Restriction map for
pFN21AE5770
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI AocI ApaI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BanI BbeI BbrPI BciVI
BclI BfaI BlnI BlpI Bpu1102I
BsaAI BsaBI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspHI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI ScaI SfiI
SfoI SfuI SnaBI SpeI SphI
SspBI SspI SwaI Van91I XbaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 66 CGCG CG^CG
AcyI 96 GACGCC G[GA]^CG[CT]C
AfaI 90 GTAC GT^AC
AflII 1690 CTTAAG C^TTAAG
AflIII 440 ACATGT A^C[GA][CT]GT
Alw44I 969 GTGCAC G^TGCAC
Aor51HI 1099 AGCGCT AGC^GCT
ApaLI 969 GTGCAC G^TGCAC
AspHI 969 GTGCAC G[AT]GC[AT]^C
AspI 295 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 18 GGGCTC G[GA]GC[CT]^C
BbvCI 1371 GCTGAGG CCTCAGC(-5/-2)
BcgI 173 GCAAAACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1690 CTTAAG C^TTAAG
BglI 1088 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 926 AGATCT A^GATCT
BsaHI 96 GACGCC G[GA]^CG[CT]C
BsaI 666 GGTCTC GGTCTC(1/5)
BseRI 585 GAGGAG GAGGAG(10/8)
BsiEI 1068 CGGTCG CG[GA][CT]^CG
BsiHKAI 969 GTGCAC G[AT]GC[AT]^C
BsmAI 667 GTCTC GTCTC(1/5)
BspLU11I 440 ACATGT A^CATGT
BspMI 54 GCAGGT ACCTGC(4/8)
BsrBI 641 GAGCGG CCGCTC(-3/-3)
BsrDI 1040 GCAATG GCAATG(2/0)
BsrFI 99 GCCGGT [GA]^CCGG[CT]
BssHII 65 GCGCGC G^CGCGC
BstUI 66 CGCG CG^CG
BstXI 1226 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 99 GCCGGT [GA]^CCGG[CT]
DraI 1456 TTTAAA TTT^AAA
DraII 299 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco47III 1099 AGCGCT AGC^GCT
EcoO109I 299 AGGTCCT [GA]G^G[ACGT]CC[CT]
HaeII 1099 AGCGCT [GA]GCGC^[CT]
Hin1I 96 GACGCC G[GA]^CG[CT]C
MaeII 1433 ACGT A^CGT
MvnI 66 CGCG CG^CG
NdeI 1280 CATATG CA^TATG
PflFI 295 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 299 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 90 GTAC GT^AC
SbfI 1170 CCTGCAGG CCTGCA^GG
SgrAI 98 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 986 CCCGGG CCC^GGG
Sse8387I 1170 CCTGCAGG CCTGCA^GG
StuI 594 AGGCCT AGG^CCT
Tth111I 295 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 269 CCACTCGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 986 CCCGGG C^CCGGG
XmaI 986 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 446 TCCGGA T^CCGGA
650 TCCGGA
AlwNI 55 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
822 CAGCACCTG
BbsI 708 GAAGAC GAAGAC(2/6)
814 GTCTTC
BpuAI 708 GAAGAC GAAGAC(2/6)
814 GTCTTC
BsaWI 446 TCCGGA [AT]^CCGG[AT]
650 TCCGGA
BseAI 446 TCCGGA T^CCGGA
650 TCCGGA
BsgI 46 CTGCAC GTGCAG(16/14)
103 GTGCAG
BsmI 1306 GAATGC GAATGC(1/-1)
893 GCATTC
BspEI 446 TCCGGA T^CCGGA
650 TCCGGA
BsrI 1013 ACTGG ACTGG(1/-1)
1741 ACTGG
EcoT14I 1121 CCATGG C^C[AT][AT]GG
1226 CCATGG
HgaI 96 GACGC GACGC(5/10)
615 GACGC
HincII 1009 GTCAAC GT[CT]^[GA]AC
1734 GTTGAC
HindII 1009 GTCAAC GT[CT]^[GA]AC
1734 GTTGAC
MaeIII 10 GTGAC ^GT[ACGT]AC
160 GTCAC
MroI 446 TCCGGA T^CCGGA
650 TCCGGA
MslI 408 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
972 CACTGATGTG
NcoI 1121 CCATGG C^CATGG
1226 CCATGG
NspI 6 GCATGT [GA]CATG^[CT]
440 ACATGT
PaeR7I 272 CTCGAG C^TCGAG
1355 CTCGAG
PstI 149 CTGCAG CTGCA^G
1171 CTGCAG
PvuII 477 CAGCTG CAG^CTG
769 CAGCTG
SexAI 632 ACCTGGT A^CC[AT]GGT
809 ACCTGGT
SfcI 149 CTGCAG C^T[GA][CT]AG
1171 CTGCAG
StyI 1121 CCATGG C^C[AT][AT]GG
1226 CCATGG
TaqI 273 TCGA T^CGA
1356 TCGA
Tsp45I 10 GTGAC ^GT[GC]AC
160 GTCAC
TthHB8I 273 TCGA T^CGA
1356 TCGA
XhoI 272 CTCGAG C^TCGAG
1355 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I AvaI AvaII BalI BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsoBI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SmlI
TfiI Tru9I TseI Tsp509I TspRI
XhoII XmnI