Restriction map for
pFN21AB9597
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I AspI
AviII AvrII BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII EarI EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI Ksp632I
MaeI MaeIII MamI MboII MfeI
MluI MroI MseI MunI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp45I Tsp509I TspRI Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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Acc65I 933 GGTACC G^GTACC
AflIII 626 ACATGT A^C[GA][CT]GT
AhdI 610 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 662 AGCGCT AGC^GCT
Asp718I 933 GGTACC G^GTACC
AspEI 610 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 753 GGATCC G^GATCC
BglII 455 AGATCT A^GATCT
BsgI 1199 GTGCAG GTGCAG(16/14)
BspLU11I 626 ACATGT A^CATGT
BsrBI 914 CCGCTC CCGCTC(-3/-3)
BsrI 599 ACTGG ACTGG(1/-1)
BssSI 166 CACGAG CACGAG(-5/-1)
DrdI 547 GACTGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 610 GACCCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 662 AGCGCT AGC^GCT
EcoNI 1021 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 410 CCATGG C^C[AT][AT]GG
FseI 848 GGCCGGCC GGCCGG^CC
KpnI 933 GGTACC GGTAC^C
NcoI 410 CCATGG C^CATGG
NspI 626 ACATGT [GA]CATG^[CT]
PshAI 338 GACCGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 421 CTGCAG CTGCA^G
SfiI 1048 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1293 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1177 CTCAAG C^T[CT][GA]AG
StyI 410 CCATGG C^C[AT][AT]GG
TaqI 632 TCGA T^CGA
TthHB8I 632 TCGA T^CGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 393 GTAC GT^AC
934 GTAC
AocI 1025 CCTCAGG CC^T[ACGT]AGG
1439 CCTCAGG
BseRI 211 GAGGAG GAGGAG(10/8)
214 GAGGAG
BstAPI 11 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
675 GCAGCCCCTGC
Bsu36I 1025 CCTCAGG CC^T[ACGT]AGG
1439 CCTCAGG
Eco81I 1025 CCTCAGG CC^T[ACGT]AGG
1439 CCTCAGG
MslI 78 CATAGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
405 CATCACCATG
NotI 271 GCGGCCGC GC^GGCCGC
473 GCGGCCGC
PflMI 1251 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1353 CCAGGTCTTGG
PpuMI 1335 GGGTCCT [GA]G^G[AT]CC[CT]
1444 GGGTCCC
PvuII 12 CAGCTG CAG^CTG
925 CAGCTG
RsaI 393 GTAC GT^AC
934 GTAC
SacI 42 GAGCTC GAGCT^C
112 GAGCTC
SfcI 421 CTGCAG C^T[GA][CT]AG
1244 CTATAG
StuI 1056 AGGCCT AGG^CCT
1231 AGGCCT
Van91I 1251 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1353 CCAGGTCTTGG
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
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AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcnI BglI BmyI BsaHI BsaJI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KspI MaeII MboI
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PleI SacII Sau3AI Sau96I
ScrFI SfaNI SfoI SmaI TseI
XhoII XmaCI XmaI