG C^GGCCG
2262 CGGCCG
EclXI 1238 CGGCCG C^GGCCG
2262 CGGCCG
Eco52I 1238 CGGCCG C^GGCCG
2262 CGGCCG
Eco81I 1920 CCTGAGG CC^T[ACGT]AGG
3112 CCTCAGG
MboI 324 GATC ^GATC
2169 GATC
MboII 546 GAAGA GAAGA(8/7)
746 TCTTC
MslI 131 CATTCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2745 CACTGGTGTG
NdeII 324 GATC ^GATC
2169 GATC
PflMI 2329 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2601 CCAGCCCGTGG
PshAI 2520 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2586 GACCACGGTC
Sau3AI 324 GATC ^GATC
2169 GATC
SfaNI 958 GATGC GCATC(5/9)
1209 GCATC
SfiI 367 GGCCAGTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1537 GGCCAGTGCGGCC
SmlI 414 CTTAAG C^T[CT][GA]AG
2068 CTCGAG
TaqI 2069 TCGA T^CGA
2841 TCGA
TthHB8I 2069 TCGA T^CGA
2841 TCGA
Van91I 2329 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2601 CCAGCCCGTGG
XmnI 546 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2863 GAAACACTTC
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# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwNI Aor51HI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbvI BcnI
BglI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI Eco47III EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeII
MaeIII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NgoMIV NlaIV PleI
PmaCI PmlI PstI PvuII RsaI
SacII Sau96I ScrFI SfcI SfoI
SmaI StuI StyI TseI Tsp45I
TspRI XcmI XmaCI XmaI