Restriction map for
pFN21AE5080
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssKI BssSI Bst1107I
BstBI BstEII BstF5I BstNI BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRII
EcoRV FauI FbaI FokI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvaI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AfaI 360 GTAC GT^AC
AlwNI 541 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 574 ATTAAT AT^TAAT
AsnI 574 ATTAAT AT^TAAT
Asp700I 916 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 637 GTGCTC G[AT]GC[AT]^C
BalI 329 TGGCCA TGG^CCA
BamHI 402 GGATCC G^GATCC
BanII 460 GGGCTC G[GA]GC[CT]^C
BbsI 703 GTCTTC GAAGAC(2/6)
BbvCI 685 GCTGAGG CCTCAGC(-5/-2)
BfaI 553 CTAG C^TAG
BpuAI 703 GTCTTC GAAGAC(2/6)
BseRI 121 CTCCTC GAGGAG(10/8)
BsiHKAI 637 GTGCTC G[AT]GC[AT]^C
BsmBI 154 CGTCTC CGTCTC(1/5)
BsmFI 226 GTCCC GGGAC(10/14)
BsrDI 237 CATTGC GCAATG(2/0)
BstAPI 586 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 402 GGATCC [GA]^GATC[CT]
DraI 180 TTTAAA TTT^AAA
DraII 449 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 143 CCGTGG C^C[GA][CT]GG
EaeI 329 TGGCCA [CT]^GGCC[GA]
EcoO109I 449 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 568 GAATTC G^AATTC
EcoT14I 698 CCAAGG C^C[AT][AT]GG
HapII 693 CCGG C^CGG
HincII 374 GTCAAC GT[CT]^[GA]AC
HindII 374 GTCAAC GT[CT]^[GA]AC
HindIII 58 AAGCTT A^AGCTT
HpaII 693 CCGG C^CGG
MaeI 553 CTAG C^TAG
MaeII 604 ACGT A^CGT
MflI 402 GGATCC [GA]^GATC[CT]
MluNI 329 TGGCCA TGG^CCA
MscI 329 TGGCCA TGG^CCA
MspI 693 CCGG C^CGG
PleI 509 GAGTC GAGTC(4/5)
PshBI 574 ATTAAT AT^TAAT
RsaI 360 GTAC GT^AC
SapI 916 GAAGAGC GCTCTTC(1/4)
StyI 698 CCAAGG C^C[AT][AT]GG
TaqI 796 TCGA T^CGA
TthHB8I 796 TCGA T^CGA
XcmI 134 CCATCATTACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 402 GGATCC [GA]^GATC[CT]
XmnI 916 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 243 AAATTC [GA]^AATT[CT]
568 GAATTC
AlwI 403 GATCC GGATC(4/5)
402 GGATC
ApoI 243 AAATTC [GA]^AATT[CT]
568 GAATTC
BmyI 460 GGGCTC G[AGT]GC[ACT]^C
637 GTGCTC
BsaJI 143 CCGTGG C^C[ACGT][ACGT]GG
698 CCAAGG
BsmAI 391 GAGAC GTCTC(1/5)
155 GTCTC
BsmI 284 GCATTC GAATGC(1/-1)
595 GCATTC
Bsp1286I 460 GGGCTC G[AGT]GC[ACT]^C
637 GTGCTC
BspMI 98 ACCTGC ACCTGC(4/8)
712 ACCTGC
DpnII 403 GATC ^GATC
627 GATC
EcoT22I 74 ATGCAT ATGCA^T
466 ATGCAT
MboI 403 GATC ^GATC
627 GATC
MslI 138 CATTACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
339 CACAGTGATG
MspA1I 683 CAGCTG C[AC]G^C[GT]G
752 CAGCTG
MwoI 424 GCAGTGATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
586 GCACTGGCTGC
NdeII 403 GATC ^GATC
627 GATC
NlaIV 402 GGATCC GG[ACGT]^[ACGT]CC
449 GGGGCC
NsiI 74 ATGCAT ATGCA^T
466 ATGCAT
PshAI 148 GACCGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
696 GACCAAGGTC
PstI 608 CTGCAG CTGCA^G
755 CTGCAG
PvuII 683 CAGCTG CAG^CTG
752 CAGCTG
Sau3AI 403 GATC ^GATC
627 GATC
SphI 420 GCATGC GCATG^C
468 GCATGC
Tsp45I 91 GTGAC ^GT[GC]AC
220 GTCAC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI AvaII BbvI BsiYI BslI
BsrI Cac8I Cfr13I DdeI EarI
Fnu4HI HaeIII HinfI HphI ItaI
Ksp632I MaeIII MboII MnlI MseI
NlaIII NspI Sau96I SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI