Restriction map for
pFN21AE5633
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BbeI
BbvCI BcgI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I CelII ClaI DraI
Eam1105I EcoNI EcoRI EcoRV FbaI
FseI Hin1I HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI PvuI SacI SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AclI 570 AACGTT AA^CGTT
AcsI 885 GAATTT [GA]^AATT[CT]
AfaI 369 GTAC GT^AC
AlwNI 296 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1442 AGCGCT AGC^GCT
ApoI 885 GAATTT [GA]^AATT[CT]
Asp700I 298 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 838 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 944 CCCGGG C^[CT]CG[GA]G
BalI 777 TGGCCA TGG^CCA
BanII 1273 GAGCCC G[GA]GC[CT]^C
BbrPI 313 CACGTG CAC^GTG
BbsI 1105 GTCTTC GAAGAC(2/6)
BciVI 1394 GTATCC GTATCC(6/5)
BfaI 911 CTAG C^TAG
BglII 1071 AGATCT A^GATCT
BpuAI 1105 GTCTTC GAAGAC(2/6)
BsgI 1045 GTGCAG GTGCAG(16/14)
BsoBI 944 CCCGGG C^[CT]CG[GA]G
BspHI 647 TCATGA T^CATGA
BsrDI 465 CATTGC GCAATG(2/0)
BsrFI 811 ACCGGC [GA]^CCGG[CT]
BstAPI 1374 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 789 GGTCACC G^GT[ACGT]ACC
BstPI 789 GGTCACC G^GT[ACGT]ACC
BstXI 587 CCAGCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 811 ACCGGC [GA]^CCGG[CT]
CpoI 1384 CGGACCG CG^G[AT]CCG
DraIII 141 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 355 GACAGAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 481 CGGCCG C^GGCCG
EarI 1054 CTCTTC CTCTTC(1/4)
EclXI 481 CGGCCG C^GGCCG
Eco47III 1442 AGCGCT AGC^GCT
Eco52I 481 CGGCCG C^GGCCG
EcoO65I 789 GGTCACC G^GT[ACGT]ACC
EcoT22I 1235 ATGCAT ATGCA^T
HaeII 1442 AGCGCT [GA]GCGC^[CT]
HgaI 167 GCGTC GACGC(5/10)
Ksp632I 1054 CTCTTC CTCTTC(1/4)
KspI 669 CCGCGG CCGC^GG
MaeI 911 CTAG C^TAG
MluNI 777 TGGCCA TGG^CCA
MscI 777 TGGCCA TGG^CCA
MseI 374 TTAA T^TAA
NcoI 695 CCATGG C^CATGG
NsiI 1235 ATGCAT ATGCA^T
PflFI 838 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 313 CACGTG CAC^GTG
PmlI 313 CACGTG CAC^GTG
PpuMI 1063 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 570 AACGTT AA^CGTT
PstI 784 CTGCAG CTGCA^G
RcaI 647 TCATGA T^CATGA
RsaI 369 GTAC GT^AC
RsrII 1384 CGGACCG CG^G[AT]CCG
SacII 669 CCGCGG CCGC^GG
SapI 1053 GCTCTTC GCTCTTC(1/4)
ScaI 368 AGTACT AGT^ACT
SfcI 784 CTGCAG C^T[GA][CT]AG
SmaI 944 CCCGGG CCC^GGG
SmlI 1331 CTCAAG C^T[CT][GA]AG
Tru9I 374 TTAA T^TAA
Tsp509I 886 AATT ^AATT
Tth111I 838 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 944 CCCGGG C^CCGGG
XmaI 944 CCCGGG C^CCGGG
XmnI 298 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 670 CGCG CG^CG
755 CGCG
AocI 735 CCTGAGG CC^T[ACGT]AGG
1266 CCTGAGG
AspHI 400 GAGCAC G[AT]GC[AT]^C
706 GAGCAC
BglI 73 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
152 GCCTCGGCGGC
BsaAI 118 TACGTG [CT]AC^GT[GA]
313 CACGTG
BsiEI 481 CGGCCG CG[GA][CT]^CG
1100 CGACCG
BsiHKAI 400 GAGCAC G[AT]GC[AT]^C
706 GAGCAC
BsmFI 23 GGGAC GGGAC(10/14)
865 GTCCC
BstUI 670 CGCG CG^CG
755 CGCG
Bsu36I 735 CCTGAGG CC^T[ACGT]AGG
1266 CCTGAGG
DraII 149 GGGGCCT [GA]G^G[ACGT]CC[CT]
1063 GGGTCCT
Eco81I 735 CCTGAGG CC^T[ACGT]AGG
1266 CCTGAGG
EcoO109I 149 GGGGCCT [GA]G^G[ACGT]CC[CT]
1063 GGGTCCT
HincII 169 GTCAAC GT[CT]^[GA]AC
394 GTTGAC
HindII 169 GTCAAC GT[CT]^[GA]AC
394 GTTGAC
MvnI 670 CGCG CG^CG
755 CGCG
NspI 1176 GCATGT [GA]CATG^[CT]
1405 ACATGC
PleI 940 GACTC GAGTC(4/5)
863 GAGTC
StuI 222 AGGCCT AGG^CCT
1449 AGGCCT
XcmI 977 CCATTGGTGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1016 CCACAGCGATCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI AlwI AvaII AviII
BanI BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII