Restriction map for
pFN21AE5057
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI AhdI AluI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbsI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I CelII ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MscI MseI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PpuMI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tru9I Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 77 TCCGGA T^CCGGA
AcsI 499 GAATTC [GA]^AATT[CT]
AcyI 289 GGCGCC G[GA]^CG[CT]C
AfaI 277 GTAC GT^AC
AflIII 94 ACGTGT A^C[GA][CT]GT
AlwI 444 GATCC GGATC(4/5)
AocI 450 CCTCAGG CC^T[ACGT]AGG
ApoI 499 GAATTC [GA]^AATT[CT]
AvaI 250 CCCGGG C^[CT]CG[GA]G
BanI 289 GGCGCC G^G[CT][GA]CC
BbeI 289 GGCGCC GGCGC^C
BbrPI 93 CACGTG CAC^GTG
BbvCI 157 GCTGAGG CCTCAGC(-5/-2)
BcgI 498 CGAATTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 298 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 322 CTGGAG CTGGAG(16/14)
BsaAI 93 CACGTG [CT]AC^GT[GA]
BsaHI 289 GGCGCC G[GA]^CG[CT]C
BsaWI 77 TCCGGA [AT]^CCGG[AT]
BseAI 77 TCCGGA T^CCGGA
BsmFI 339 GTCCC GGGAC(10/14)
BsoBI 250 CCCGGG C^[CT]CG[GA]G
BspEI 77 TCCGGA T^CCGGA
BsrFI 53 GCCGGT [GA]^CCGG[CT]
Bsu36I 450 CCTCAGG CC^T[ACGT]AGG
CfoI 290 GCGC GCG^C
Cfr10I 53 GCCGGT [GA]^CCGG[CT]
Eco81I 450 CCTCAGG CC^T[ACGT]AGG
EcoRI 499 GAATTC G^AATTC
EcoT14I 293 CCTTGG C^C[AT][AT]GG
HaeII 289 GGCGCC [GA]GCGC^[CT]
HhaI 290 GCGC GCG^C
Hin1I 289 GGCGCC G[GA]^CG[CT]C
HinP1I 290 GCGC G^CGC
HinfI 67 GAATC G^A[ACGT]TC
KasI 289 GGCGCC G^GCGCC
MaeII 94 ACGT A^CGT
MaeIII 463 GTCAC ^GT[ACGT]AC
MroI 77 TCCGGA T^CCGGA
MspA1I 537 CAGCGG C[AC]G^C[GT]G
NarI 289 GGCGCC GG^CGCC
PmaCI 93 CACGTG CAC^GTG
PmlI 93 CACGTG CAC^GTG
PshAI 3 GACTGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 277 GTAC GT^AC
SfaNI 557 GCATC GCATC(5/9)
SfiI 297 GGCCGTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 289 GGCGCC GGC^GCC
SmaI 250 CCCGGG CCC^GGG
StuI 28 AGGCCT AGG^CCT
StyI 293 CCTTGG C^C[AT][AT]GG
TfiI 67 GAATC G^A[AT]TC
Tsp45I 463 GTCAC ^GT[GC]AC
Tsp509I 500 AATT ^AATT
XmaCI 250 CCCGGG C^CCGGG
XmaI 250 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BanII 175 GGGCTC G[GA]GC[CT]^C
427 GGGCTC
BbvI 45 GCAGC GCAGC(8/12)
281 GCAGC
BcnI 250 CCCGG CC^[GC]GG
251 CCGGG
BsaI 227 GAGACC GGTCTC(1/5)
126 GGTCTC
BsgI 189 CTGCAC GTGCAG(16/14)
16 GTGCAG
BsmAI 227 GAGAC GTCTC(1/5)
127 GTCTC
BsrI 146 ACTGG ACTGG(1/-1)
342 CCAGT
DdeI 158 CTGAG C^T[ACGT]AG
451 CTCAG
DpnII 216 GATC ^GATC
444 GATC
DsaI 260 CCGTGG C^C[GA][CT]GG
299 CCGTGG
EarI 169 CTCTTC CTCTTC(1/4)
520 CTCTTC
EcoNI 153 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
231 CCTGGGCCAGG
FauI 50 CCCGC CCCGC(4/6)
425 GCGGG
HphI 479 TCACC GGTGA(8/7)
560 TCACC
Ksp632I 169 CTCTTC CTCTTC(1/4)
520 CTCTTC
MboI 216 GATC ^GATC
444 GATC
MboII 170 TCTTC GAAGA(8/7)
521 TCTTC
MslI 93 CACGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
480 CACCCTAATG
NciI 250 CCCGG CC^[GC]GG
251 CCGGG
NdeII 216 GATC ^GATC
444 GATC
Sau3AI 216 GATC ^GATC
444 GATC
TseI 45 GCAGC G^C[AT]GC
281 GCAGC
XcmI 311 CCATTTTTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
389 CCATCCTCCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AvaII BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I EaeI EcoRII
Fnu4HI FokI HaeIII HapII HpaII
ItaI MnlI MspI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI TspRI