Restriction map for
pFN21AE5083
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# NO CUT ENZYMES ( 80 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AscI AseI AsnI Asp700I Asp718I
AspI AviII BamHI BbrPI BcgI
BfrI BlpI Bpu1102I BsaAI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspEI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I CelII CpoI DrdI
EagI EclXI Eco52I EcoNI EcoRI
FseI FspI HincII HindII HindIII
HpaI KpnI MfeI MluI MroI
MslI MunI NcoI NheI NotI
NruI NspV PacI PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SalI SbfI SfiI
SfuI SgrAI SnaBI Sse8387I SspBI
SspI SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 1949 GACGTC GACGT^C
AccI 1570 GTAGAC GT^[AC][GT]AC
AfaI 1241 GTAC GT^AC
AgeI 340 ACCGGT A^CCGGT
AhdI 2602 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2255 GTGCAC G^TGCAC
AlwI 2043 GGATC GGATC(4/5)
AocI 1933 CCTGAGG CC^T[ACGT]AGG
Aor51HI 146 AGCGCT AGC^GCT
ApaI 2557 GGGCCC GGGCC^C
ApaLI 2255 GTGCAC G^TGCAC
AspEI 2602 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1915 CCTAGG C^CTAGG
BalI 2819 TGGCCA TGG^CCA
BbeI 761 GGCGCC GGCGC^C
BciVI 901 GGATAC GTATCC(6/5)
BglI 2323 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1890 AGATCT A^GATCT
BlnI 1915 CCTAGG C^CTAGG
BspMI 1057 GCAGGT ACCTGC(4/8)
BssSI 1415 CACGAG CACGAG(-5/-1)
BstYI 1890 AGATCT [GA]^GATC[CT]
Bsu36I 1933 CCTGAGG CC^T[ACGT]AGG
DraI 2682 TTTAAA TTT^AAA
Eam1105I 2602 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 146 AGCGCT AGC^GCT
Eco81I 1933 CCTGAGG CC^T[ACGT]AGG
EcoRV 1210 GATATC GAT^ATC
EcoT22I 2407 ATGCAT ATGCA^T
KasI 761 GGCGCC G^GCGCC
MflI 1890 AGATCT [GA]^GATC[CT]
MluNI 2819 TGGCCA TGG^CCA
MscI 2819 TGGCCA TGG^CCA
NaeI 758 GCCGGC GCC^GGC
NarI 761 GGCGCC GG^CGCC
NdeI 1525 CATATG CA^TATG
NgoMIV 758 GCCGGC G^CCGGC
NsiI 2407 ATGCAT ATGCA^T
PaeR7I 971 CTCGAG C^TCGAG
PflMI 1540 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 340 ACCGGT A^CCGGT
PshAI 2014 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1241 GTAC GT^AC
ScaI 1240 AGTACT AGT^ACT
SexAI 2840 ACCTGGT A^CC[AT]GGT
SfoI 761 GGCGCC GGC^GCC
SmaI 431 CCCGGG CCC^GGG
SpeI 1237 ACTAGT A^CTAGT
Van91I 1540 CCACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 971 CTCGAG C^TCGAG
XhoII 1890 AGATCT [GA]^GATC[CT]
XmaCI 431 CCCGGG C^CCGGG
XmaI 431 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanII 225 GGGCTC G[GA]GC[CT]^C
2557 GGGCCC
BbvCI 473 CCTCAGC CCTCAGC(-5/-2)
2621 CCTCAGC
BsaBI 790 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1885 GATCGAGATC
BsmBI 2500 CGTCTC CGTCTC(1/5)
107 GAGACG
BspDI 1427 ATCGAT AT^CGAT
2066 ATCGAT
BspHI 573 TCATGA T^CATGA
2846 TCATGA
BstEII 835 GGTCACC G^GT[ACGT]ACC
2422 GGTGACC
BstPI 835 GGTCACC G^GT[ACGT]ACC
2422 GGTGACC
BstXI 2573 CCATTCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2810 CCAAGGCACTGG
ClaI 1427 ATCGAT AT^CGAT
2066 ATCGAT
DraIII 1541 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2771 CACCTTGTG
EcoO65I 835 GGTCACC G^GT[ACGT]ACC
2422 GGTGACC
MamI 790 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1885 GATCGAGATC
MseI 1168 TTAA T^TAA
2683 TTAA
PvuII 2171 CAGCTG CAG^CTG
2624 CAGCTG
RcaI 573 TCATGA T^CATGA
2846 TCATGA
SapI 2130 GAAGAGC GCTCTTC(1/4)
1125 GCTCTTC
StuI 1219 AGGCCT AGG^CCT
1380 AGGCCT
Tru9I 1168 TTAA T^TAA
2683 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwNI ApoI AspHI AvaI AvaII
BanI BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI