Restriction map for
pFN21AE5563
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI AocI Aor51HI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvI
BcgI BciVI BcnI BfaI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FauI
Fnu4HI FseI FspI HaeII HhaI
HinP1I HindIII HpaI HphI ItaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TseI
Tsp45I Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 418 GACGTC GACGT^C
AccII 503 CGCG CG^CG
AcyI 418 GACGTC G[GA]^CG[CT]C
AflII 39 CTTAAG C^TTAAG
AflIII 891 ACATGT A^C[GA][CT]GT
Alw44I 1183 GTGCAC G^TGCAC
AlwNI 1223 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 663 GGGCCC GGGCC^C
ApaLI 1183 GTGCAC G^TGCAC
AseI 604 ATTAAT AT^TAAT
AsnI 604 ATTAAT AT^TAAT
BamHI 480 GGATCC G^GATCC
BanII 663 GGGCCC G[GA]GC[CT]^C
BfrI 39 CTTAAG C^TTAAG
BpmI 935 CTCCAG CTGGAG(16/14)
BsaHI 418 GACGTC G[GA]^CG[CT]C
BsaI 404 GGTCTC GGTCTC(1/5)
BsgI 1031 GTGCAG GTGCAG(16/14)
BsmFI 416 GGGAC GGGAC(10/14)
BspLU11I 891 ACATGT A^CATGT
BstAPI 1118 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 854 TTCGAA TT^CGAA
BstUI 503 CGCG CG^CG
EaeI 778 TGGCCG [CT]^GGCC[GA]
EarI 272 GAAGAG CTCTTC(1/4)
HapII 781 CCGG C^CGG
HgaI 504 GCGTC GACGC(5/10)
Hin1I 418 GACGTC G[GA]^CG[CT]C
HincII 1079 GTCAAC GT[CT]^[GA]AC
HindII 1079 GTCAAC GT[CT]^[GA]AC
HpaII 781 CCGG C^CGG
Ksp632I 272 GAAGAG CTCTTC(1/4)
MaeIII 580 GTAAC ^GT[ACGT]AC
MslI 1024 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 781 CCGG C^CGG
MvnI 503 CGCG CG^CG
NspI 891 ACATGT [GA]CATG^[CT]
NspV 854 TTCGAA TT^CGAA
PflMI 123 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 933 GACTC GAGTC(4/5)
PshBI 604 ATTAAT AT^TAAT
SfaNI 1108 GATGC GCATC(5/9)
SfuI 854 TTCGAA TT^CGAA
TaqI 855 TCGA T^CGA
TthHB8I 855 TCGA T^CGA
Van91I 123 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 215 CCGC CCGC(-3/-1)
502 CCGC
AfaI 157 GTAC GT^AC
909 GTAC
Asp700I 22 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
642 GAAAGCCTTC
BbsI 712 GAAGAC GAAGAC(2/6)
982 GAAGAC
BbvCI 549 CCTCAGC CCTCAGC(-5/-2)
411 GCTGAGG
BpuAI 712 GAAGAC GAAGAC(2/6)
982 GAAGAC
BsiYI 123 CCACAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
502 CCGCGTCATGG
BslI 123 CCACAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
502 CCGCGTCATGG
BsmAI 1225 GAGAC GTCTC(1/5)
405 GTCTC
BsrDI 651 CATTGC GCAATG(2/0)
364 GCAATG
BssKI 63 CCAGG ^CC[ACGT]GG
999 CCAGG
BstNI 63 CCAGG CC^[AT]GG
999 CCAGG
BstYI 68 AGATCC [GA]^GATC[CT]
480 GGATCC
DsaI 349 CCACGG C^C[GA][CT]GG
680 CCGTGG
EcoRI 392 GAATTC G^AATTC
451 GAATTC
EcoRII 63 CCAGG ^CC[AT]GG
999 CCAGG
MaeII 284 ACGT A^CGT
419 ACGT
MflI 68 AGATCC [GA]^GATC[CT]
480 GGATCC
MspA1I 409 CAGCTG C[AC]G^C[GT]G
1084 CAGCTG
MvaI 63 CCAGG CC^[AT]GG
999 CCAGG
PstI 376 CTGCAG CTGCA^G
1026 CTGCAG
PvuII 409 CAGCTG CAG^CTG
1084 CAGCTG
RsaI 157 GTAC GT^AC
909 GTAC
ScrFI 63 CCAGG CC^[ACGT]GG
999 CCAGG
SmlI 39 CTTAAG C^T[CT][GA]AG
179 CTCAAG
TfiI 3 GATTC G^A[AT]TC
586 GATTC
XhoII 68 AGATCC [GA]^GATC[CT]
480 GGATCC
XmnI 22 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
642 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AluI AlwI ApoI AspHI
AvaII BclI BmyI BsaJI BsiHKAI
Bsp1286I BsrI BstF5I Cac8I Cfr13I
DdeI DpnII DraII EcoO109I FbaI
FokI HaeIII HinfI MboI MboII
MnlI MseI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I SfcI Tru9I
Tsp509I TspRI