##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        AocI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbrPI       BbsI        BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HincII      HindII      HindIII     HinfI       HpaI        
HphI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NcoI        NdeI        
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacII       
SalI        SapI        ScaI        SexAI       SfiI        
SfuI        SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcyI            6  GGCGCC               G[GA]^CG[CT]C
AlwI           24  GATCC                GGATC(4/5)
AlwNI         145  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          382  GGGCCC               GGGCC^C
AvaI          296  CCCGGG               C^[CT]CG[GA]G
BanI            6  GGCGCC               G^G[CT][GA]CC
BbeI            6  GGCGCC               GGCGC^C
BbvCI         364  GCTGAGG              CCTCAGC(-5/-2)
BfaI          163  CTAG                 C^TAG
BsaHI           6  GGCGCC               G[GA]^CG[CT]C
BsoBI         296  CCCGGG               C^[CT]CG[GA]G
BstF5I        354  GGATG                GGATG(2/0)
BstYI          23  AGATCC               [GA]^GATC[CT]
DpnII          24  GATC                 ^GATC
EarI          457  GAAGAG               CTCTTC(1/4)
EcoNI         360  CCTGGCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       182  CCAAGG               C^C[AT][AT]GG
FokI          354  GGATG                GGATG(9/13)
HgaI           91  GACGC                GACGC(5/10)
Hin1I           6  GGCGCC               G[GA]^CG[CT]C
KasI            6  GGCGCC               G^GCGCC
Ksp632I       457  GAAGAG               CTCTTC(1/4)
MaeI          163  CTAG                 C^TAG
MaeIII        398  GTGAC                ^GT[ACGT]AC
MboI           24  GATC                 ^GATC
MboII         457  GAAGA                GAAGA(8/7)
MflI           23  AGATCC               [GA]^GATC[CT]
NarI            6  GGCGCC               GG^CGCC
NdeII          24  GATC                 ^GATC
SacI          274  GAGCTC               GAGCT^C
Sau3AI         24  GATC                 ^GATC
SbfI          246  CCTGCAGG             CCTGCA^GG
SfaNI         355  GATGC                GCATC(5/9)
SfoI            6  GGCGCC               GGC^GCC
SgrAI          58  CACCGGCG             C[GA]^CCGG[CT]G
SmaI          296  CCCGGG               CCC^GGG
Sse8387I      246  CCTGCAGG             CCTGCA^GG
StyI          182  CCAAGG               C^C[AT][AT]GG
Tsp45I        398  GTGAC                ^GT[GC]AC
XhoII          23  AGATCC               [GA]^GATC[CT]
XmaCI         296  CCCGGG               C^CCGGG
XmaI          296  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BanII         274  GAGCTC               G[GA]GC[CT]^C
              382  GGGCCC              
BlpI          301  GCTGAGC              GC^T[ACGT]AGC
              370  GCTGAGC             
Bpu1102I      301  GCTGAGC              GC^T[ACGT]AGC
              370  GCTGAGC             
BssHII         63  GCGCGC               G^CGCGC
              153  GCGCGC              
CelII         301  GCTGAGC              GC^T[ACGT]AGC
              370  GCTGAGC             
DraII         381  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              382  GGGCCCC             
EcoO109I      381  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              382  GGGCCCC             
PvuII         148  CAGCTG               CAG^CTG
              214  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AccII       AciI        AluI        Alw44I      ApaLI       
AspHI       AvaII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmFI       Bsp1286I    BsrFI       BsrI        BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        EaeI        EcoRII      Fnu4HI      
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NgoMIV      NlaIV       PstI        Sau96I      ScrFI       
SfcI        TseI        TspRI