Restriction map for
pFN21AE3198
##################################
# NO CUT ENZYMES ( 149 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaBI BsaI BseAI
BsgI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BsrI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstZ17I Bsu36I CelII ClaI DdeI
DraI DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeI MaeIII MamI MboII
MfeI MluI MroI MseI MslI
MunI NcoI NdeI NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 424 GACGTC GACGT^C
AfaI 99 GTAC GT^AC
ApaI 211 GGGCCC GGGCC^C
AspHI 312 GAGCTC G[AT]GC[AT]^C
BamHI 326 GGATCC G^GATCC
BbrPI 106 CACGTG CAC^GTG
BglI 250 GCCCGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 106 CACGTG [CT]AC^GT[GA]
BsaWI 378 ACCGGA [AT]^CCGG[AT]
BseRI 260 CTCCTC GAGGAG(10/8)
BsiHKAI 312 GAGCTC G[AT]GC[AT]^C
BsrBI 19 GAGCGG CCGCTC(-3/-3)
BsrFI 38 GCCGGC [GA]^CCGG[CT]
BssSI 276 CTCGTG CACGAG(-5/-1)
BstYI 326 GGATCC [GA]^GATC[CT]
Cfr10I 38 GCCGGC [GA]^CCGG[CT]
CpoI 486 CGGACCG CG^G[AT]CCG
DpnII 327 GATC ^GATC
DraII 211 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 127 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 43 CCGCGG C^C[GA][CT]GG
EcoO109I 211 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 462 CCTTGG C^C[AT][AT]GG
HphI 172 TCACC GGTGA(8/7)
KspI 43 CCGCGG CCGC^GG
MboI 327 GATC ^GATC
MflI 326 GGATCC [GA]^GATC[CT]
NaeI 38 GCCGGC GCC^GGC
NdeII 327 GATC ^GATC
NgoMIV 38 GCCGGC G^CCGGC
NlaIII 156 CATG CATG^
NotI 202 GCGGCCGC GC^GGCCGC
PflMI 126 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 106 CACGTG CAC^GTG
PmlI 106 CACGTG CAC^GTG
RsaI 99 GTAC GT^AC
RsrII 486 CGGACCG CG^G[AT]CCG
SacI 312 GAGCTC GAGCT^C
SacII 43 CCGCGG CCGC^GG
Sau3AI 327 GATC ^GATC
StyI 462 CCTTGG C^C[AT][AT]GG
TspRI 53 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 126 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 326 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 327 GATCC GGATC(4/5)
326 GGATC
AlwNI 286 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
362 CAGAGCCTG
AvaII 444 GGACC G^G[AT]CC
487 GGACC
BalI 218 TGGCCA TGG^CCA
405 TGGCCA
BanI 8 GGCGCC G^G[CT][GA]CC
185 GGCGCC
BbeI 8 GGCGCC GGCGC^C
185 GGCGCC
BsiEI 40 CGGCCG CG[GA][CT]^CG
203 CGGCCG
EagI 40 CGGCCG C^GGCCG
203 CGGCCG
EclXI 40 CGGCCG C^GGCCG
203 CGGCCG
Eco52I 40 CGGCCG C^GGCCG
203 CGGCCG
HaeII 8 GGCGCC [GA]GCGC^[CT]
185 GGCGCC
HinfI 114 GAGTC G^A[ACGT]TC
382 GAGTC
KasI 8 GGCGCC G^GCGCC
185 GGCGCC
MaeII 107 ACGT A^CGT
425 ACGT
MluNI 218 TGGCCA TGG^CCA
405 TGGCCA
MscI 218 TGGCCA TGG^CCA
405 TGGCCA
NarI 8 GGCGCC GG^CGCC
185 GGCGCC
PleI 114 GAGTC GAGTC(4/5)
382 GAGTC
SfoI 8 GGCGCC GGC^GCC
185 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AcyI AluI BanII
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr13I EaeI EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TseI