Restriction map for
pFN21AE5658
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspI AvrII
BbrPI BbsI BciVI BclI BfrI
BlnI BpuAI BsaAI BsaBI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
BspEI BspHI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HgaI HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MseI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 889 GTCTAC GT^[AC][GT]AC
AcyI 538 GGCGCC G[GA]^CG[CT]C
AocI 1260 CCTGAGG CC^T[ACGT]AGG
Asp700I 639 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 26 TGCGCA TGC^GCA
BamHI 911 GGATCC G^GATCC
BanI 538 GGCGCC G^G[CT][GA]CC
BanII 4 GAGCCC G[GA]GC[CT]^C
BbeI 538 GGCGCC GGCGC^C
BbvCI 368 CCTCAGC CCTCAGC(-5/-2)
BcgI 1332 CGACAGGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1434 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1434 GCTCAGC GC^T[ACGT]AGC
BsaHI 538 GGCGCC G[GA]^CG[CT]C
BsaWI 908 ACCGGA [AT]^CCGG[AT]
Bsp1407I 361 TGTACA T^GTACA
BspDI 1450 ATCGAT AT^CGAT
BsrFI 1130 ACCGGC [GA]^CCGG[CT]
BsrGI 361 TGTACA T^GTACA
BssSI 1309 CACGAG CACGAG(-5/-1)
BstAPI 18 GCACGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1260 CCTGAGG CC^T[ACGT]AGG
CelII 1434 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1130 ACCGGC [GA]^CCGG[CT]
ClaI 1450 ATCGAT AT^CGAT
EarI 460 GAAGAG CTCTTC(1/4)
Eco81I 1260 CCTGAGG CC^T[ACGT]AGG
FspI 26 TGCGCA TGC^GCA
HaeII 538 GGCGCC [GA]GCGC^[CT]
Hin1I 538 GGCGCC G[GA]^CG[CT]C
HincII 1156 GTCAAC GT[CT]^[GA]AC
HindII 1156 GTCAAC GT[CT]^[GA]AC
KasI 538 GGCGCC G^GCGCC
Ksp632I 460 GAAGAG CTCTTC(1/4)
MaeII 950 ACGT A^CGT
MaeIII 124 GTGAC ^GT[ACGT]AC
NarI 538 GGCGCC GG^CGCC
NcoI 587 CCATGG C^CATGG
NspI 551 ACATGC [GA]CATG^[CT]
PflMI 1044 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 374 CTGCAG CTGCA^G
SexAI 213 ACCAGGT A^CC[AT]GGT
SfoI 538 GGCGCC GGC^GCC
SmaI 7 CCCGGG CCC^GGG
SspBI 361 TGTACA T^GTACA
Tsp45I 124 GTGAC ^GT[GC]AC
Van91I 1044 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 328 CCACCAACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 7 CCCGGG C^CCGGG
XmaI 7 CCCGGG C^CCGGG
XmnI 639 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AhdI 934 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1272 GACACTATGTC
AlwNI 245 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1423 CAGGCACTG
AspEI 934 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1272 GACACTATGTC
BsrI 1113 ACTGG ACTGG(1/-1)
1428 ACTGG
Eam1105I 934 GACCCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1272 GACACTATGTC
HphI 115 TCACC GGTGA(8/7)
1206 TCACC
MboII 460 GAAGA GAAGA(8/7)
614 TCTTC
PleI 253 GAGTC GAGTC(4/5)
1289 GAGTC
SfcI 374 CTGCAG C^T[GA][CT]AG
759 CTACAG
TfiI 143 GAATC G^A[AT]TC
1203 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BalI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeI MboI MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StuI StyI TaqI TseI TspRI
TthHB8I XhoII