Restriction map for
pFN21AE4127
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AhdI AlwNI AocI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvrII BamHI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BsiWI BsmI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssSI
Bst1107I BstBI BstXI BstZ17I Bsu36I
CelII CpoI DraI DrdI Eam1105I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfcI SfuI SnaBI SpeI SphI
Sse8387I SspBI SwaI TfiI Tsp509I
Tth111I Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1100 GGTACC G^GTACC
AgeI 504 ACCGGT A^CCGGT
Alw44I 967 GTGCAC G^TGCAC
Aor51HI 73 AGCGCT AGC^GCT
ApaLI 967 GTGCAC G^TGCAC
Asp718I 1100 GGTACC G^GTACC
AviII 869 TGCGCA TGC^GCA
BalI 557 TGGCCA TGG^CCA
BbrPI 427 CACGTG CAC^GTG
BbsI 97 GAAGAC GAAGAC(2/6)
BpmI 1345 CTCCAG CTGGAG(16/14)
BpuAI 97 GAAGAC GAAGAC(2/6)
BsaAI 427 CACGTG [CT]AC^GT[GA]
BsaWI 504 ACCGGT [AT]^CCGG[AT]
BsgI 1478 GTGCAG GTGCAG(16/14)
BsmBI 853 GAGACG CGTCTC(1/5)
BspDI 937 ATCGAT AT^CGAT
BstAPI 1406 GCATGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 669 GGTGACC G^GT[ACGT]ACC
BstPI 669 GGTGACC G^GT[ACGT]ACC
BstYI 881 AGATCC [GA]^GATC[CT]
ClaI 937 ATCGAT AT^CGAT
DraIII 396 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 73 AGCGCT AGC^GCT
EcoO65I 669 GGTGACC G^GT[ACGT]ACC
EcoT14I 1247 CCAAGG C^C[AT][AT]GG
FspI 869 TGCGCA TGC^GCA
KpnI 1100 GGTACC GGTAC^C
MflI 881 AGATCC [GA]^GATC[CT]
MluNI 557 TGGCCA TGG^CCA
MscI 557 TGGCCA TGG^CCA
MseI 464 TTAA T^TAA
PaeR7I 886 CTCGAG C^TCGAG
PinAI 504 ACCGGT A^CCGGT
PmaCI 427 CACGTG CAC^GTG
PmlI 427 CACGTG CAC^GTG
PpuMI 1299 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 115 CAGCTG CAG^CTG
SapI 1209 GCTCTTC GCTCTTC(1/4)
SfiI 468 GGCCTTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 503 CACCGGTG C[GA]^CCGG[CT]G
SspI 1551 AATATT AAT^ATT
StuI 467 AGGCCT AGG^CCT
StyI 1247 CCAAGG C^C[AT][AT]GG
Tru9I 464 TTAA T^TAA
XhoI 886 CTCGAG C^TCGAG
XhoII 881 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 606 GATCC GGATC(4/5)
882 GATCC
ApaI 717 GGGCCC GGGCC^C
1261 GGGCCC
BcgI 1425 CGAAGTGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
878 GCAAGATCCTCG
BsiEI 218 CGGCCG CG[GA][CT]^CG
266 CGGCCG
BsmAI 853 GAGAC GTCTC(1/5)
1156 GAGAC
BsrBI 1278 CCGCTC CCGCTC(-3/-3)
1342 CCGCTC
BsrI 874 ACTGG ACTGG(1/-1)
523 CCAGT
BssHII 105 GCGCGC G^CGCGC
838 GCGCGC
DdeI 77 CTAAG C^T[ACGT]AG
582 CTCAG
EagI 218 CGGCCG C^GGCCG
266 CGGCCG
EarI 1210 CTCTTC CTCTTC(1/4)
1239 CTCTTC
EclXI 218 CGGCCG C^GGCCG
266 CGGCCG
Eco52I 218 CGGCCG C^GGCCG
266 CGGCCG
HinfI 1042 GACTC G^A[ACGT]TC
1316 GACTC
Ksp632I 1210 CTCTTC CTCTTC(1/4)
1239 CTCTTC
KspI 254 CCGCGG CCGC^GG
1295 CCGCGG
NaeI 216 GCCGGC GCC^GGC
234 GCCGGC
NgoMIV 216 GCCGGC G^CCGGC
234 GCCGGC
PleI 1042 GACTC GAGTC(4/5)
1316 GACTC
SacII 254 CCGCGG CCGC^GG
1295 CCGCGG
SmaI 1266 CCCGGG CCC^GGG
1332 CCCGGG
SmlI 471 CTTGAG C^T[CT][GA]AG
886 CTCGAG
TspRI 524 CAGTG CA[GC]TG[ACGT][ACGT]^
873 CACTG
XmaCI 1266 CCCGGG C^CCGGG
1332 CCCGGG
XmaI 1266 CCCGGG C^CCGGG
1332 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BfaI BglI
BmyI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI TaqI TseI Tsp45I
TthHB8I