Restriction map for
pFN21AE5072
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HgaI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI Ksp632I MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AluI 154 AGCT AG^CT
Alw44I 277 GTGCAC G^TGCAC
AlwNI 85 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 136 GGGCCC GGGCC^C
ApaLI 277 GTGCAC G^TGCAC
AvaII 96 GGACC G^G[AT]CC
AviII 182 TGCGCA TGC^GCA
BanII 136 GGGCCC G[GA]GC[CT]^C
BbsI 424 GTCTTC GAAGAC(2/6)
BglI 299 GCCGCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 424 GTCTTC GAAGAC(2/6)
BsaI 400 GAGACC GGTCTC(1/5)
BsiEI 222 CGACCG CG[GA][CT]^CG
BsmAI 400 GAGAC GTCTC(1/5)
BsrBI 337 GAGCGG CCGCTC(-3/-3)
BsrDI 363 CATTGC GCAATG(2/0)
BstF5I 186 CATCC GGATG(2/0)
DraII 136 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 112 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 64 CCGCGG C^C[GA][CT]GG
EaeI 297 TGGCCG [CT]^GGCC[GA]
EcoNI 327 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 136 GGGCCCC [GA]G^G[ACGT]CC[CT]
FokI 186 CATCC GGATG(9/13)
FspI 182 TGCGCA TGC^GCA
HaeII 57 GGCGCT [GA]GCGC^[CT]
KspI 64 CCGCGG CCGC^GG
MaeIII 323 GTTAC ^GT[ACGT]AC
MboII 425 TCTTC GAAGA(8/7)
NlaIII 312 CATG CATG^
PstI 350 CTGCAG CTGCA^G
PvuII 153 CAGCTG CAG^CTG
SacII 64 CCGCGG CCGC^GG
SfcI 350 CTGCAG C^T[GA][CT]AG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 65 CGCG CG^CG
133 CGCG
AspHI 277 GTGCAC G[AT]GC[AT]^C
284 GTGCTC
BanI 110 GGCACC G^G[CT][GA]CC
307 GGCACC
BsiHKAI 277 GTGCAC G[AT]GC[AT]^C
284 GTGCTC
BsmFI 51 GGGAC GGGAC(10/14)
120 GTCCC
BsrFI 41 GCCGGC [GA]^CCGG[CT]
204 GCCGGC
BstAPI 174 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
352 GCAGGAGATGC
BstUI 65 CGCG CG^CG
133 CGCG
Cfr10I 41 GCCGGC [GA]^CCGG[CT]
204 GCCGGC
DpnII 219 GATC ^GATC
237 GATC
HphI 330 GGTGA GGTGA(8/7)
167 TCACC
MboI 219 GATC ^GATC
237 GATC
MspA1I 64 CCGCGG C[AC]G^C[GT]G
153 CAGCTG
MvnI 65 CGCG CG^CG
133 CGCG
NaeI 41 GCCGGC GCC^GGC
204 GCCGGC
NdeII 219 GATC ^GATC
237 GATC
NgoMIV 41 GCCGGC G^CCGGC
204 GCCGGC
Sau3AI 219 GATC ^GATC
237 GATC
SexAI 113 ACCTGGT A^CC[AT]GGT
326 ACCTGGT
SfaNI 358 GATGC GCATC(5/9)
185 GCATC
TaqI 221 TCGA T^CGA
398 TCGA
TspRI 280 CACTG CA[GC]TG[ACGT][ACGT]^
303 CACTG
TthHB8I 221 TCGA T^CGA
398 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI BbvI BcnI BmyI BsaJI
BsgI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cac8I CfoI Cfr13I
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MnlI
MspI MvaI MwoI NciI NlaIV
Sau96I ScrFI TseI