Restriction map for
pFN21AE5707
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CfoI Cfr10I CpoI DraI DraII
DrdI Eam1105I Eco47III EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MluI MluNI MscI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StyI SwaI Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 1418 GTCTAC GT^[AC][GT]AC
AccIII 161 TCCGGA T^CCGGA
AclI 739 AACGTT AA^CGTT
AcyI 36 GGCGTC G[GA]^CG[CT]C
AfaI 392 GTAC GT^AC
AocI 26 CCTCAGG CC^T[ACGT]AGG
AseI 1240 ATTAAT AT^TAAT
AsnI 1240 ATTAAT AT^TAAT
AspHI 55 GAGCTC G[AT]GC[AT]^C
AvaI 1403 CCCGAG C^[CT]CG[GA]G
AvaII 597 GGTCC G^G[AT]CC
BanI 1822 GGTGCC G^G[CT][GA]CC
BanII 55 GAGCTC G[GA]GC[CT]^C
BbsI 1796 GTCTTC GAAGAC(2/6)
BlpI 52 GCTGAGC GC^T[ACGT]AGC
BmyI 55 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 52 GCTGAGC GC^T[ACGT]AGC
BpuAI 1796 GTCTTC GAAGAC(2/6)
BsaHI 36 GGCGTC G[GA]^CG[CT]C
BsaI 76 GAGACC GGTCTC(1/5)
BseAI 161 TCCGGA T^CCGGA
BsiHKAI 55 GAGCTC G[AT]GC[AT]^C
BsmFI 39 GTCCC GGGAC(10/14)
BsoBI 1403 CCCGAG C^[CT]CG[GA]G
Bsp1286I 55 GAGCTC G[AGT]GC[ACT]^C
BspDI 869 ATCGAT AT^CGAT
BspEI 161 TCCGGA T^CCGGA
BspMI 381 ACCTGC ACCTGC(4/8)
Bsu36I 26 CCTCAGG CC^T[ACGT]AGG
CelII 52 GCTGAGC GC^T[ACGT]AGC
ClaI 869 ATCGAT AT^CGAT
DraIII 727 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 47 CCGTGG C^C[GA][CT]GG
Eco81I 26 CCTCAGG CC^T[ACGT]AGG
EcoNI 199 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1058 ATGCAT ATGCA^T
HgaI 37 GCGTC GACGC(5/10)
Hin1I 36 GGCGTC G[GA]^CG[CT]C
MaeII 740 ACGT A^CGT
MfeI 939 CAATTG C^AATTG
MroI 161 TCCGGA T^CCGGA
MunI 939 CAATTG C^AATTG
NotI 7 GCGGCCGC GC^GGCCGC
NsiI 1058 ATGCAT ATGCA^T
PshAI 1014 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1240 ATTAAT AT^TAAT
Psp1406I 739 AACGTT AA^CGTT
PstI 383 CTGCAG CTGCA^G
RsaI 392 GTAC GT^AC
SacI 55 GAGCTC GAGCT^C
SapI 565 GCTCTTC GCTCTTC(1/4)
SspI 1492 AATATT AAT^ATT
StuI 89 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Asp700I 517 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
718 GAATTTTTTC
AspI 768 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1003 GACCATGTC
BcgI 1168 CGATATTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
294 GCAAAAAATTCG
BcnI 42 CCCGG CC^[GC]GG
72 CCCGG
BsaWI 161 TCCGGA [AT]^CCGG[AT]
594 TCCGGT
BsgI 1048 CTGCAC GTGCAG(16/14)
747 GTGCAG
BsiEI 8 CGGCCG CG[GA][CT]^CG
44 CGGCCG
BsmAI 76 GAGAC GTCTC(1/5)
1232 GAGAC
BstNI 1354 CCTGG CC^[AT]GG
1655 CCTGG
BstYI 154 AGATCC [GA]^GATC[CT]
471 AGATCC
Cac8I 853 GCCTGC GC[ACGT]^[ACGT]GC
979 GCTCGC
EaeI 8 CGGCCG [CT]^GGCC[GA]
44 CGGCCG
EagI 8 CGGCCG C^GGCCG
44 CGGCCG
EclXI 8 CGGCCG C^GGCCG
44 CGGCCG
Eco52I 8 CGGCCG C^GGCCG
44 CGGCCG
EcoRII 1354 CCTGG ^CC[AT]GG
1655 CCTGG
MflI 154 AGATCC [GA]^GATC[CT]
471 AGATCC
MvaI 1354 CCTGG CC^[AT]GG
1655 CCTGG
NciI 42 CCCGG CC^[GC]GG
72 CCCGG
NlaIV 449 GGAACC GG[ACGT]^[ACGT]CC
1822 GGTGCC
NspI 540 GCATGT [GA]CATG^[CT]
1060 GCATGT
PflFI 768 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1003 GACCATGTC
PleI 191 GACTC GAGTC(4/5)
502 GACTC
PvuII 289 CAGCTG CAG^CTG
1891 CAGCTG
SfaNI 850 GATGC GCATC(5/9)
1057 GATGC
TfiI 872 GATTC G^A[AT]TC
1573 GAATC
Tth111I 768 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1003 GACCATGTC
XbaI 513 TCTAGA T^CTAGA
775 TCTAGA
XhoII 154 AGATCC [GA]^GATC[CT]
471 AGATCC
XmnI 517 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
718 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BfaI BpmI BsaJI BseRI
BsiYI BslI BsrI BssKI BstF5I
Cfr13I DdeI DpnII EarI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MwoI NdeII
NlaIII Sau3AI Sau96I ScrFI SfcI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I