Restriction map for
pFN21AA0711
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# NO CUT ENZYMES ( 103 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI Alw44I AlwI ApaLI ApoI
AseI AsnI Asp700I Asp718I AviII
AvrII BamHI BbrPI BciVI BclI
BfaI BfrI BlnI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstXI BstZ17I ClaI CpoI
DraI EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MamI MfeI
MluI MroI MseI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SfaNI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 1795 GACGTC GACGT^C
AccI 979 GTCTAC GT^[AC][GT]AC
AflIII 1379 ACGTGT A^C[GA][CT]GT
AhdI 346 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 280 CCTGAGG CC^T[ACGT]AGG
AscI 1036 GGCGCGCC GG^CGCGCC
AspEI 346 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1138 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1144 GTCTTC GAAGAC(2/6)
BbvCI 164 CCTCAGC CCTCAGC(-5/-2)
BcgI 1576 CGATACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1373 AGATCT A^GATCT
BpuAI 1144 GTCTTC GAAGAC(2/6)
BsaAI 1378 TACGTG [CT]AC^GT[GA]
BsmAI 107 GTCTC GTCTC(1/5)
BspHI 773 TCATGA T^CATGA
BstEII 1234 GGTCACC G^GT[ACGT]ACC
BstF5I 1812 CATCC GGATG(2/0)
BstPI 1234 GGTCACC G^GT[ACGT]ACC
BstYI 1373 AGATCT [GA]^GATC[CT]
Bsu36I 280 CCTGAGG CC^T[ACGT]AGG
DraIII 7 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 970 GACGCGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 346 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 280 CCTGAGG CC^T[ACGT]AGG
EcoNI 1191 CCTGCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1234 GGTCACC G^GT[ACGT]ACC
EcoT14I 1790 CCTTGG C^C[AT][AT]GG
FokI 1812 CATCC GGATG(9/13)
HinfI 142 GAGTC G^A[ACGT]TC
HphI 1236 TCACC GGTGA(8/7)
MaeIII 1235 GTCAC ^GT[ACGT]AC
MflI 1373 AGATCT [GA]^GATC[CT]
PflFI 1138 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 142 GAGTC GAGTC(4/5)
RcaI 773 TCATGA T^CATGA
SacI 268 GAGCTC GAGCT^C
SapI 301 GAAGAGC GCTCTTC(1/4)
SgrAI 1040 CGCCGGCG C[GA]^CCGG[CT]G
StyI 1790 CCTTGG C^C[AT][AT]GG
Tsp45I 1235 GTCAC ^GT[GC]AC
Tth111I 1138 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 668 CCACCGACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1373 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AfaI 385 GTAC GT^AC
408 GTAC
AlwNI 35 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
736 CAGCGGCTG
Aor51HI 1277 AGCGCT AGC^GCT
1469 AGCGCT
ApaI 1110 GGGCCC GGGCC^C
1304 GGGCCC
BalI 698 TGGCCA TGG^CCA
1589 TGGCCA
BlpI 690 GCTGAGC GC^T[ACGT]AGC
1266 GCTCAGC
Bpu1102I 690 GCTGAGC GC^T[ACGT]AGC
1266 GCTCAGC
BsgI 526 GTGCAG GTGCAG(16/14)
1452 GTGCAG
BspMI 845 ACCTGC ACCTGC(4/8)
1360 ACCTGC
CelII 690 GCTGAGC GC^T[ACGT]AGC
1266 GCTCAGC
DpnII 1374 GATC ^GATC
1516 GATC
Eco47III 1277 AGCGCT AGC^GCT
1469 AGCGCT
MboI 1374 GATC ^GATC
1516 GATC
MluNI 698 TGGCCA TGG^CCA
1589 TGGCCA
MscI 698 TGGCCA TGG^CCA
1589 TGGCCA
NdeII 1374 GATC ^GATC
1516 GATC
RsaI 385 GTAC GT^AC
408 GTAC
Sau3AI 1374 GATC ^GATC
1516 GATC
SexAI 422 ACCTGGT A^CC[AT]GGT
1139 ACCAGGT
SfiI 1111 GGCCCCGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1636 GGCCCCACGGGCC
SmlI 340 CTTGAG C^T[CT][GA]AG
1411 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI KasI Ksp632I KspI MaeII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NgoMIV NlaIII NlaIV PflMI PpuMI
PstI PvuII SacII Sau96I ScrFI
SfcI SfoI SmaI TaqI TseI
TspRI TthHB8I Van91I XmaCI XmaI