Restriction map for
pFN21AB8968
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII AclI AcsI AflII AflIII
AgeI AhdI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BbrPI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsiHKAI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
MaeI MaeII MaeIII MamI MfeI
MluI MluNI MscI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 350 GGTACC G^GTACC
AccI 1021 GTCTAC GT^[AC][GT]AC
AccIII 459 TCCGGA T^CCGGA
AfaI 351 GTAC GT^AC
AlwI 291 GATCC GGATC(4/5)
AlwNI 994 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 350 GGTACC G^GTACC
BbvCI 142 CCTCAGC CCTCAGC(-5/-2)
BglII 815 AGATCT A^GATCT
BsaWI 459 TCCGGA [AT]^CCGG[AT]
BseAI 459 TCCGGA T^CCGGA
BsgI 790 CTGCAC GTGCAG(16/14)
BsmBI 802 GAGACG CGTCTC(1/5)
BspEI 459 TCCGGA T^CCGGA
BsrBI 71 CCGCTC CCGCTC(-3/-3)
BssSI 931 CACGAG CACGAG(-5/-1)
BstF5I 921 CATCC GGATG(2/0)
BstXI 340 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1033 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
FokI 921 CATCC GGATG(9/13)
KpnI 350 GGTACC GGTAC^C
MroI 459 TCCGGA T^CCGGA
PaeR7I 724 CTCGAG C^TCGAG
PshAI 804 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 962 CTGCAG CTGCA^G
PvuII 853 CAGCTG CAG^CTG
RsaI 351 GTAC GT^AC
SapI 162 GCTCTTC GCTCTTC(1/4)
SfaNI 905 GCATC GCATC(5/9)
SfcI 962 CTGCAG C^T[GA][CT]AG
SfiI 216 GGCCAAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 372 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 10 CCCGGG CCC^GGG
StuI 410 AGGCCT AGG^CCT
TspRI 467 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 218 CCAAGGGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 724 CTCGAG C^TCGAG
XmaCI 10 CCCGGG C^CCGGG
XmaI 10 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcyI 235 GGCGCC G[GA]^CG[CT]C
573 GGCGCC
Aor51HI 332 AGCGCT AGC^GCT
746 AGCGCT
ApaI 224 GGGCCC GGGCC^C
974 GGGCCC
AvaI 10 CCCGGG C^[CT]CG[GA]G
724 CTCGAG
BbeI 235 GGCGCC GGCGC^C
573 GGCGCC
BsaHI 235 GGCGCC G[GA]^CG[CT]C
573 GGCGCC
BsaI 116 GAGACC GGTCTC(1/5)
1041 GGTCTC
BseRI 426 CTCCTC GAGGAG(10/8)
532 GAGGAG
BsmFI 84 GTCCC GGGAC(10/14)
786 GTCCC
BsoBI 10 CCCGGG C^[CT]CG[GA]G
724 CTCGAG
BsrI 632 ACTGG ACTGG(1/-1)
985 CCAGT
BstYI 290 AGATCC [GA]^GATC[CT]
815 AGATCT
EarI 163 CTCTTC CTCTTC(1/4)
1044 CTCTTC
Eco47III 332 AGCGCT AGC^GCT
746 AGCGCT
EcoT14I 77 CCAAGG C^C[AT][AT]GG
218 CCAAGG
Hin1I 235 GGCGCC G[GA]^CG[CT]C
573 GGCGCC
HphI 797 TCACC GGTGA(8/7)
1004 TCACC
KasI 235 GGCGCC G^GCGCC
573 GGCGCC
Ksp632I 163 CTCTTC CTCTTC(1/4)
1044 CTCTTC
MflI 290 AGATCC [GA]^GATC[CT]
815 AGATCT
NarI 235 GGCGCC GG^CGCC
573 GGCGCC
NlaIII 741 CATG CATG^
889 CATG
SfoI 235 GGCGCC GGC^GCC
573 GGCGCC
SmlI 472 CTCAAG C^T[CT][GA]AG
724 CTCGAG
StyI 77 CCAAGG C^C[AT][AT]GG
218 CCAAGG
TaqI 725 TCGA T^CGA
737 TCGA
TthHB8I 725 TCGA T^CGA
737 TCGA
XhoII 290 AGATCC [GA]^GATC[CT]
815 AGATCT
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AluI BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaJI BsiEI BsiYI
BslI BsmAI Bsp1286I BspMI BsrFI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI KspI MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIV PleI SacII Sau3AI
Sau96I ScrFI TseI