Restriction map for
pFN21AE5060
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII AccI AccII AclI AcyI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AspEI
AspHI AspI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BcnI BfaI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaHI
BsaI BseRI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KspI MaeI
MfeI MluI MslI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 686 TCCGGA T^CCGGA
AflIII 385 ACGTGT A^C[GA][CT]GT
Asp700I 49 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 302 CTCGGG C^[CT]CG[GA]G
BalI 425 TGGCCA TGG^CCA
BbsI 949 GTCTTC GAAGAC(2/6)
BclI 735 TGATCA T^GATCA
BpmI 644 CTGGAG CTGGAG(16/14)
BpuAI 949 GTCTTC GAAGAC(2/6)
BsaWI 686 TCCGGA [AT]^CCGG[AT]
BseAI 686 TCCGGA T^CCGGA
BsgI 21 GTGCAG GTGCAG(16/14)
BsmBI 64 GAGACG CGTCTC(1/5)
BsoBI 302 CTCGGG C^[CT]CG[GA]G
BspEI 686 TCCGGA T^CCGGA
BsrDI 1095 CATTGC GCAATG(2/0)
BstYI 306 GGATCT [GA]^GATC[CT]
DraI 799 TTTAAA TTT^AAA
DraII 923 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 425 TGGCCA [CT]^GGCC[GA]
EarI 1075 GAAGAG CTCTTC(1/4)
EcoO109I 923 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 496 GAATTC G^AATTC
EcoT14I 862 CCAAGG C^C[AT][AT]GG
EcoT22I 207 ATGCAT ATGCA^T
FbaI 735 TGATCA T^GATCA
HapII 687 CCGG C^CGG
HincII 34 GTTGAC GT[CT]^[GA]AC
HindII 34 GTTGAC GT[CT]^[GA]AC
HpaII 687 CCGG C^CGG
Ksp632I 1075 GAAGAG CTCTTC(1/4)
MaeII 385 ACGT A^CGT
MaeIII 520 GTGAC ^GT[ACGT]AC
MflI 306 GGATCT [GA]^GATC[CT]
MluNI 425 TGGCCA TGG^CCA
MroI 686 TCCGGA T^CCGGA
MscI 425 TGGCCA TGG^CCA
MspI 687 CCGG C^CGG
NsiI 207 ATGCAT ATGCA^T
NspI 741 GCATGT [GA]CATG^[CT]
PvuII 641 CAGCTG CAG^CTG
StyI 862 CCAAGG C^C[AT][AT]GG
Tsp45I 520 GTGAC ^GT[GC]AC
XhoII 306 GGATCT [GA]^GATC[CT]
XmnI 49 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 104 GGTACC G^GTACC
452 GGTACC
AlwI 129 GGATC GGATC(4/5)
306 GGATC
AseI 235 ATTAAT AT^TAAT
396 ATTAAT
AsnI 235 ATTAAT AT^TAAT
396 ATTAAT
Asp718I 104 GGTACC G^GTACC
452 GGTACC
AvaII 554 GGACC G^G[AT]CC
943 GGACC
BanI 104 GGTACC G^G[CT][GA]CC
452 GGTACC
BbvI 729 GCAGC GCAGC(8/12)
189 GCTGC
BciVI 661 GGATAC GTATCC(6/5)
1044 GTATCC
BsmFI 825 GTCCC GGGAC(10/14)
931 GTCCC
BspMI 857 ACCTGC ACCTGC(4/8)
1145 ACCTGC
BstF5I 464 CATCC GGATG(2/0)
163 GGATG
Fnu4HI 189 GCTGC GC^[ACGT]GC
729 GCAGC
FokI 464 CATCC GGATG(9/13)
163 GGATG
HindIII 777 AAGCTT A^AGCTT
840 AAGCTT
ItaI 189 GCTGC GC^[ACGT]GC
729 GCAGC
KpnI 104 GGTACC GGTAC^C
452 GGTACC
MspA1I 100 CAGCGG C[AC]G^C[GT]G
641 CAGCTG
MwoI 732 GCATGATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1132 GCAGTGGTGGC
PshBI 235 ATTAAT AT^TAAT
396 ATTAAT
PstI 1027 CTGCAG CTGCA^G
1130 CTGCAG
TseI 189 GCTGC G^C[AT]GC
729 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
ApoI BfrI BsaBI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstNI
Cfr13I DdeI DpnII EcoRII HaeIII
HinfI HphI MamI MboI MboII
MnlI MseI MvaI NdeII NlaIII
NlaIV PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI TfiI
Tru9I Tsp509I TspRI Van91I